6.4 Stage 3 – Test the integrity of the cassette
Before using your cross-flow system and cassette to process product, test the cross-flow system to
ensure it does not leak. Then test the integrity of the cassette.
To test your cross-flow system for leaks, pressurize it with water and check the tubing or piping
connections for leaks. To test the integrity of the cassette, pressurize the system with compressed
air and measure the diffusional flow of air through the cassette membrane. A high flow rate (above
the cassette’s specification) indicates there might be a hole or tear in the membrane.
Follow the instructions below to test the system for leaks, and to test the integrity of the cassette.
Test your system for leaks
To test your cross-flow system for leaks, fill the system with clean water. Open the feed valve. Open
the retentate valve. Close the permeate valve. Start the feed pump on slow speed and throttle the
retentate valve until the feed pressure reads 3 barg (45 psig). Check all connections for leaks.
Test the integrity of the cassette
To measure diffusional air flow through the membrane via the permeate port, you will need to add a
compressed air line, pressure regulator, and shutoff valve to your cross-flow system. You also need
the following equipment: a 500-ml beaker, 50-ml graduated cylinder, stopwatch, and a piece of
flexible tubing (
).
Test the integrity of the system and cassette manually by following the procedure described below.
6 Preparing a Cassette for Use
6.4 Stage 3 – Test the integrity of the cassette
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Learning to Use the Kvick Lab Cassette Holder User Manual 18117269 AI
