up on the curved part of the pipe, turning it 90° counter-clockwise and gently lowering it into the chamber, see
Figure 2.2. A chamber can then be emptied by pressing the corresponding numbered button. Pr
essing the “all”
button will drain all the chambers at the same time.
4.
Chamber Covers
: The chamber covers will help maintain the temperature and other buffer conditions (gas
tension, pH) fairly constant. Holes in the chamber covers serve different purposes, and they are illustrated in
Figure 2.3A-B. The slots allow the covers to be placed over the chamber around the support arms and gas/
vacuum tubes.
Figure 2.3A - Chamber cover
Figure 2.4B - Chamber cover with built-in electrodes (optional item)
5.
Initial Force Transducer Status:
Prior to the shipment of the Organ Bath 820MO System, it has gone
through two days of continuous testing, including a final force transducer calibration. However, DMT
recommends that a new force transducer calibration is performed before using the organ bath system for the
first time. The force transducer calibration procedure is described in detail in the FORCE CALIBRATION sub-
menu under SETTINGS, as explained in Chapter 3.
12
NOTE: WHEN DRAINING THE CHAMBERS USING THE AUTOMATIC VACUUM FUNCTION,
PRESS THE APPROPRIATE BUTTON FOR AN ADDITIONAL 3-5 SECONDS AFTER THE INITIAL
EMPTYING. THIS WILL HELP DRAIN RESIDUAL BUFFER AND SOLUTIONS RETAINED IN THE
TUBING AND VALVES.
Figure 2.2 Oxygen supply and suction connection
Gas
Suction
Summary of Contents for 820MO
Page 1: ...ORGAN BATH SYSTEM 820MO USER MANUAL VOL 1 2 ...
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