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Operation:
Setting up the illumination
For optimum effect in contrast and resolution one should follow the below procedure:
Place a specimen on the object stage and focus using the 4x objective, with a fully opened iris diaphragm.
Turn light intensity to lowest position, then look through the eyepiece(s) and turn up to
comfortable intensity level
Turn the condenser in the highest position(for phase contrast models, please set condenser to
bright field position).
Close the iris diaphragm, until it is just visible on the edge of the field of view.
The microscope is properly set for use with the 4x objective. For each other magnification in bright field use
this procedure should be repeated to ensure the best balance between contrast and resolution. Phase
contrast use will be explained later in this manual.
Place the specimen slide
1.
Push the arm of the specimen holder backwards.
2.
Release the arm slowly clamping the slide with the cover glass facing up.
3.
Rotating the X and Y-axis knob will move the specimen to the center for alignment with the
center of the objective.
Focusing and slide protection
1.
Select the objective 4x to the optical path.
2.
Rotate the position screw to top, observe the right eyepiece with right eye. Rotate the
coarse focusing knob until the image appears.
3.
Rotate the fine focusing knob for detailed focusing
4.
When focused with S100x objective, lock the slide protection handle. The slide protection
handle protects the slide by limiting the travel of the table. This way the objectives will not
touch or break your slides.
Adjusting the focusing tension
The iScope series microscope focusing knobs can be adjusted for tension. You can set it from light
to heavy according your own preference. Please note that when the specimen leaves the focus
plane after focusing or the stage declines itself, the tension should be set higher. To tighten the
focusing arm (more heavy), rotate the tension adjustment ring according to the arrowhead
pointed; to loosen it, please turn it in the reverse direction.
The interpupillary distance
