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OPERATION MANUAL 

 

EDGE

 Integrated Electrophoresis System

4

Specifications:

•   LED Wavelength: 470 nm +/- 10 nM

•   LED Life: 50,000 hours

•   Input voltage: 100-240V AC, 50/60 hz

•   Output voltage: Selectable at 100V or 150V DC

•   Dimensions: 17 cm (w) x 17 cm (l) x 15 cm (h)

•   Operating conditions: 4 °C to 37 °C, 

 

75% maximum humidity 

Guarantee:

The unit is guaranteed against any defect in material or 

workmanship for three years. The warranty period is from 

the date of receipt, and within this period all defective 

parts will be replaced free of charge provided that the 

defect is not the result of misuse, accident or negligence. 

Servicing under this guarantee should be obtained from 

EDVOTEK®. 

Notwithstanding, the description and specification(s) of the 

units contained in the User’s Manual, EDVOTEK® hereby 

reserves the right to make such changes as it sees fit to the 

units or to any component of the units. This Manual has 

been prepared solely for the convenience of EDVOTEK® 

customers and nothing in this Instruction Book shall be 

taken as a warranty, condition or representation concern-

ing the description, merchantability, fitness for purpose or 

otherwise of the units or components.

Contact Information:

For technical, sales or servicing information, contact: 

EDVOTEK, Inc.,

1121 5th Street NW 

Washington, DC 20001 

1.800.EDVOTEK (1.800.338.6835)

Fax: 202.370.1501

info@edvotek.com •  www.edvotek.com

Troubleshooting:

The EDGE™ does not power on:

• 

Check that the power cord is fully plugged into the 

EDGE™ base and that the cord and outlet are func-

tional.

• 

Confirm that the power switch on the rear of the 

EDGE™ base is turned on.

• 

Inspect the fuse and replace if needed. The fuse is 

located directly under the power plug on the rear of 

the EDGE.

The START/STOP indicator LED does not stay lit and/or 

the experiment does not run:

• 

Ensure that the lid is fully closed.

• 

Press firmly on the electrophoresis tank to ensure 

that it is firmly seated and that both electrodes are 

making contact with the base.

• 

Check that the correct buffer has been used and that 

the gel is fully submerged in buffer.

• 

Ensure that the timer has not reached zero. Increase 

the remaining time and try running the experiment 

again.

The gel tray will not fit into the chamber:

• 

The tray is designed to fit only one way. Rotate the 

tray 180˚ and try again.

• 

The EDGE™ uses custom 10 x 7 cm trays that differ 

from existing Edvotek electrophoresis accessories. 

Please ensure that you are using the proper electro-

phoresis trays.

No DNA bands are present on the gel:

• 

Check that the blue light is turned on by pressing the 

paddle switch

• 

Ensure that SYBR® Safe, or comparable blue 

light-compatible, DNA stain was added to the gel. 

Consider a post-electrophoresis stain like EDVOTEK® 

FlashBlue™ stain.

• 

Verify that the gel has run properly. This can often be 

confirmed by the migration of a loading dye included 

in the experimental samples.

The gel has run crooked, or the DNA samples are at an 

angle:

• 

Ensure that the gel tray is aligned properly in the 

buffer chamber. The tray should sit flush against the 

bottom of the chamber and against one wall of the 

chamber to ensure it is straight.

• 

Check that the electrodes are intact and that there is 

no visible corrosion on either electrode.

Power Switch

Fuse

Summary of Contents for EDGE

Page 1: ...7 cm gel tray with two dual sided combs for up to 16 samples per gel Flexible gel volumes 30 50 mL Built in timer 1 99 minutes Fan and ventilation system minimizes condensation during experiments Convenient hinged lid contains dual automatic power cutoff switches for safety Stainless steel and platinum electrodes are compatible with all standard electrophoresis buffers 17 x 17 x 15 cm Voltage Sele...

Page 2: ...ned for safety and convenience A hinged lid and dual safety cutoff switches help to prevent im proper use and minimize the risk of electric shock The blue light transilluminator allows users to monitor DNA migration throughout the experiment using SYBR Safe or similar stains without the hazards associated with UV light 1 2 3 A built in power supply allows users to select between 100V and 150V prod...

Page 3: ...ophoresis chamber should sit flush against the bottom of the EDGE base unit 2 COVER the gel with 1x electrophoresis buffer being careful to not overfill the chamber For best results the buffer should be approximately 0 5 cm above the surface of the gel Ensure that the gel tray is aligned in the center of the chamber 3 LOAD the DNA samples into the wells according to the experiment protocol 4 LOWER...

Page 4: ... and outlet are func tional Confirm that the power switch on the rear of the EDGE base is turned on Inspect the fuse and replace if needed The fuse is located directly under the power plug on the rear of the EDGE The START STOP indicator LED does not stay lit and or the experiment does not run Ensure that the lid is fully closed Press firmly on the electrophoresis tank to ensure that it is firmly ...

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