Gatan ORIUS SC 200D, User Manual

Page 1: ...ORIUS SC 200D CCD Camera User s Guide Gatan Inc 5794 W Las Positas Blvd Pleasanton CA 94588 USA Tel 925 463 0200 FAX 925 463 0204 September 2008 833 MANUAL Revision 3...

Page 2: ...rizes the features of the ORIUS family cameras Chapter 3 Getting Started shows the first steps necessary to start the DigitalMicrograph software and prepare for the acquisition of an image Gain Normal...

Page 3: ...s any implied warranties of merchantability or fitness for a particular purpose Gatan Inc further reserves the right to revise this manual and to make changes to its contents at any time without oblig...

Page 4: ...ct by electronic mail Gatan USA West Coast Tel 925 463 0200 Fax 925 463 0204 Gatan USA East Coast Tel 724 776 5260 Fax 724 776 3360 Gatan Germany Tel 49 89 358084 0 Fax 49 89 358084 77 Gatan UK Tel 44...

Page 5: ...2 5 2 5 1 Low magnification 2 6 2 5 2 High magnification 2 8 3 IMAGE ACQUISITION 3 1 3 1 VIEW 3 1 3 2 IMAGE NOTES 3 4 3 3 TEM SESSION INFORMATION GLOBAL INFO 3 4 3 4 IMAGE ACQUISITION 3 5 3 5 IMAGE SA...

Page 6: ...REQUIREMENTS I A2 DESCRIPTION II A3 INSTALLATION III A3 4 Electrical connections VI APPENDIX 2 VII ENVIRONMENTAL SPECIFICATIONS AND SAFETY REQUIREMENTS VII TEM REQUIREMENTS VIII Operation requirement...

Page 7: ...FIGURE 2 9 CHOOSE YES TO COMPLETE THE CALIBRATION 2 8 FIGURE 2 10 MAGNIFICATION TABLE 2 8 FIGURE 2 11 HIGH RESOLUTION IMAGE OF A CRYSTALLINE SAMPLE 2 9 FIGURE 2 12 DIFFRACTOGRAM 2 9 FIGURE 2 13 HIGH...

Page 8: ...or require exposures of less than 50ms you have too much beam On a thin film for example carbon 99 9 or more of the beam will be in the center spot This could be as much as 100nA on a typical TEM Red...

Page 9: ...ORIUS 833 CCD Camera User s Guide Series Rev 3 viii Any condition that places a very bright spot in a single place on the scintillator and shows streaking in the background of the image...

Page 10: ...cation It is important to wait for the camera controller to boot fully before starting DM Under the camera menu of DM set or confirm the camera is set to cool to 10C Check the TEM session information...

Page 11: ...1 Acquire your data DigitalMicrograph allows you to acquire data from many different sources such as CCD cameras EELS systems or imaging filters Your data may be an image a spectrum or something tota...

Page 12: ...ported from other applications DigitalMicrograph makes it easy to arrange multiple images and other annotations on a page to produce the layout you desire 6 Archive your data At each part of this proc...

Page 13: ...Power Ready and Camera lit green Turn the computer on if it is not on yet and double click in the DigitalMicrograph icon to launch the application Set the camera to cool by picking the option Temperat...

Page 14: ...Camera configuration If the camera is already configured by a Gatan service engineer this section can be ignored Before starting acquiring images it is necessary to configure the camera This process i...

Page 15: ...r this camera 2 3 Dark Correction After the camera has cooled to its operating temperature of 10 C a calibration of the bias or thermal counts is required This is often referred to as the dark image Q...

Page 16: ...te slight beam profile differences on the TEM when magnification is changed When your application need requires large ranges in magnification the single gain reference image might not be sufficient an...

Page 17: ...n spacing At High magnifications Use graphite or any crystalline samples having known lattice spacing Use the FFT method It is very important to make sure DigitalMicrograph software correctly reads th...

Page 18: ...w Figure 2 4 choose Select the checkbox Ask User next to Magnification Pull down the menu and select Always Click OK 2 5 1 Low magnification Record an image of a cross grating sample Figure 2 6 Figure...

Page 19: ...K on the Calibrate image window Enter the correct distance for the selected feature for example 10 line pairs of cross grating sample where the distance 10 x 0 463 m in the Calibration window and sele...

Page 20: ...nal microscope magnification and the calibrated value To view the magnification table Select Calibrations from the Microscope menu Figure 2 5 Figure 2 10 Magnification table The first number entry rep...

Page 21: ...ctogram under the Microscope menu Figure 2 5 A diffractogram will be calculated after placing a FFT ROI on the image Figure 2 12 Figure 2 12 Diffractogram Follow the on screen instructions Figure 2 13...

Page 22: ...ev 3 2 10 Press OK to specify the reciprocal unit and the d spacing in the corresponding real units in the next window that appears Figure 2 14 Figure 2 13 High magnification calibration instructions...

Page 23: ...CCD field of view This is to allow the user to quickly conduct a sample search using the CCD camera On this camera setting of 4x binning 0 032 seconds exposure and quality mode set to Fast will give...

Page 24: ...other feature to aid in diffraction pattern viewing and acquisition is a software setting to remove streaking artifact from the image Streaking can occur normally when imaging intense transmitted beam...

Page 25: ...ORIUS 833 User s Guide Series Rev 3 3 3 Figure 3 1 Image window Figure 3 2 Focus Loupe The acquisition can be stopped by Pressing Stop View or Pressing the Spacebar...

Page 26: ...mage file Figure 3 3a Image Info 3 3 TEM Session Information Global Info Before acquiring any images it is recommended to enter the correct information about the sample operator TEM name etc On the Im...

Page 27: ...esolution CCD image Streaking correction can also be selected in the Setup Advanced section for use during acquisition of diffraction patterns Figure 3 4 Camera Acquire window 3 5 Image Saving and Pri...

Page 28: ...merous image formats are supported and a unique feature of batch conversion from Gatan dm3 format to other formats is available Figure 3 4 Toolbar Icons 3 5 1 Save Numbered and file storage path Click...

Page 29: ...Global Info dialog under Databar you can choose Page mode and Image mode in the left pane Select Page Mode to view the checkbox items that can be selected in the right pane Any items selected will be...

Page 30: ...ul to add the name of your institution ID s or the name of your TEM lab Figure 3 7 Figure 3 7 Custom Data 3 5 4 Report Print Preview The Print Preview button the one with a page and a magnifier on the...

Page 31: ...mize the layout and selection of parameters on the page for example using pointer to move the text around and then save the setting as a report template This is done by navigating via Edit Databar Sav...

Page 32: ...ect the performance of the camera so their description is offered here for the user to understand in case of any inadvertent changes made Figure 3 11 Figure 3 12a Camera View setup Figure 3 12b Camera...

Page 33: ...em should protect itself against this but it is not recommended to test this functionality Check the flow of cooling water periodically If the flow rate of the cooling water deviates significantly fro...

Page 34: ...be damaged Rather damage can result from prolonged exposure of the scintillator to a very high beam intensity above 15 000 counts in binned by 1 mode exposure of 10ms If you have no saturation with a...

Page 35: ...ul 1 There is no image on the CCD what should I do Check the Camera View floating window to see if the Camera Inserted box is checked camera inserted Check the camera really inserted and is not stuck...

Page 36: ...ece of debris on the sample is seen This will affect the histogram and appearance in compensating for the large amount of black pixels seen 8 How long is the lifetime of the CCD None of Gatan s CCDs h...

Page 37: ...ms are required Windows XP Professional Minimum 512 MB RAM 1GB RAM preferred 200GB Hard drive or better Pentium 4 1Ghz or higher Available microscope bottom port or 35mm port If model is available Wat...

Page 38: ...gnal chain light generation in the scintillator optical coupling by the lens image detection by the CCD pixel and also by cooling and low noise readout Resolution is achieved by minimizing light scatt...

Page 39: ...trained by Gatan then you must not attempt to install the camera Installation by an untrained person will invalidate the warrantee and may cause damage to the equipment and injury to the installer Bel...

Page 40: ...ORIUS 833 User s Guide Series Rev 3 IV Figure A4 Camera Pneumatic Interface Details...

Page 41: ...ORIUS 833 User s Guide Series Rev 3 V Figure A5 Water lines...

Page 42: ...ORIUS 833 User s Guide Series Rev 3 VI A3 4 Electrical connections The diagram in Figure A6 shows the electrical connections in the system Figure A6 Electrical diagram...

Page 43: ...ORIUS 833 User s Guide Series Rev 3 VII Appendix 2 Environmental specifications and safety requirements...

Page 44: ...ration Care should be taken not to restrict the supply Water cooling 0 2 0 5 l min at 15 to 25 C 3kgf cm2 or 3 bar inlet pressure no restriction on outlet flow Pressurized air 60 90 psi or 4 6 kgf cm2...

Page 45: ...ORIUS User s Guide Series Rev 3 IX Appendix 3 Camera specifications...

Page 46: ...2 pixels single port Dynamic Range 2500 1 maximum noise 4096 grey values Gain uniformity 10 r m s in uncorrected images Maximum column defects Total 10 No adjacent column defects Exposure settings 100...

Page 47: ...ORIUS 833 User s Guide Series Rev 3 XI Appendix 4 CE certification...

Page 48: ...ORIUS 833 User s Guide Series Rev 3 XII...

Page 49: ...DigitalMicrograph i ii 1 1 2 1 2 2 2 5 E exposure time 4 2 4 3 4 4 F FFT 2 5 film 2 5 4 2 4 3 Focus Loupe 1 2 3 2 3 3 Focus mode 3 2 G gain reference 1 1 2 4 4 4 4 5 ghost image 4 5 Global Info 1 1 3...

Page 50: ...on 2 10 3 1 3 2 3 5 II retraction II S saturation 4 2 Saving 1 2 2 1 3 4 3 5 scintillator 4 2 4 3 II Search mode 1 2 3 1 3 2 Set up 3 6 Standard i Start Acquire 1 2 3 5 Start View 1 2 3 2 T TEM 1 1 2...