Thrust the excitation type selector (5) into the limiting position “B”. And place a white paper
on the stage. Turn the 10X objective in working. Then turn on the switch of the power supply box
(19) and a light shadow will fall down on the paper. Adjust the focusing rod for condenser (26) and
the focusing knob of the microscope to focus the shadow to be a dot.
4. Preparation of the sample:
The sample can not be too thick. Otherwise the judgment will be affected by the overlapping
cells or impurity.
Thickness of cover glass: 0.17mm;
Seal carrier: no fluorescence and no color;
Immersion oil: Special immersion oil for fluorescence device.
5. Fluorescent microscopy observation:
Turn off the halogen in the base and turn on the mercury lamp.
For the best fluorescence observation effect, it is necessary to close the field diaphragm in
the base or cover the collector with pressboard. Because the lenses in collector possibly
reflect ambient light to disturbance the fluorescent observation.
Push the flashboard B (6) in the middle position to pass the light with specific wavelengths.
According to the different demands, select the different spectrum range:
Pull the selector (5) out to the limiting position for G (green light) exciting state;
Push the selector (5) into the limiting position for B (blue light) exciting state;
Put the selector (5) in the middle position for general biological microscopy observation with
ordinary transmission light;
When the fluorescent microscopy observation is paused, please push the flashboard B (6) to the
left limiting position to stop the stimulating light and reduce the fluorescence quenching.
6. General biological microscopy observation:
Turn off the switch (17) of the power supply box (19) and push the selector (5) to the middle
position “O”. Turn on the switch in the base and adjust the brightness of the halogen lamp by
turning the plate (15) for general biological microscopy observation.
E) Use Scope of the Fluorescent Filter:
Filter
Use
Fluorescent Dye
Lighting
Color
Medicine Examination
Project
B
（
Blue
）
EX: 490
DM:
510
BA: 515
P1TC
；
Pyoktanin Yellow O
；
Alkalinity
Fluorescein
；
Acridine Orange
；
Fluorescein Sodium;
Tetracyclin Sodium;
Green,
Yellow
Orange;
Red
Fluorescence
Immune
Body;
Cell
Nucleus
of
the
Tubercidin Bacillus
；
Cancer Cell
、
Red Blood Cell
Protozoan
Venereal Diseases Series
G
（
Green
）
EX: 545
DM:
580
BA: 590
Luo Daming;
Luo Daming B200;
Acridine Orange
Orange-red
Fluorescence immune body
*DNA
P.5
