8.1 Before you start
Introduction
Before starting a run, it is necessary to read and understand the information in this
section and to perform the checks listed below.
Checklist
Make sure that the system is correctly prepared. Check that:
•
the system is prepared according to chapter 6.
•
a suitable column has been selected for the application (consider target protein and
pressure range).
•
the buffer inlet tubing is immersed in correct buffer vessels (consider solution identity
and volume).
•
the waste tubing is immersed into appropriate waste vessels (consider vessel size
and vessel material).
•
no tubing is twisted and the flow path is free from leakage.
Hold, pause or stop the run
At the end of a method the run stops automatically. All pumps stop and the system
makes an acoustic end signal and displays End in the Run Log.
To interrupt a method during a run you may use the Hold, Pause or End icons in System
Control. A held or paused method run can be resumed by using the Continue icon. See
the instructions in the table below.
then...
If you want to...
click the Hold icon.
temporarily hold the method, with current
flow rate and valve positions sustained
click the Pause icon.
temporarily pause the method, and stop
all pumps
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Getting Started with ÄKTAavant and UNICORN 6 28-9440-69 AB
8 Run a method
8.1 Before you start
Summary of Contents for AKTA avant
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