Action
Step
Washing: Remove the Staining solution. Rinse three times in
distilled water for one minute each time.
5
Developing: Add developing solution and gently shake for
3 minutes. Transfer the gel to stopping solution when the
bands/spots have reached desired intensity.
Note:
If the background and and the bands are too strong, use a cold
transfer buffer and/or transfer the gel to stopping solution before
desired density has been reached.
6
Stopping: Gently shake the gel in stopping solution for
120 minutes.
7
Preserving: Add preserving solution and shake gently for
120 minutes.
8
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Amersham ECL Gel and Gel Box User Manual 28-9943-33 AC
5 Protocols
5.3 Post-staining of the gel
Summary of Contents for Amersham ECl Gel Box
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