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Operation

FEI Tecnai F30 Polara, Operation

The Operation Manual for the cutting-edge FEI Tecnai F30 Polara electron microscope can be downloaded for free from 88.208.23.73:8080. This comprehensive user manual provides detailed instructions and insights on operating this advanced equipment, allowing users to achieve optimal performance and obtain exceptional results in their research and analysis.

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Summary of Contents for Tecnai F30 Polara

Page 1: ...ur and should not go below 20 5 Use the metal funnel to top up the Insertion Rod dewar This dewar can last up to 10 hours If the Insertion Rod warms up the vacuum deteriorates and the HT may switch off When collecting with EPU a LN2 top up at 11pm will ensure it stays cold until 9am the next day 6 Check the temperatures of the different channels on the display 1 Helium cryo shield 2 Nitrogen cryo ...

Page 2: ...ition should be accessible 18 Remove old grids and C clips from the cartridges using 5 tweezers 19 Record the bottom and top cartridge identifier digits on the notepad 20 Begin cooling the transfer chamber initially without the lid and then through the side funnel to avoid condensation inside Continue cooling the chamber and keeping the LN2 level just above the cartridge loading area 21 After a co...

Page 3: ... to dock Secure the top clamp first followed by the bottom clamp 41 Clock on Pump Airlock 42 V43 will open followed by V41 Once P10 gets to Log 36 the message to open VA will appear 43 Pull VA to open and wait for the pins to pop in 44 A prompt will shortly follow asking to open VB 45 Pull VB to open and wait for the pins to pop in The Selection Rod is now connected to the Microscope vacuum Turn t...

Page 4: ...rds to the final stop and ensure the silver button pops up Once locked double check by gently trying to move the insertion rod to ensure it is locked in 61 On the holder push in the white knob until the red stripe disappears and then screw clockwise one complete turn to capture the threads on the cartridge Continue turning until finger tight DO NOT OVERTIGHTEN The Cartridge button should turn Yell...

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Page 6: ...Spirits one session is up to half a day 4 hours for negative stain and up to a full day 8 hours for cryo EM A half day is for example 9am 1pm 1pm 5pm or 5pm 9pm A whole day would be 9am 5pm You can of course book less time if you don t need the full 4 or 8 hour slot The actual booking times are also flexible e g 10 12pm Please note that evenings and weekends count the same on the Spirits If on the...

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