up and make sure it springs back to the center. Move the storage block
back down again to the cartridges remain submerged. Top up the LN2.
30.
Repeat for all other positions. If a cartridge is present on the holder
(Cartridge button Yellow) allow one free position to unload it.
Transferring the Selection Rod to the Microscope
31.
Once all positions are loaded make sure V2 and V3 are closed and turn on
the scroll pump.
32.
Open V3 and wait for the pressure to fall to below 8x10
-‐2
Torr.
33.
Retract the storage block inside the Selection Rod and close VC.
34.
Open V2 and wait for the pressure to fall to below 8x10
-‐2
Torr.
35.
On the microscope remove the airlock cover by unclamping the two
clamps and wipe the 0-‐ring with lens paper.
36.
Close VA.
37.
Close V3.
38.
Open V4 to vent and bring the interface between VC and VA to
atmosphere.
39.
Unclamp the Selection Rod and swing to the right to undock from the
serial port. Lift up to un-‐hinge.
40.
Attach the selection Rod to the airlock hinges and swing in to dock. Secure
the top clamp first followed by the bottom clamp.
41.
Clock on Pump Airlock.
42.
V43 will open followed by V41. Once P10 gets to Log 36 the message to
open VA will appear.
43.
Pull VA to open and wait for the pins to pop in.
44.
A prompt will shortly follow asking to open VB.
45.
Pull VB to open and wait for the pins to pop in. The Selection Rod is now
connected to the Microscope vacuum. Turn the Turbo off and wait for the
Column vacuum to fall below Log 15.
Removing a cartridge from the holder
46.
Move the insertion Rod to the first stop, the checking position and check
in the airlock mirror that a cartridge is not present. If a cartridge is
present follow steps 50-‐53.
47.
Retract the Insertion Rod and click on Cartridge. This will move the stage
to the default exchange XYZ coordinates and unlock the solenoid safety
pin.
48.
Move the Insertion Rod slowly inwards to the final stop and ensure the
silver button pops up. Once locked double check by gently trying to move
the insertion rod to ensure it is locked in.
49.
Unscrew the specimen holder from the cartridge by turning the white
knob anti-‐clockwise until the red band is clearly visible. The Cartridge
button should now be grey.
Summary of Contents for Tecnai F30 Polara
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