Step
Action
4
Open the drain valve, and with the retentate valve open, start-up the pump at a slow
speed. Apply backpressure by slightly closing the retentate valve if needed to assist in
removal of product from the drain line. Stop the pump as the tank fully drains.
5
Close the drain valve. Add a portion of saved permeate to the feed tank and circulate for
two to three minutes. Open the drain valve and circulate (and apply backpressure by
slightly closing the retentate valve if needed) to drain the product from the feed-
retentate loop. Stop the pump. Repeat step 5 as needed to recover the maximum volume
of product.
Retentate
Pump
Valve
Pressure
Feed
gauge
Feed reservoir
Kvick Lab
holder
Collection
vessel
Permeate
Drain
line
Collection
vessel
As you near the end of processing,
collect 200 to 500 ml of permeate
for flushing the system.
After recovering product
from the drain line, add a
portion of the collected
permeate, circulate, and
drain into your product
collection vessel.
Figure 7.2: Typical process flow path for shutting the system down and recovering product
7 Using the Holder
7.4 Stage 3 – Shutting the system down and recovering product
46
Learning to Use the Kvick Lab Cassette Holder User Manual 18117269 AI
