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Page 4

O

RGANISM 

A

PPLICATOR

:

  

 

 
An atomizing humidifier spray nozzle mounted at the center of the test duct intake was 
used to distribute the organism into the air stream.  The application flow rate was 0.45 
gallons per hour. 
 
UV D

EVICE

:

 

 

 
A Field Controls UV-Aire air purifier model UV-18 was mounted onto the center of the 
side of the test duct 6 feet from the exit end of the chamber.  The lamp is a UVC 
germicidal lamp (non ozone producing) 18 inches long with a UV output rating of 73 

µ

W/cm

2

 at 1 meter from the lamp. 

 
A

IR 

S

AMPLING 

M

ETHOD

:   

 
An Andersen N6 single stage “bioaerosal” sampler was used to take the air samples and 
distribute the sampled air onto agar medium.  The test medium was Tryptic Soy Agar 
from PathCon, Inc.  The air sampling pump airflow rate was 1 CFM.   

The Anderson sampler method requires corrections to the actual colony counts on the 
plates.  This provides a more accurate  measure of the bacteria per cubic foot of the 
sample air.  In the following tables, the 

Serratia marcescens

 Positive Hole Count values 

are the actual plate counts and the Corrected Particle Count values are corrected value 
based on Anderson correction tables. 

Test Apparatus 

 

Figure 1 

 

Summary of Contents for 4291UV-AIR

Page 1: ...al Assays Sanitary Environmental Microbiology Bernard E Kane Ph D 1706 Canterbury Rd Greenville NC 27858 Ph 252 355 6789 Oysterdoctor aol com Efficiency Of Bacterial Disinfection By A Duct Mounted UV Aire Air Purifier For ...

Page 2: ...Testing Structure 3 Testing Airflow Rate 3 Organism Applicator 4 UV Device 4 Air Sampling Method 4 Test Apparatus 4 Testing Procedure 5 Tables 6 7 Table 1 Control Data 6 Table 2 UV 18 Test Data Results 6 Table 3 UV 18X Test Data Results 7 Conclusion 8 ...

Page 3: ...g plants and large commercial HVAC systems ORGANISM Serratia marcescens ATCC 14756 was chosen as the test bacterium The distinctive red colonies made it easy to evaluate from any background organisms A raw test suspension of the organism of approximately 95 000 CFU ml was used As dispersed into the test system this suspension yielded bacterial counts of 269 CFU ft3 500 ft min airflow and 107 5 CFU...

Page 4: ... cm2 at 1 meter from the lamp AIR SAMPLING METHOD An Andersen N6 single stage bioaerosal sampler was used to take the air samples and distribute the sampled air onto agar medium The test medium was Tryptic Soy Agar from PathCon Inc The air sampling pump airflow rate was 1 CFM The Anderson sampler method requires corrections to the actual colony counts on the plates This provides a more accurate me...

Page 5: ... through the spray nozzle mounted in the center of the duct inlet Five air samples were taken for each of the test velocities at short intervals typically to 2 minutes This produced a large sample volume of air and reduced the levels of back ground bacteria and molds counts The plate counts colony forming units or CFU for each of the five tests were totaled and divided by the total test volume of ...

Page 6: ... 1000 92 103 Total min 8 Total Corrected Particle counts 860 107 50 Table 2 UV 18 Test data and results testing with lamp on Sample Number Air Sampling Duration min Airflow Velocity fpm Serratia marcescens Positive hole count Corrected Particle Counts CFU FT 3 of air count min Survival CFU Control Log Reduction Effective 1 1 1000 30 31 2 1 1000 32 33 3 3 1000 88 99 4 6 1000 145 180 Total min 11 To...

Page 7: ...article Counts CFU FT 3 of air count min Survival CFU Control Log Reduction Effective 1 2 5 1000 21 22 2 2 5 1000 27 28 3 2 5 1000 28 29 4 5 1000 48 51 Total min 12 5 Total Corrected Particle Counts 130 10 40 9 67 1 01 90 33 Control 107 50 1 5 500 8 8 2 5 500 10 10 3 5 500 17 17 4 10 500 28 29 Total min 25 Total Corrected Particle Counts 64 2 56 0 95 2 02 99 05 Control 269 00 ...

Page 8: ...ergy level At the higher velocity the lamp still reduced the bacterial levels by at least 71 at a 50 decrease in the exposure time Since the reduction efficiency is based on lamp UV output and exposure time the assumption can be made that decreasing the exposure time to the UV light is similar to testing an organism that requires a higher UV energy requirement to kill the bacteria The log reductio...

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