Dionex UltiMate 3000 LCi, Quick Installation Manual

Page 1: ...January 2010 Rev 02 2010 Dionex UltiMate 3000 Viper Capillary Kits for Intelligent LC LCi System Solutions Quick Installation Guides ...

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Page 3: ...al Ternary Standard P N 6040 2806 or Rapid Separation P N 6040 2805 17 3 1 Benefits of Application Switching 17 3 2 Scope of Delivery 17 3 3 Stacking the Modules 18 3 4 Flow Schematic 19 4 Viper On Line SPE Capillary Kits Dual Ternary Standard P N 6040 2802 or Rapid Separation P N 6040 2801 21 4 1 Benefits of On line SPE 21 4 2 Scope of Delivery 21 4 3 Stacking the Modules 22 4 4 Flow Schematic 23...

Page 4: ...gent LC LCi System Solutions Quick Installation Guides Page ii Rev 02 January 2010 2010 Dionex 6 3 2 Dual stack with Extension Kit 32 6 4 Flow Schematics 33 6 4 1 Single stack without Extension Kit 33 6 4 2 Dual stack with Extension Kit 35 Parts 37 ...

Page 5: ...0 mm All delivered kits work for common chromatographic scenarios For extreme individual requirements e g combining a 30 mm column with a 300 mm column in Parallel LC it might be necessary to complement certain connections with additional capillaries Each chapter provides a recommended schematic system setup In general the system setup in a dual stack configuration is also possible however this mi...

Page 6: ...mistries to solve typical analytical challenges such as method development sample preparation or increasing throughput and productivity 1 3 Typical System Setups 1 3 1 Single Stack In many cases bench space is limited hence the Dionex UltiMate 3000 systems are designed to consume as little space as possible We assume that a single stack setup is the most common case therefore the LCi capillary kit...

Page 7: ...umns and moderate pressures mediocre connections can cause retention time shifts and peak distortions Conventional stainless steel HPLC connectors use a ferrule and a nut to establish connections A flaw of the conventional design is that a void volume free connection is not guaranteed particularly when changing the tubing between differently shaped threads e g when changing a column Viper connecto...

Page 8: ...ections seal properly Usually the Viper fitting is tight for pressures up to 1 200 bar 17 400 psi after the first attempt If leakage occurs under these conditions tighten the screw s gradually further until the connection seals properly Do not turn the screw beyond an angle of 90 to avoid damages of the PEEK seal by over tightening Do not apply brute force To extend the life time of Viper open and...

Page 9: ...ms are delivered with a dedicated set of 3 Viper capillaries for system plumbing In addition dedicated Viper capillary RS and SD kits containing 3 capillaries each are available to equip Dionex UltiMate 3000 systems with Viper Figure 6 Packaging box for Viper RS and SD capillary kits for UltiMate 3000 systems single system see Figure 1 The opposite side of the foam inlay allows for shipment of Vip...

Page 10: ...7 x 450 mm 1 pc Viper capillary SST ID x L 0 13 mm 0 005 x 350 mm 1 pc Viper capillary SST ID x L 0 13 mm 0 005 x 250 mm 1 pc Viper Installation and Operation Guide short version 1 pc 1 5 1 1 2 Viper Capillary Kit SD System Table 2 Scope of delivery for the Viper capillary kit SD system P N 6040 2302 Description Quantity Viper capillary SST ID x L 0 18 mm 0 007 x 450 mm 1 pc Viper capillary SST ID...

Page 11: ... 2010 Dionex 1 5 2 LCi Capillary Kits All Viper LCi capillary kits are delivered in a high quality box with a foam inlay Independent of the ordered kit you will always receive the same box accommodating all necessary accessories and capillaries for your individual LCi kit Figure 7 Packaging box for all Viper LCi capillary kits ...

Page 12: ...010 Dionex 1 6 Viper Labeling Each Viper capillary comes with a labeling clip This clip can be easily detached and reattached to 1 16 and 1 32 OD capillaries In addition a set of 5 blank clips and stickers is supplied for individual labeling purposes Figure 8 Removable labeling clip with sticker for individual capillary labeling ...

Page 13: ...me changes in temperature such as direct sunlight or drafts and high air humidity Note For information about the power and communication connections LAN USB as well as the installation procedures for other components such as the Active Rear Seal Wash System please refer to the operating instructions of the system components 1 8 Connecting the Eluent Line to the Solvent Reservoir 1 Feed the eluent ...

Page 14: ...nt Always use eluent tubing with filter frits for drawing eluents from the eluent bottles This prevents contamination of the HPLC system Note Regularly check the frits for permeability Especially when working with aqueous solvents algae and other microorganisms can grow on the frits causing clogging Therefore exchange the solvents at regular intervals Rinse the bottles thoroughly with fresh eluent...

Page 15: ...pillary connection to shortcut ports 7 and 10 The 6 port valve has a direct fluidic connection between port 1 and port 6 To achieve this fluidic connection for a 10 port valve Dionex recommends connecting ports 7 and 10 with an additional suitable capillary The inner diameter of this capillary should be 0 13 mm 0 005 for a rapid separation RS setup or 0 18 mm 0 007 for a standard SD setup Ports 8 ...

Page 16: ...UltiMate 3000 Viper Capillary Kits for Intelligent LC LCi System Solutions Quick Installation Guides Page 12 Rev 02 January 2010 2010 Dionex ...

Page 17: ...le Dionex detector all of which are fully controlled by the Chromeleon Chromatography Data System Prior to installation please consider chapter 1 for important additional information 2 2 Scope of Delivery Table 3 Scope of delivery for the Viper capillary kit RS system Tandem Operation SST P N 6040 2803 and Viper capillary kit SD system Tandem Operation SST P N 6040 2804 Description Quantity Viper ...

Page 18: ...ll capillaries inside the modules as needed SRD 3600 Solvent Rack and Degasser DGP 3600RS SD Dual Gradient Pump WPS 3000 T SL T RS inline Split Loop Autosampler TCC 3000RS SD Thermostatted Column Compartment with 1x 2 position 10 port switching valve right Stackable Dionex Detector Important All modules are primed with 2 propanol at the factory Prior to initial operation of the system make sure th...

Page 19: ...S sampler valve port 5 0 18 mm 0 007 x 450 mm TCC 10 port valve right port 7 Column 1 inlet RS 0 13 mm 0 005 x 250 mm SD 0 18 mm 0 007 x 250 mm TCC 10 port valve right port 5 Column 2 inlet RS 0 13 mm 0 005 x 250 mm SD 0 18 mm 0 007 x 250 mm TCC 10 port valve right port 4 TCC 10 port valve right port 9 RS 0 13 mm 0 005 x 150 mm SD 0 18 mm 0 007 x 150 mm TCC 10 port valve right port 3 Waste 0 25 mm...

Page 20: ...0 Dionex Note In case of a dual stack setup the TCC 10 port valve right turns into a TCC 10 port valve left Both stacks should have a distance of about 7 cm 2 8 inches Connecting a post column cooler with a stackable Dionex detector does not work without additional capillaries when setting up the system in the U configuration 1 3 2 ...

Page 21: ...2 position 6 port switching valve left for column switching a split loop well plate autosampler and a stackable Dionex detector all of which are fully controlled by the Chromeleon Chromatography Data System Prior to installation please consider chapter 1 for important additional information 3 2 Scope of Delivery Table 5 Scope of delivery for the Viper capillary kit RS system Application Switching ...

Page 22: ...e modules as needed Important All modules are primed with 2 propanol at the factory Prior to initial operation of the system make sure that all used solvents are miscible with 2 propanol and equilibrate your system Otherwise perform appropriate intermediate flushing steps SRD 3600 Solvent Rack and Degasser DGP 3600RS SD Dual Gradient Pump WPS 3000 T SL T RS inline Split Loop Autosampler TCC 3000RS...

Page 23: ...010 Rev 02 Page 19 2010 Dionex 3 4 Flow Schematic The figure below shows the schematic setup of the application switching configuration Install your Viper capillaries according to the information of Table 6 and the system stack schematic 3 3 Figure 13 Flow schematic of the automated application switching setup ...

Page 24: ...port valve left port 2 RS 0 13 mm 0 005 x 650 mm SD 0 18 mm 0 007 x 650 mm WPS sampler valve port 4 TCC 6 port valve left port 1 RS 0 13 mm 0 005 x 750 mm SD 0 18 mm 0 007 x 750 mm TCC 10 port valve right port 7 Waste 0 25 mm 0 010 x 1 000 mm PEEK TCC 10 port valve right port 9 Waste 0 25 mm 0 010 x 1 000 mm PEEK Column 1 outlet TCC 6 port valve left port 5 RS 0 13 mm 0 005 x 250 mm SD 0 18 mm 0 0...

Page 25: ... switching a split loop well plate autosampler and a stackable Dionex detector all of which are fully controlled by the Chromeleon Chromatography Data System Prior to installation please consider chapter 1 for important additional information 4 2 Scope of Delivery Table 7 Scope of delivery for the Viper capillary kit RS system on line SPE SST P N 6040 2801 and Viper capillary kit SD system on line...

Page 26: ...g all capillaries inside the modules as needed SRD 3600 Solvent Rack and Degasser DGP 3600RS SD Dual Gradient Pump WPS 3000 T SL T RS inline Split Loop Autosampler TCC 3000RS SD Thermostatted Column Compartment with 1x 2 position 6 port switching valve Stackable Dionex Detector Important All modules are primed with 2 propanol at the factory Prior to initial operation of the system make sure that a...

Page 27: ...p Description of Connections Capillary Dimensions ID x L PumpLeft outlet TCC 6 port valve right port 5 0 18 mm 0 007 x 950 mm PumpRight outlet WPS sampler valve port 5 0 18 mm 0 007 x 450 mm TCC 6 port valve right port 3 SPE column inlet RS 0 13 mm 0 005 x 250 mm SD 0 18 mm 0 007 x 250 mm TCC 6 port valve right port 4 Column inlet RS 0 13 mm 0 005 x 250 mm SD 0 18 mm 0 007 x 250 mm WPS sampler val...

Page 28: ...10 Dionex Note In case of a dual stack setup the TCC 6 port valve right turns into a TCC 6 port valve left Both stacks should have a distance of about 7 cm 2 8 inches Connecting a post column cooler with a stackable Dionex detector does not work without additional capillaries when setting up the system in the U configuration 1 3 2 ...

Page 29: ...t the same time a thermostatted column compartment with one 2 position 6 port switching valve for column switching a split loop well plate autosampler and two stackable Dionex detectors all of which are fully controlled by the Chromeleon Chromatography Data System Prior to installation please consider chapter 1 for important additional information 5 2 Scope of Delivery Table 9 Scope of delivery fo...

Page 30: ...00 Solvent Rack and Degasser DGP 3600RS SD Dual Gradient Pump WPS 3000 T SL T RS inline Split Loop Autosampler Combination of two stackable Dionex Detectors TCC 3000RS SD Thermostatted Column Compartment with 1x 2 position 6 port switching valve The recommended distance between the two stacks is approximately 6 8 cm 2 4 3 2 inches Important All modules are primed with 2 propanol at the factory Pri...

Page 31: ...Note The eluents in both systems have to be fully miscible both columns have to be operated at the same column temperature as they are located in the same column compartment the initial eluent compositions on both columns should be comparable to support fast flow path switching comparable cycle times for both methods are advantageous To ease the installation of the capillaries it is helpful to est...

Page 32: ...outlet Detector 2 inlet RS 0 13 mm 0 005 x 350 mm SD 0 18 mm 0 007 x 350 mm Note In case of a single stack setup of the Parallel LC system the TCC 6 port valve left turns into a TCC 6 port valve right The capillary lengths have to be adapted as outlined in Table 11 Table 11 Detailed description of capillary connections of the Parallel LC setup single stack Description of Connections Capillary Dime...

Page 33: ...thout the extension kit is the single stack whereas the extension kit requires the dual stack setup 5 3 The HPLC system with Automated Method Scouting includes a quaternary gradient pump a thermostatted column compartment with two 6 position 7 port switching valves for column switching a split loop well plate autosampler and preferably a diode array detector all of which are fully controlled by th...

Page 34: ... 3000 Solvent Rack without degasser 1 pc Solvent selection valve 10 pos 11 port 1 pc Mounting plate for solvent selection valve 1 pc Viper capillary SST ID x L 0 13 mm 0 007 x 650 mm 1 pc Viper capillary SST ID x L 0 18 mm 0 007 x 650 mm 1 pc Viper capillary SST ID x L 0 13 mm 0 007 x 150 mm 1 pc Viper capillary SST ID x L 0 18 mm 0 007 x 150 mm 1 pc Viper union for direct connection of two Viper ...

Page 35: ...x recommends installing all capillaries inside the modules as needed SRD 3600 Solvent Rack and Degasser LPG 3400RS SD Quaternary Pump WPS 3000 T SL T RS inline Split Loop Autosampler TCC 3000RS SD Thermostatted Column Compartment with 2x 6 position 7 port switching valves Stackable Dionex Detector Important All modules are primed with 2 propanol at the factory Prior to initial operation of the sys...

Page 36: ...to 9 additional solvent bottles and the solvent selection valve to be placed on top of the system Solvent Selection Valve with mounting plate SR 3000 Solvent Rack DAD 3000 RS Diode Array Detector recommended TCC 3000RS SD Thermostatted Column Compart ment with 2x 6 pos 7 port switching valves SRD 3600 Solvent Rack and Degasser LPG 3400RS SD Quaternary Pump WPS 3000 T SL T RS inline Split Loop Auto...

Page 37: ...4 and the system stack schematic 6 3 1 Note All columns have to be operated at the same column temperature in order to allow for fast and easy system flushing one column can be replaced with a suitable capillary as a bypass not included in delivery or a column can be replaced by a Viper union to directly connect the two valves The kit contains colored capillary clips for color coding of the capill...

Page 38: ...0 mm TCC 7 port valve right port 6 Column 6 inlet RS 0 13 mm 0 005 x 350 mm SD 0 18 mm 0 007 x 350 mm Column 1 outlet TCC 7 port valve left port 1 RS 0 13 mm 0 005 x 250 mm SD 0 18 mm 0 007 x 250 mm Column 2 outlet TCC 7 port valve left port 2 RS 0 13 mm 0 005 x 250 mm SD 0 18 mm 0 007 x 250 mm Column 3 outlet TCC 7 port valve left port 3 RS 0 13 mm 0 005 x 250 mm SD 0 18 mm 0 007 x 250 mm Column ...

Page 39: ...nd the system stack schematic 6 3 2 Note All columns have to be operated at the same column temperature in order to allow for fast and easy system flushing one column can be replaced with a suitable capillary as a bypass not included in delivery or a column can be replaced by a Viper union to directly connect the two valves The delivery contains colored capillary clips for color coding of the capi...

Page 40: ...ort 6 Column 6 inlet RS 0 13 mm 0 005 x 350 mm SD 0 18 mm 0 007 x 350 mm Column 1 outlet TCC 7 port valve left port 1 RS 0 13 mm 0 005 x 250 mm SD 0 18 mm 0 007 x 250 mm Column 2 outlet TCC 7 port valve left port 2 RS 0 13 mm 0 005 x 250 mm SD 0 18 mm 0 007 x 250 mm Column 3 outlet TCC 7 port valve left port 3 RS 0 13 mm 0 005 x 250 mm SD 0 18 mm 0 007 x 250 mm Column 4 outlet TCC 7 port valve lef...

Page 41: ...040 2385 Viper SST Flex Cap ID L 0 18 mm 0 007 x 350mm SST 6040 2375 Viper SST Flex Cap ID L 0 18 mm 0 007 x 450mm SST 6040 2365 Viper SST Flex Cap ID L 0 18 mm 0 007 x 550mm SST 6040 2355 Viper SST Flex Cap ID L 0 18 mm 0 007 x 650mm SST 6040 2395 Viper SST Flex Cap ID L 0 18 mm 0 007 x 750mm SST 6040 2370 Viper SST Flex Cap ID L 0 18 mm 0 007 x 850mm SST 6040 2380 Viper SST Flex Cap ID L 0 18 mm...

Page 42: ...2 Retaining guide for solvent tubing 6000 0042 Sealing plug for eluent bottle screw cap 6000 0047 Capillary labeling clips for 1 16 and 1 32 OD capillary 10 pcs 6040 2399 Filter frit SST porosity 10 μm 6268 0110 Viper union for direct connection of two Viper capillaries 6040 2304 Viper plug SST 6040 2303 Viper Capillary Kit RS system 6040 2301 Viper Capillary Kit SD system 6040 2302 ...