MICROMANIPULATORS
Check the micropositioner for grease at least once a month. There are 3 main parts that will need to be greased
on the micropositioner, those parts being the micropositioner screw thread and the 2 slide bars to the left and right
of the micropositioner screw thread. In case of insufficient lubrication, grease the micropositioner with the
“Grease
for Linear
Slides” included with your system. Apply the linear slide grease in the areas indicated by the arrows in
figure
4.6 below.
Figure 4.6 The areas where linear slide grease may be applied for smooth micropositioner movement
4.5
CLEANING THE ORGAN BATH
DMT strongly recommends that the organ bath chambers and surrounding areas are cleaned after each experiment.
At the end of each experiment, use the following procedure to clean the organ bath chambers and supports:
1. Fill the organ bath chamber to the edge with an 8% acetic acid solution and allow it to work for a few minutes
to dissolve calcium deposits and other salt build-up. Use a cotton-tipped applicator to mechanically clean all
chamber surfaces. Be careful not to apply too much force to the force transducer side mount.
2. Remove the acetic acid and wash the organ bath chamber and supports it several times with double distilled water.
3. If any kind of hydrophobic reagents has been used which might be difficult to remove using steps 1 and 2, then
try incubating the chamber and supports with 96% ethanol or a weak detergent solution (i.e. 0.1% triton-100).
4. To remove more resistant or toxic chemicals, incubate the organ bath chamber and supports with 1M HCl for
up to 1-2 minutes. In exceptional cases, incubate the chamber and supports with no stronger than a 3M HNO3
34
Summary of Contents for 820MO
Page 1: ...ORGAN BATH SYSTEM 820MO USER MANUAL VOL 1 2 ...
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