B.2.6 Column Installation
The separator columns are purchased separately from the
LC20.
1. Before installing the separator columns, pump deionized
water through the LC20 at 3 mL/min for 1 to 2 minutes to
clear any air from the liquid lines. Activate injection each
valve several times to make sure that no air is trapped in
the hydraulic system. Trapped air will reduce efficiency.
2. Reduce the flow rate to 2.0 mL/min and verify that the
pressure through the system, with no columns installed, is
less than 690 KPa (100 psi).
3. Install the separator column mounting clip by inserting it
into one of the square holes at the front edge of the
component panel (see Figure B-7. If you are using the
4-mm suppressor columns, orient the clip so the larger
clips are toward the front. If you are using the 2-mm
columns, orient the clip so the smaller clips are nearer the
front.
Figure B-7. Separator Column Mounting Clip
LC20 Chromatography Enclosure
B-12
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Summary of Contents for LC20
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