3.5.3
Optical Densitometry
Introduction
For optical density (OD) measurements, pixel data are converted to OD values to obtain
a linear correlation between the output signal and the amount of sample. Each instrument
is factory calibrated using an OD step tablet. In this mode, basic settings are predeter-
mined and cannot be changed.
OD measurements can only be made in the lower tray position using Trans il-
lumination.
Note:
Take an OD image
To take an OD colorimetric image, use the following steps.
Action
Step
On the Home screen, tap Colorimetric image capture.
1
Under 1.Select Exposure, tap OD measurement.
2
Under Advanced settings, change the settings for Capture area as required.
See
Section 3.3 Advanced settings, on page 41
for more information.
3
To save the capture settings for future use, tap Save method. In the popup
window, enter the Name of the method and tap Save.
4
Tap Start.
Note:
Both color and greyscale images for analysis are saved in the image file
folder.
5
Amersham ImageQuant 800 User Manual 29383813 AA
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3 Handle methods in ImageQuant 800 control software
3.5 Colorimetric image capture
3.5.3 Optical Densitometry
