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setting when counting larger cells if they are being counted as multiple smaller cells.
Decrease if small cells are not being counted.
o
Maximum Diameter -
Objects above the maximum diameter will not be counted. Adjust the
maximum diameter to provide a realistic size band to analyze during counts. If clusters of
cells are being counted as a large individual cell, reducing the maximum diameter to reflect
the true cell size will improve the results.
•
Roundness:
CellDrop uses Roundness settings to identify cells based on morphology. The
higher the roundness, the more round and completely outlined a cell must be to be included in
the count. Reducing the roundness setting allows more elliptically shaped cells, irregularly
shaped cells or cells with incomplete structures to be counted. If single cells are counted as
multiple cells or artifacts are counted, increase the roundness setting. If cells are not counted,
decrease the roundness.
o
Brightfield Roundness - Used for Brightfield counts.
o
Live Roundness - Used in apps counting live cells.
o
Dead Roundness - Used in apps counting dead cells.
o
Green Roundness - Used in the Custom Methods app for the green fluorescence channel.
o
Red Roundness - Used in the Custom Methods app for the red fluorescence channel.
o
FL Roundness - Used in the GFP app to count fluorescing cells.
•
Threshold:
The CellDrop Series uses Threshold settings to evaluate each counted cell to see if
it is above the intensity criteria set in the protocol. The higher the threshold is, the greater the
intensity must be to be included in the count.
o
Stained Threshold - Used in the Trypan Blue app to determine if a cell is dead or alive. The
higher the setting, the darker a cell must be to be counted as dead. If dead cells are not
being counted, decrease the threshold. If live cells are being counted as dead, increase the
threshold value.
o
Green Fluorescence Threshold - Used in apps that count cells using the green
fluorescence channel.
o
Red Fluorescence Threshold - Used in apps that count cells using the red fluorescence
channel.
o
Decrease the fluorescence threshold if cells that are positively stained are not being
counted in the analysis and vice versa.
Summary of Contents for CellDrop FL
Page 1: ...December 2019...
