F
LEXCELL
®
I
NTERNATIONAL
C
ORPORATION
HT
C
ELL
S
EEDER
U
SER
I
NSTRUCTIONS
P
AGE
1
OF
3
I
NTRODUCTION
The HT Cell Seeder
™
(
Figure 1
) confines cells during plating to the area of the HT BioFlex
®
membrane
that is directly over the Loading Station
™
. Thus, it prevents cells from being subjected to undefined
strains when using the 24-well equibiaxial Loading Stations
™
during strain application (
Figure 2
). The
HT Cell Seeder
™
is inserted into the 24-well HT Baseplate (
Figure 3
), and the HT BioFlex
®
plate plus
the gasket is placed on top of the HT Cell Seeder
™
. The HT Cell Seeder
™
is only required for seeding
cells onto the membrane for a minimal period of time. Afterwards, cell feedings and experiments can be
conducted as normal.
Figure 1. 24-well HT Cell Seeders
™
.
Figure 2. A) A cylindrical loading post used when applying equibiaxial strain under the well of an HT
BioFlex
®
plate. Cells seeded on the membrane in areas outside of the loading post will be subject to
undefined strains. B) The results of using an HT Cell Seeder
™
when plating cells to confine them to the
area directly above the loading post (blue = Crystal Violet stain; bar = 1 mm).
A
B
