Full Phase Contrast / Polarization Assembly:
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Page 11
Phase Contrast Condenser Assembly:
Install phase contrast objective lenses.
Insert the condenser into the holder and tighten set
screw.
Rotate the phase adjustment ring (2) to “BF”
(brightfield) until it clicks into place.
Focus on a sample to ensure condenser is installed
straight.
Centering the Phase Condenser:
Slide the aperture diaphragm lever (1) to the furthest
left position while the aperture diaphragm is fully
open.
Place a specimen on the stage and focus.
Remove one eyepiece and insert the phase
centering telescope.
Set the phase adjustment ring (2) to correspond with
the phase objective being used (example: PH10x
with 10x objective).
Adjust the centering telescope to get a clear image
of the phase ring and halo in the field of view.
Center the rings (4) and (5) by adjusting the
centering screws (3) on the phase condenser.
If the phase condenser is not centered prior to use
phase contrast microscopy observation will not be
possible.
Polarization Assembly:
Simple polarization includes both a polarizer (3) and
analyzer (2).
Remove analyzer dust cap (1) from microscope and
insert analyzer facing up.
Place the polarizer (3) on the lamphouse (4).
Rotate the polarizer when looking through the
microscope.