56
Rev B 2022/03
Initial Start-Up & Routine Start-Up Procedure
CyBio Well vario
6.3.4
Precision Test (Capillary Head)
To perform precision testing, use the following procedure.
Preparation:
To wet an inserted capillary magazine, use process settings as follows:
Procedure:
Allow 8 seconds for taking in p-nitrophenol (in DMSO) by capillary force. Then dispense
this solution into a 384 well microplate with transparent bottom for 1 second at a pressure
of 25 psi (1.72 bar). Fill the microplate up with 50
μ
l of 0.1 N NaOH solution (e. g. with a
pipetting head). Place the microplate onto a plate shaker and let it shake for 10 min at 700
to 1000 rpm Allow the plate then to rest for 10 min. Perform measurement of absorbance
at 405 nm after this rest time.
Rinse the microplate three times with water again after testing.
Result:
Typical values:
The p-nitrophenol solution filling should have a concentration of approximately 120
μ
Mol
for measurement. This is necessary to achieve an approximate absorption value of 0.6.
This requires initial concentration values as follows:
Precision testing may also be combined with fluorescence measurement.
Rinse cycles:
3x
Rinsing liquid:
Water
Aspiration
8 sec.
by capillary force
Submergence depth of capil-
laries for aspiration
4 mm
Dispensing
1 sec.
by pressure (25 psi, 1.72 bar)
Dispensing gap:
1.5 mm
TIP
This gap between the capillaries and the microplate must be kept in all cases. This pre-
vents pressure-dispensed liquid from spurting out.
Variation coefficient: (CV)
< 10 %
Capillary magazine
CV
25 nl
< 9 %
100 nl
< 3 %
250 nl
< 2 %
Capillary magazine
Initial concentration of
p-nitrophenol (in DMSO)
25 nl
240 mmol
50 nl
120 mmol
100 nl
60 mmol
250 nl
24 mmol
500 nl
12 mmol
750 nl
8 mmol
1000 nl
6 mmol
Summary of Contents for Analytik Jena CyBio Well vario
Page 1: ...User Manual CyBio Well vario ...
Page 3: ...Rev B 2022 03 III CyBio Well vario ...
Page 5: ...Rev B 2022 03 V CyBio Well vario ...
Page 6: ...VI Rev B 2022 03 CyBio Well vario ...
Page 7: ...Rev B 2022 03 VII CyBio Well vario ...
Page 8: ...VIII Rev B 2022 03 CyBio Well vario ...
Page 12: ...XII Rev B 2022 03 CyBio Well vario ...
Page 16: ...XVI Rev B 2022 03 CyBio Well vario ...
Page 26: ...10 Rev B 2022 03 Technical Specifications CyBio Well vario ...
Page 62: ...46 Rev B 2022 03 Technical Description CyBio Well vario ...
Page 118: ...102 Rev B 2022 03 Troubleshooting CyBio Well vario ...
Page 126: ...110 Rev B 2022 03 Decommissioning CyBio Well vario ...
Page 130: ...114 Rev B 2022 03 Accessories Spare Parts CyBio Well vario ...
Page 132: ...116 Rev B 2022 03 Waste Disposal CyBio Well vario ...
Page 142: ...126 Rev B 2022 03 CyBio Well vario ...
