F
LEXCELL
®
I
NTERNATIONAL
C
ORPORATION
HT
C
ELL
S
EEDER
U
SER
M
ANUAL
P
AGE
2
OF
3
Figure 3. HT Cell Seeders
™
in a 24-well HT Baseplate.
I
NSTRUCTIONS
1.
Insert the HT Cell Seeder
™
into a 24-well HT Baseplate, similar to how the cylindrical loading
stations are placed in the baseplate.
2.
Place an HT BioFlex
®
plate and its respective gasket over the HT Cell Seeder
™
, ensuring that the
wells in the plate align with the posts on the HT Cell Seeder
™
.
3.
Using the FX-5000
™
Tension System, create and start a regimen with the following settings:
SHAPE: Static
MIN: 0.0
MAX: 8.0
FREQ: 1 Hz (this value must still be entered even though the regimen is static)
DURATION: Equal to the seeding time (see step 5 below) plus the time needed to plate
the cells into the well.
PLATFORM: HT 24-Well Plate (Cylindrical LS)
NOTE: The membrane may be subjected to strains up to 1-2% (10 to 20 microns per 1 millimeter) when
seeding, due to the inherent strain caused when using the HT Cell Seeder
™
.
4.
The recommended suspension volume when using an HT Cell Seeder
™
for an individual well is
175 µL. This volume is large enough to allow uniform distribution of the media-cell suspension
within the well when dispensing with a 1000 µL micropipette (
Figure 4
). Also, this volume is
small enough to prevent the media-cell suspension from spilling out of the HT Cell Seeder
™
when handling the baseplate.
