DeNovix CellDrop, User Manual

Page 1: ...rev MAR 27 2019 ...

Page 2: ...Screen 11 Sample Information A 11 Image Controls 12 Dilution Calculator Settings E 12 Count Button 12 6 Results Tab Views 13 Blue Action Bar 13 Single Sample Results View 14 Results List View 14 Image List View 14 Graph View 14 7 Export and Print Options 16 Screen Capture 16 Export 16 8 Utility Apps 17 Accounts App 17 Data App 18 Count Codes App 19 Calculator App 19 Timer App 20 Settings App 20 Up...

Page 3: ... for both CellDrop BF Brightfield only and CellDrop FL Dual Fluorescence plus Brightfield owners Several applications and software features are only applicable for instruments with Fluorescence mode CellDrop FL and do not apply to the CellDrop BF Key Features No consumable slides required Integrated processor and Hi Definition 7 inch touchscreen Custom software interface with intuitive EasyApps Va...

Page 4: ...e Orange Propidium Iodide and other reagents are known hazardous materials Consult Safety Datasheets SDS for these assays prior to use Biological samples such as tissues and bodily fluids of humans and other animals have the potential to transmit infectious diseases Follow all applicable local regulations Wear appropriate personal protective equipment PPE Read and follow the guidelines in this Use...

Page 5: ...térieur dans les conditions suivantes Température 15 à 35 C Humidité 35 à 65 Utilisez uniquement le bloc d alimentation inclus avec l instrument Utiliser l appareil dans des conditions non spécifiées par le fabricant peut compromettre la protection offerte par le cable d alimentation et le bloc d alimentation fournis avec l appareil VCCI Class B compliance statement for users in Japan This is a Cl...

Page 6: ...ions fall within concentration limits of the instrument for accurate results o Use the appropriate protocol settings for Chamber Height when measuring high or low concentration samples or when using slides o Note Refer to the text located in the Count button background field for the proper loading volume when measuring low or high density samples Lower the arm before dispensing the sample onto the...

Page 7: ...ncluding Timer finished alert and low count alerts as set in the Count Codes app Pay As You Go Models Account The current account in use is displayed on the top action bar Tap the account name to display a list of available accounts Changing an account will automatically use the protocol indicated as the default option selected in the Accounts app accessed from the Home screen Home Icon Tapping th...

Page 8: ..._____________________________ Dilution factor of squares counted 10 000 cells mL Minimum and Maximum Recommended Concentrations Gap Height Required Volume Minimum Cells mL Maximum Expected Cells mL 400 um 40 uL 7 x 102 5 x 104 100 um 10 uL 5 x 104 1 x 107 50 um 5 uL 1 x 107 2 5 x 107 Data Storage CellDrop models include a solid state drive with 60 GB or more of storage capacity sufficient for seve...

Page 9: ...rypan Low Exposure setting Trypan Blue Threshold The brightfield channel captures a monochrome image so the darker cells stained with trypan have a lower intensity Therefore the trypan blue threshold setting is the intensity value from 1 100 at which anything below is counted as a dead cell AO App The AO App uses green fluorescence images to count live nucleated cells Acridine orange is a nuclear ...

Page 10: ... green fluorescent protein GFP isolated from the Aequorea victoria jellyfish is a useful reporter molecule for monitoring gene expression GFP emits green light lambda max 509 nm when excited with UV or blue light resulting in cells that green fluorescent cells The GFP app is used to determine the transfection efficiency of transient transfections Efficiency GFP count GFP count Brightfield count 10...

Page 11: ...ture of 5 uL of assay working solution to 5 uL of cell suspension set Dilution Factor 2 o For a mixture of 8 uL of assay working solution to 2 mL of cell suspension set Dilution Factor 5 Minimum and Maximum Diameters The size range for cells that will be included in a count are set in the protocol Cells outside this range will not be shown on histograms The allowable range is 2 400 μm Roundness Th...

Page 12: ...upport zoom and pan capabilities Use two fingers to zoom in or out Tap and drag the image to move it around Image map F A small position map in the top right is an indicator of what portion of the full image is currently displayed on the screen Expand Collapse Icon G Tapping the expand icon located on the top right of all preview and result screens will result in the image filling the entire scree...

Page 13: ...P results may be gated by intensity as well as size Histogram icon This icon opens a histogram of Cell Number versus Cell Diameter for a given count Image Selector This icon indicates which channel s image brightfield green red or composite is currently displayed Tap the icon to display the channel selector feature The selected image determines which composite result image is exported Calculator i...

Page 14: ...ture a new image to count The previous results will be overwritten To capture a new result of a new sample raise the arm clean the upper and lower sample surfaces and lower the arm to load the next sample Results List View List The list includes all samples counted in the current measurement session When the Show Dilution Guidance box is checked the dilution guidance is displayed with each result ...

Page 15: ...GFP Cell Count Available for the GFP app only Without cell size distribution Cluster Available for all apps Displays count of cell size clusters Viability Available for Trypan Blue PI AO PI and Yeast apps ...

Page 16: ...e related information not displayed in the Results tab Single Sample view is included when exporting in a csv format o pdf The results and protocol settings will be presented in a table format The results table will include only the fields included on the Results tab Single Sample View o png The selected images will be exported as png files When results are not included in the export selection ima...

Page 17: ... system accounts built into the software the General Account and the Primary Administrator Account These accounts may not be deleted Primary Administrator This account is used to manage instrument settings It is not used within the count applications The primary administrator account is not initially password protected A password may be applied at any time General Account This account is used as t...

Page 18: ...and custom report formats created by that account are reassigned to the General Account The account specific saved data will also be permanently removed from the instrument at the time the account is deleted Data App This app allows the user to search for past data filtering my date app name protocol name and or sample names Data can then be added to the Result tab screens for review and export In...

Page 19: ...user interface and update the progress message If the Cancel button is tapped during the archiving process samples already copied up to the stop point may be deleted from the instrument at the user s discretion Count Codes App The purpose of the Count Codes app is to manage pre paid counts using codes on a per account basis This app will appear on instruments purchased on a pay as you go basis The...

Page 20: ...ecific text will be available in a limited number of language options Network Information The WiFi and Ethernet MAC addresses and IP addresses can be found under this setting Permissions This selection is used to enable the following options Automatic Archive Use this selection to enable or disable all standard accounts including the General Account to archive data from the instrument Refer to the...

Page 21: ... section on the left scroll menu 4 Enable the POP3 option on your Email configuration Do not use an IMAP account as messages from IMAP accounts could occupy a significant amount of the instrument s memory 5 Input Outgoing Server Information Email Configuration Example Input for Gmail SMTP Server smtp gmail com Security SSL Port 465 Email Username example gmail com Email Password Examplegmail123 Te...

Page 22: ...ally Avoid introducing air bubbles into the sample solution when mixing or dispensing samples Refer to the manufacturer of cell dyes such as Acridine Orange Propidium Iodine or FDA for assay specific troubleshooting guidance Software Errors Pop up messages will provide guidance for software based errors or invalid user actions In most cases simply re launching the app will allow the user to contin...

Page 23: ...sable swiping motions from activating other software activities Tap the home button to exit Solvent Compatibility The CellDrop are compatible with most solvents typically used in life science laboratories Exception Do not use Hydrofluoric Acid HF as the fluoride ion will etch the surfaces of the measurement surfaces ...

Page 24: ...d return email address Please note responses will not be sent directly to the email account used by the instrument Outside of the US please contact your local distributor for assistance Warranty All product s and accessories sold by DeNovix Inc are under warranty against manufacturing defects Disclaimer All information in this document is for reference purposes only DeNovix Inc makes no claims tha...