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Fein 

8

Microscope Operation

Adjust the Focusing Tension:

If it becomes hard to move the coarse focus 

knob, or the stage drifts and the sample falls 

out of focus the tension needs to be adjusted. 

Tighten the tension by adjusting the tension 

adjustment ring (1) in the direction of the 

arrow shown at right. Loosen the tension by 

rotating the tension adjustment ring in the 

opposite direction. 

Adjusting the Interpupillary Distance:

When observing with both eyes, adjust 

the eyepieces (2) to fit your personal 

interpupillary distance. The scale between 

the eyepieces (1) notes the interpupillary 

distance setting. It’s a good idea to note 

this number when working in a setting 

where multiple people are using the same 

instrument. This allows you to easily reset 

your interpupillary distance. 

1

Aperture Diaphragm:

The aperture diaphragm dictates the 

numerical aperture (NA) of the illumination. 

The best resolution, contrast, and depth 

of field is obtained when the NA of the 

illumination matches with the NA of the 

objective. Rotate the diaphragm adjustment 

ring (1) so the pointer matches up with the 

inscription of the objective being used.

1

1

2

1

2

Summary of Contents for RB20-HD

Page 1: ...Fein RB20 Biological Microscope User s Manual info feinoptic com...

Page 2: ...Fein Microscope Components Eyepieces Condenser Objective Lens 1 Coarse Adjustment Fine Adjustment Tension Adjustment Microscope Frame Carrying Handle Condenser Adjustment Knob...

Page 3: ...epiece Mechanical Stage Fein Microscope Components 2 Lock Screw Fixed Koehler Illumination Focus Arm Trinocular Head Vertical Adjustment Knob Horizontal Adjustment Knob Power Switch Light Adjustment K...

Page 4: ...ck 1 and the frame at the front 2 as shown in the image at right Set the power switch to off O before replacing a bulb or fuse and wait until the lamp is cool The power switch is located on the side o...

Page 5: ...4 Fein Microscope Assembly Follow the numbers below when assembling the microscope Eyepiece Condenser Objectives Power Cord 1 2 3 4...

Page 6: ...il the condenser is aligned with the base and rotate the condenser to make the handle face forward Tighten the condenser lock screw 2 of condenser then raise the condenser with the up down knob to the...

Page 7: ...ntil the eyepiece is flush with the eyepiece tube Tighten the eyepiece with M2 5 inner hexagon lock screw 3 1 3 2 Assemble the Eyepiece Take off the eyepiece tube cover 1 Insert the eyepiece 2 into th...

Page 8: ...wly move the specimen holder lever back into place This will secure the specimen slide Rotate the X and Y axis knobs 3 on the stage until the center of the specimen slide is aligned with the objective...

Page 9: ...e eyepieces 2 to fit your personal interpupillary distance The scale between the eyepieces 1 notes the interpupillary distance setting It s a good idea to note this number when working in a setting wh...

Page 10: ...r use wipe the lens with a tissue moistened with microscope cleaning solution Using Filters Filters can increase the contrast in microscopy images The filter sits on top of the light as shown at the r...

Page 11: ...e microscope is not in use the power cord can be placed in the storage box Press the position 1 of the storage box cover with thumb When you hear a click push up and put the power cord into the storag...

Page 12: ...over glass The cover glass is not standard Use a standard cover glass with thickness of 0 17mm The slide is upside down Adjust the slide The immersion oil has accumulated on the dry objective Clean th...

Page 13: ...xed correctly Adjust it The movable specimen holder is not fixed properly Adjust it The image moves obviously when touching the stage The stage is fastened incorrectly Fasten the stage correctly The L...

Page 14: ...rrying handle side is down Locate the 4 rubber feet on the underside of the microscope Remove the rubber feet exposing the 4 screws securing the bottom plate of the microscope Gently lay microscope on...

Page 15: ...th all 4 screws removed carefully remove the bottom plate exposing the internal components of the microscope Locate the 2 screws securing the LED bulb in place Using a screw driver remove the 2 screws...

Page 16: ...the LED bulb replaced position the bottom of the microscope plate back onto the microscope frame Using a screw driver secure the bottom plate with the 4 set screws Place the 4 rubber feet over the to...

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