DeNovix CellDrop FL, User Manual

Page 1: ...December 2019...

Page 2: ...11 Custom Methods 12 6 Count Tab View 13 Sample Name and Sample Information 13 Image Controls 13 Dilution Calculator 14 Count Button 15 7 Count Settings 16 8 Results Tab View 18 Optimize Settings 18 N...

Page 3: ...ide is designed for both CellDrop BF Brightfield only and CellDrop FL Dual Fluorescence plus Brightfield owners Several applications and software features are only applicable for instruments with Fluo...

Page 4: ...Propidium Iodide and other reagents are known hazardous materials Consult Safety Datasheets SDS for these assays prior to use Biological samples such as tissues and bodily fluids of humans and other a...

Page 5: ...t rieur dans les conditions suivantes Temp rature 15 35 C Humidit 35 65 Utilisez uniquement le bloc d alimentation inclus avec l instrument Utiliser l appareil dans des conditions non sp cifi es par l...

Page 6: ...ouch the focus icon and then adjust while viewing the Brightfield channel Live cells should have a bright center and a dark outline The image below on the left is correctly focused The image on the ri...

Page 7: ...t Practices for additional details Ensure that surfaces are clean prior to loading a sample into the sample chamber If a previous sample has dried on the surfaces or if the sample is not loading easil...

Page 8: ...is the default account for CellDrop applications Tap the account name to display a list of available accounts Changing an account will automatically use the protocol indicated as the default option se...

Page 9: ...s a guideline of the best chamber height to choose based on the density of the sample Gap Height Required Volume Minimum Density cells mL Maximum Density cells mL 400 um 40 uL 7 x 102 1 x 105 100 um 1...

Page 10: ...a defined border The application is not designed to count dark solid objects Trypan Blue Trypan blue is a cell membrane impermeable dye that only enters cells with compromised membranes Upon entry the...

Page 11: ...luorescence resonance energy transfer FRET Viability AO count AO count PI count 100 GFP The GFP App uses green fluorescent images to count GFP transfected cells and brightfield images to count non tra...

Page 12: ...on Viability FDA count FDA count PI count 100 Custom Methods The Custom Methods app allows users to count cells using brightfield green fluorescence or red fluorescence techniques The application is i...

Page 13: ...s within this application Users may set a custom protocol as their default in the User Accounts app Image Controls Channel Selector For the CellDrop FL model use the Channel Selector to switch between...

Page 14: ...posed Adjust the exposure for each channel independently Zoom Controls All preview and result images on the CellDrop support zoom and pan capabilities Pinch and zoom using two fingers to zoom in or ou...

Page 15: ...n guidance presented under the Single Sample view as well as the Results tab Results List view when the Show Dilution Guidance box is checked Count Button The Count button initiates a measurement The...

Page 16: ...00 um 10 uL 5 x 104 1 x 107 50 um 5 uL 1 x 107 2 5 x 107 Small Cell Mode Small Cell Mode can improve brightfield channel counts of small cells near the 4 um limit of detection The additional algorithm...

Page 17: ...unts o Live Roundness Used in apps counting live cells o Dead Roundness Used in apps counting dead cells o Green Roundness Used in the Custom Methods app for the green fluorescence channel o Red Round...

Page 18: ...using the new settings Users have the option to save the adjusted settings to a protocol if desired Next Count This button changes the view back to the Count tab and starts a live preview The previou...

Page 19: ...sult Overlay Selector Brightfield Brightfield Green Overlay Green Fluorescence Brightfield Red Overlay Red Fluorescence Green Red Overlay Brightfield Green Red Overlay Action Bar Controls View Selecto...

Page 20: ...esults Panel A table of results will be displayed in the Results panel If dilution parameters were provided at the time the count was initiated then dilution guidance will also be presented in the pan...

Page 21: ...h showing totals only or with a cell size distribution histogram Total Cell Count Available for the Brightfield and GFP apps only Live Cell Count Available for all apps except Brightfield Dead Cell Co...

Page 22: ...al sample related information not displayed in the Results tab Single Sample view is included when exporting in a csv format o pdf The results and protocol settings will be presented in a table format...

Page 23: ...dding or Deleting Accounts There are two preconfigured system accounts built into the software the General Account and the Primary Administrator Account These accounts may not be deleted Primary Admin...

Page 24: ...ccount The account specific saved data will also be permanently removed from the instrument at the time the account is deleted Data This app allows the user to search for all saved data filtering my d...

Page 25: ...t Codes The purpose of the Count Codes app is to manage pre paid counts using codes on a per account basis This app will appear on instruments purchased on a pay as you go basis The Primary Administra...

Page 26: ...Automatic Archive Use this selection to enable or disable all standard accounts including the General Account to archive data from the instrument Refer to the Delete Data section for additional infor...

Page 27: ...y be downloaded using the Updater app when connected to Ethernet or Wifi An Ethernet connection is recommended for software updates Updates maybe downloaded on line or downloaded to a FAT32 formatted...

Page 28: ...ccurring software error Contact DeNovix Customer Support at 302 442 6911 or info denovix com if additional help is required Please include the serial number of your instrument and all pertinent data w...

Page 29: ...return email address Please note responses will not be sent directly to the email account used by the instrument Outside of the US please contact your local distributor for assistance Warranty All pr...