GE Amersham Imager 600, Operating Instructions Manual

Page 1: ...Amersham Imager 600 Including 600 600 UV 600 QC 600 RGB Operating Instructions Original instructions ...

Page 2: ...ab 48 3 5 3 Description of the image view 52 3 5 4 Description of the analysis workflow 55 3 5 5 Description of settings views 59 3 6 Accessories 62 4 Installation 63 4 1 Site requirements 65 4 2 Instrument setup 66 4 3 System settings 67 4 3 1 Date and time 69 4 3 2 Focus 71 4 3 3 Instrument indicator 72 4 3 4 Service 74 4 4 Network 75 4 4 1 Network setup 79 4 4 2 Network access from instrument 8...

Page 3: ...tware installation and activation 180 6 1 1 Amersham Imager 600 Analysis Software installation 183 6 1 2 Amersham Imager 600 Analysis Software registration 187 6 2 Software operation 191 6 2 1 Lane creation 193 6 2 2 Background subtraction 198 6 2 3 Band detection 200 6 2 4 Molecular weight assignment 203 6 2 5 Normalization 204 6 2 6 Summary 207 7 Maintenance 208 7 1 Instrument 209 7 2 Accessorie...

Page 4: ...is Operating Instruction You should be acquainted with the use of general laboratory equipment and with handling of biological materials The instrument is installed by GE representatives About this chapter This chapter contains important user information description of safety notices regula tory information and a general description of the intended use of Amersham Imager 600 In this chapter See pa...

Page 5: ...camera system that produces digitial images of samples in gels or membranes in three different modes chemiluminescence colorimetric and fluorescence Amersham Imager 600 is intended for research use only and shall not be used in clinical procedures or for diagnostic purposes Safety notices This user documentation contains safety notices WARNING CAUTION and NOTICE concerning the safe use of the prod...

Page 6: ...used to indicate information that is important for trouble free and optimal use of the product Note A tip contains useful information that can improve or optimize your procedures Tip Typographical conventions Software items are identified in the text by bold italic text A colon separates menu levels thus File Open refers to the Open command in the File menu Hardware items are identified in the tex...

Page 7: ...ormity is available on request Title Directive Machinery Directive MD 2006 42 EC Electromagnetic Compatibility EMC Directive 2004 108 EC International Standards This product fulfills the requirements of the following standards Notes Description Standard EN 61010 1 lat est edition aligned with EN 61010 2 081 Safety requirements for electrical equipment for measurement con trol and laboratory use IE...

Page 8: ...essly approved by GE could void the user s authority to operate the equipment Note This equipment has been tested and found to comply with the limits for a Class A digital device pursuant to part 15 of the FCC Rules These limits are designed to provide rea sonable protection against harmful interference when the equipment is operated in a commercial environment This equipmentgenerates uses and can...

Page 9: ... II Waste Electrical and Electronic Equipment WEEE Directive 2012 19 EU Administration on the Control of Pollution Caused by Elec tronic Information Products China Restriction of Hazardous Substances RoHS ACPEIP Registration Evaluation Authorization and restriction of CHemicals REACH Regulation EC No 1907 2006 Regulatory compliance of connected equipment Any electrical equipment connected to Amers...

Page 10: ...mersham Imager 600 depending on configuration is equipped with some or all light sources in the table below Wavelength Light sources 460 nm Blue LED 520 nm Green LED 630 nm Red LED 470 nm to 635 nm White LED 312 nm Trans UV illuminator 10 Amersham Imager 600 Operating Instructions 29 0645 17 AD 1 Introduction 1 2 Regulatory information ...

Page 11: ...ger 600 instrument in addition to information on the safe disposal of the instrument In this chapter See page Section 12 2 1 Safety precautions 22 2 2 Labels 25 2 3 Emergency procedure 26 2 4 Recycling information 27 2 5 Declaration of Hazardous Substances DoHS Amersham Imager 600 Operating Instructions 29 0645 17 AD 11 2 Safety instructions ...

Page 12: ...an de scribed in the User Manual WARNING Use of this equipment in other ways than those specified in the user documentation may result in physical damage because of exposure to irradiated light or electric shock or injury from touching an operating part WARNING Do not use the instrument if smoke atypical noises or odors can be perceived or if the instrument becomes unusually hot as this may result...

Page 13: ...isconnect WARNING Do not block vents and ensure that they are kept free of dust and dirt Blockagecan causeoverheatingand malfunctioning To ensure adequate cooling ensure there is at least 40 cm of free space in front of the instrument and at least 10 cm on all other sides to walls or other equipment Personal protection WARNING Hazardous substances When using hazardous chemicals take all suitable p...

Page 14: ...ION Do not touch the UV unit after UV exposure There is a risk of skin burn Installation WARNING As the equipment is heavy contact a GE service person when moving or relocating the main body of the equipment If the equipment drops it may be broken or you may get seriously injured if it drops on your foot WARNING Power cord Only use power cords with approved plugs delivered or approved by GE WARNIN...

Page 15: ...nge AC power cord to inappropriate one WARNING Connect the power supply directly to a grounded wall power outlet The use of extension cords or multiple loads on one electrical outlet could result in fire and electric shock CAUTION Amersham Imager 600 must be secured before transport or relo cation Contact GE for instructions before transporting the instru ment NOTICE Do not connect telephone lines...

Page 16: ...ersonal Protective Equipment PPE during operation and maintenance of Amersham Imager 600 Protective eyewear Protective gloves Protective footware preferably with steel lining CAUTION Reagents used to prepare the sample should be used in accordance with the manufacturer instructions CAUTION Do not lean on the sample door CAUTION Use the handle to open or close the instrument door 16 Amersham Imager...

Page 17: ...cause damage to the instrument CAUTION Do not disable the interlock of the equipment as UV light can be emitted if the door is opened NOTICE Both the Amersham Imager 600 Control Software and Amersham Imager 600 Analysis Software do not analyze the image files other than those captured and generated by Amersham Imager 600 Analysis of files other than those captured on the Amersham Imager 600 may in...

Page 18: ...ay impair performance Simultaneous use can cause loss of data NOTICE Avoid connecting additional accessories such as computers monitors or keyboards to the instrument during operation as this can stop the exposure or cause loss of data NOTICE Do not turn off power during operation as this can cause loss of data and damage the Compact flash drive Only turn off power in an emergency situation NOTICE...

Page 19: ... system use a monitor with SVGA resolution With a low resolution monitor operation buttons cannot be seen NOTICE Remove all traces of acidic solutions to prevent corrosion in the equipment Maintenance WARNING Electrical shock hazard All instrument repairs or modifications should be performed by service personnel authorized by GE Do not open any covers or replace parts unless specifically stated in...

Page 20: ...el lining CAUTION Turn off the power switch before cleaning the inside of the instru ment CAUTION UV lamps in Amersham Imager configurations 600 UV 600 QC and 600 RGB contain mercury which must be recycled or disposed of in accordance with local regulations NOTICE Do not use abrasive cleaning materials such as a scouring pad for cleaning This may cause damage to the instrument NOTICE Dispose of th...

Page 21: ...erformingmaintenanceoftheequipment removethetablet computer There is risk of injury if it falls on your foot Amersham Imager 600 Operating Instructions 29 0645 17 AD 21 2 Safety instructions 2 1 Safety precautions ...

Page 22: ... Imager 600 system label The Amersham Imager 600 instrument serial number is found on the label located on the back of the instrument Symbols used on the labels The following symbols are found on the label on the instrument Meaning Label Warning Read the Operating Instruction before using the system Do not open any covers or replace parts unless specifically stated in the Operating Instruction 22 ...

Page 23: ...his symbol indicatesthat the product containshazardous materials in excess of the limits established by the Chinese standard SJ T11363 2006 Requirements for Concentration Limits for Certain Hazardous Substances in Electronics This symbol indicates that the instrument has been certified by a Nationally Recognized Testing Laboratory NTRL An NRTL is an organizationthattheOccupationalSafetyandHealthAd...

Page 24: ...iption on page 33 Note Description Symbol On Off switch Power switch I O I Power ON O Power OFF Connector for DVI D monitor DVI D USB port Ethernet port Caution hot surface 24 Amersham Imager 600 Operating Instructions 29 0645 17 AD 2 Safety instructions 2 2 Labels ...

Page 25: ...nnect the power cord from the wall socket WARNING Access to power switch and power cord with plug Do not block access to the power switch and power cord The power switch must always be easy to access The power cord with plug must always be easy to disconnect Amersham Imager 600 Operating Instructions 29 0645 17 AD 25 2 Safety instructions 2 3 Emergency procedure ...

Page 26: ...cled according to national and local environmental regulations Recycling of hazardous substance The lamps in Amersham Imager 600 configurations 600 QC 600 UV and 600 RGB contain mercury vapour These must be recyled or disposed of in a manner compliant with national and local environmental regulations Disposal of electrical components Waste of electrical and electronic equipment must not be dispose...

Page 27: ...od EFUP which indicates the period during which the toxic or hazardous substances or elements contained in electronic information products will not leak or mutate under normal operating con ditions so that the use of such electronic information products will not result in any severe environmental pollution any bodily injury or damage to any assets The unit of the period is Year In order to maintai...

Page 28: ...n Product 0 表示该有毒有害物质在该部件所有均质材料中的含量均在SJ T11363 2006标准规定的限 量要 求以下 X 表示该有毒有害物质至少在该部件的某一均质材料中的含量超出SJ T11363 2006标准规 定的限量要求 此表所列数据为发布时所能获得的最佳信息 0 Indicates that this toxic or hazardous substance contained in all of the homogeneous ma terials for this part is below the limit requirement in SJ T11363 2006 X Indicates that this toxic or hazardous substance contained in at least one of the homoge neous ma...

Page 29: ...changes to the software the screen captures in this manual may differ sligtly compared to the acutal software However the general functions are the same Note In this chapter See page Section 30 3 1 Introduction 31 3 2 Feature overview 32 3 3 Illustration of the instrument 33 3 4 Instrument hardware description 40 3 5 Instrument software description 59 3 6 Accessories Amersham Imager 600 Operating ...

Page 30: ... gels and on mem branes obtained by electrophoresis or western blotting separation methods The instru ment can capture images of chemiluminescent fluorescent and colorimetric samples depending on the system configuration and comes with analysis software The instrument can be used for research purposes in the academia and life sciences industry 30 Amersham Imager 600 Operating Instructions 29 0645 ...

Page 31: ...lor image of a chemiluminescence sample image and a white light image of a color marker Fluorescence imaging using blue green and red Epi light sources and UV trans illu minator Color images of colorimetrically stained gels or membranes obtained by white light illumination Accurate quantification of stained gels using white trans illumination Intuitive design with easy to use image analysis workfl...

Page 32: ... connected tablet computer It may also be operated from a wired touch screen or a monitor with mouse The illustration below displays Amersham Imager 600 setup with tablet computer 32 Amersham Imager 600 Operating Instructions 29 0645 17 AD 3 Instrument description 3 3 Illustration of the instrument ...

Page 33: ...ions Amersham Imager 600 RGB 29 0834 67 Amersham Imager 600 QC 29 0834 64 Amersham Imager 600 UV 29 0834 63 Amersham Imager 600 29 0834 61 Functions X X X X Control Software for image capture and analysis X X X X ChemiluminescenceCCD camera head including lens X X X X Epi White light source X X X Filter changer X X X Trans UV light source X X Trans White light source X Blue Green and Red Epi light...

Page 34: ... USB port 1 On Off button Press the button to turn on power Press again to turn off the power On Off button and LED indicators 2 Power The LED is lit white when the instru ment is powered on Run The LED is lit blue when the instrument is operating or if the Instrument indicator is selected Error The LED is lit red when the instrument is malfunctioning 34 Amersham Imager 600 Operating Instructions ...

Page 35: ...ad Air attachment iPad Air can be placed on the dock Instrument door Door 4 Toggle switch Power switch 5 I Power ON O Power OFF Connector for attaching the AC power cord Power connector 6 Port for connecting a digital only DVI monitor DVI D port 7 Port for additional external devices for exam ple a printer Note The tablet computer cannot be recharged with the instrument USB port 8 Connector for a ...

Page 36: ... position the tray Lower Tray guide 2 White light Trans White light source 3 UV light Trans UV light source 4 For configurations 600 600 UV and 600 QC White light source Epi light source 5 For configuration600 RGB White light plus Blue Green and Red light sources White light Epi light source 6 Filter changer unit Filter changer 7 36 Amersham Imager 600 Operating Instructions 29 0645 17 AD 3 Instru...

Page 37: ...er on Steady light X X Busy Run Flashing 1 Hz X X Error ON 0 5 s OFF 0 5 s Flashing 1 Hz X Shutdown ON 0 5 s OFF 0 5 s Flashing slowly 0 5 Hz X Power save ON 0 5 s OFF 1 5 s Mount iPad Air attachment The description below gives the necessary information for the mounting and dismounting of the iPad Air attachment to Amersham Imager 600 Mounting the attachment Place the attachment as illustrated bel...

Page 38: ...ide until you hear a clicking sound 2 Connect an iPad Air to the attachment 3 Dismounting the attachment Action Step Push the center of the attachment to release the lock points 1 38 Amersham Imager 600 Operating Instructions 29 0645 17 AD 3 Instrument description 3 4 Instrument hardware description ...

Page 39: ...Action Step Pull out the attachment downward 2 Amersham Imager 600 Operating Instructions 29 0645 17 AD 39 3 Instrument description 3 4 Instrument hardware description ...

Page 40: ...er a wire connected touch screen or a monitor plus mouse Note The available functions may vary depending on the system configuration Note For details on system configurations see Section 3 4 Instrument hardware descrip tion on page 33 In this section This section contains the following See page Section 41 3 5 1 Description of the Capture tab 46 3 5 2 Description of the Library tab 48 3 5 3 Descrip...

Page 41: ...describes the basic elements of the Capture tab 1 2 3 4 5 6 7 9 10 8 Function Part The selected image capturing method 1 Capture The tab for selecting image capturing method and starting ex posure 2 Area displaying options for the selected method 3 The settings icon for accessing the Settings view 4 The help icon for accessing context sensitive online help 5 Start The start exposure button 6 The d...

Page 42: ...re proceeding Method sensitive displays The display on the screen shows the selected image capturing method and presents the options relevant for that method Depending on system configuration you may or may not have access to all methods The following section describes all available method displays in the Amersham Imager 600 product family For details on system configurations see Section 3 4 Instr...

Page 43: ...olori metric marker 2 Exposure drop down menu use to select the exposure option for chemilu minescence image capturing Auto Semi Auto Manual Incremental and Avanced Note For more information on manual and incremental settings see Set exposure time on page 57 3 Start button use the button to start capturing 4 High dynamic range checkbox use the check box for image capture with high dynamic range 5 ...

Page 44: ...turing method 2 Epi illumination selected here Trans illumination Fluorescence The illustration below shows the specific components of the Capture tab with the Fluo rescence image capturing method Depending on selected options the display changes and unavailable options are hidden greyed out For more details of the general components see Capture view on page 41 44 Amersham Imager 600 Operating Ins...

Page 45: ... Auto selected here Semi Auto Manual Advanced Note For more information on manual settings see Set exposure time on page57 High dynamic range checkbox Use the check box for image capture with high dynamic range 4 Amersham Imager 600 Operating Instructions 29 0645 17 AD 45 3 Instrument description 3 5 Instrument software description 3 5 1 Description of the Capture tab ...

Page 46: ... selected file location here on the instrument use to view file storage options for example USB flash drive or network locations Select the drop down menu for more file location options 2 Search field use the field to search for specific files 3 Clear button use the button to clear the search field 4 Scroll buttons and bar use the bar or buttons to navigate among files in the displayed list of sto...

Page 47: ...se the buttons to select all files displayed or to deselect a selection of files 9 Delete button use the button to delete the selected marked file or files 10 Rename button use the button to rename a saved file 11 Copy Move button use the button to copy or move selected files 12 Open button use the button to open the selected file 13 Amersham Imager 600 Operating Instructions 29 0645 17 AD 47 3 In...

Page 48: ... 9 Function Part Display of the captured image Note Incremental and Advanced exposures may result in several images being displayed two at a time as they are exposed Navigate among the images using forward and backward arrows 1 48 Amersham Imager 600 Operating Instructions 29 0645 17 AD 3 Instrument description 3 5 Instrument software description 3 5 3 Description of the image view ...

Page 49: ... 93 If the colorimetric marker option was checked the tabs will display Sample and Marker If a multichannel fluores cence capture was per formed the tabs will dis play the selected channels of Blue Green and Red Information icon use to access additional image and exposure information such as image file name and exposure time 3 Amersham Imager 600 Operating Instructions 29 0645 17 AD 49 3 Instrumen...

Page 50: ...play is the default view 5 Done button Use the button to leave the view without saving Note A dialog appears asking to confirm the cancellation This prevents deleting images by accident 6 Save button use the button to save the image See Section 5 7 Save and manage image files on page 148 for further details 7 50 Amersham Imager 600 Operating Instructions 29 0645 17 AD 3 Instrument description 3 5 ...

Page 51: ...use the button to print the image Note The print function requires that the instrument is connected to a USB con nected printer For installation of a printer contact a representative from GE 9 Amersham Imager 600 Operating Instructions 29 0645 17 AD 51 3 Instrument description 3 5 Instrument software description 3 5 3 Description of the image view ...

Page 52: ...ze the image on page 160 Description The following illustration shows the start screen and first step of the analysis 1 2 3 4 5 6 Function Part Contrast button Use the button to change the contrast of the image 1 Image view button Use the button to activate the image view 2 Lane profile button Use the button to display separate graphical lane profiles 3 Zoom out button Use the button to zoom out 4...

Page 53: ...used in the subsequent analysis Open the background subtraction step with the button 2 Background is used to select method for background subtraction Open the band detection step with the button 3 Band Detection is used to detect the bands in each defined lane Amersham Imager 600 Operating Instructions 29 0645 17 AD 53 3 Instrument description 3 5 Instrument software description 3 5 4 Description ...

Page 54: ...e button 5 Normalization is used to select bands to be used for normalization Open the summary with the button 6 Summary is used to present an overview of the result data from the analysis Use the Save button to save the analysis data together with the image file 54 Amersham Imager 600 Operating Instructions 29 0645 17 AD 3 Instrument description 3 5 Instrument software description 3 5 4 Descripti...

Page 55: ...rn to the previous view 1 Network button Use to set up DHCP or Static IP network 2 Show mouse cursor check box Use to display a mouse cursor on a con nected external monitor by checking the check box 3 Instrument indicator button Use to activate the instrument connected to the tablet computer and light the instrument Run LED light 4 Date Time button Use to set date and time on the instrument 5 Foc...

Page 56: ...mponents of the keypad The image to the left shows the alphabetic view of the keypad with lower case letters The image to the right shows the numeric and symbol view of the keypad 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 1 2 4 5 7 8 9 10 Function Part The text field where the typed text is displayed 1 Clear button Use the button to remove all typed text in the text field 2 Keyboard area Use the keyboar...

Page 57: ...e Set exposure time view appears when you select Manual or Incremental exposure in the drop down menu and then select the Exposure time button or Interval time button It allows you to set the exposure time manually The illustration below shows the main components of the Set exposure time view 1 2 3 4 5 6 The time displayed in the Set exposure time window is by default the exposure time used during...

Page 58: ... Minutes Seconds and 1 10 sec fields The active field appears blue in the time units area 4 Use the numeric keys to set the time in the selected field Arrow buttons Use the buttons to navigate between minutes seconds and 1 10 s in the time units area 5 Set button Use the button to save the changes made 6 58 Amersham Imager 600 Operating Instructions 29 0645 17 AD 3 Instrument description 3 5 Instr...

Page 59: ...cription Part All configurations 29 0834 17 Black tray marked Black Tray 600 UV 29 0834 19 UV Trans tray marked UV Trans Tray 600 QC 600 RGB transparent 600 QC 29 0834 18 White Trans tray marked White Trans Tray 600 RGB All configurations 29 0880 60 White Insert marked White Insert for use with Black Tray where applicable Amersham Imager 600 Operating Instructions 29 0645 17 AD 59 3 Instrument des...

Page 60: ...ating Instructions All configurations 29 0902 97 DVDcontainingtransla tions of Amersham Imager 600 Operating Instructions All configurations 29 1540 84 Amersham Imager 600 Analysis Software All configurations 19 2448 01 AC power cord for North America All configurations 80 6480 33 AC power cord for Eu rope 60 Amersham Imager 600 Operating Instructions 29 0645 17 AD 3 Instrument description 3 6 Acc...

Page 61: ...9 iPad Air attachment Upgrade options Relevant for con figuration Code no Part Description 600 29 0834 22 AI600 Upgrade 600 to 600 UV 600 UV 29 0834 24 AI600 Upgrade 600 UV to 600 QC 600 QC 29 0834 25 AI600 Upgrade 600 QC to 600 RGB 600 UV 29 0834 26 AI600 Upgrade 600 UV to 600 RGB Amersham Imager 600 Operating Instructions 29 0645 17 AD 61 3 Instrument description 3 6 Accessories ...

Page 62: ...rt of the Amersham Imager 600 instrument must be per formed by GE authorized personnel The instrument contains parts that may be damaged unless they are secured for transportation and handled correctly Note In this chapter See page Section 63 4 1 Site requirements 65 4 2 Instrument setup 66 4 3 System settings 74 4 4 Network 62 Amersham Imager 600 Operating Instructions 29 0645 17 AD 4 Installatio...

Page 63: ...ch must be level within 2º two degrees 1 Take into considerationthe workflow and ancillary facilities when planning the installation 2 Any required construction and electricity air conditioning work must be completed prior to installation 3 It is not desirable to have a heat source near the Amersham Imager 600 air intakes even if the other environmental require ments have been met 4 Do not install...

Page 64: ...perature can vary widely with temperature fluc tuation above 10ºC per hour 2 near a heat source such as a radiator 3 where it may get wet or flooded 4 where it may be exposed to corrosive gas 5 in a dusty environment 6 in a place constantly or excessively exposed to vibration or im pacts 7 in a place exposed to direct sunlight Indoor use Operation site 2000 m or lower Maximum operating altitude 70...

Page 65: ...Imager 600 must be secured before long distance transports Contact GE for instructions before transport or relocation of the instrument For connecting additional monitors or peripheral equipment contact GE for instructions Amersham Imager 600 Operating Instructions 29 0645 17 AD 65 4 Installation 4 2 Instrument setup ...

Page 66: ...tings view Open the settings view by selecting the settings icon found in the top right hand corner of the Capture and Library tabs For the general components of the Settings view see Section 3 5 5 Description of settings views on page 55 66 Amersham Imager 600 Operating Instructions 29 0645 17 AD 4 Installation 4 3 System settings ...

Page 67: ...h and day 2 Time field Use the plus and minus buttons next to Hour and Minute to set values for hour and minute 3 Ok button Use the button to save the changes to date and time and return to the Settings window 4 Change date To change the date setting on the instrument select the plus or minus buttons next to the Year Month and or Day field to change the value of year month or day Amersham Imager 6...

Page 68: ...ield to change the value of hour or minute Save the changes to date and time Select the Ok button to save the changes to the Date time view Select the Cancel button to leave the view without saving changes 68 Amersham Imager 600 Operating Instructions 29 0645 17 AD 4 Installation 4 3 System settings 4 3 1 Date and time ...

Page 69: ...value 3 Single arrow buttons Use the buttons to adjust focus in smaller steps 4 Default button Use the button to reset focus to the default value Note The default value differs between instruments 5 The brightness value 6 Plus and minus buttons Use the buttons to adjust the brightness value 7 OK button Use the button to save the new settings 8 Cancel button Use the button to leave the view without...

Page 70: ... the arrows above and below the Focus value 4 Select OK to save the settings Note Cancel will close the view without saving changes 5 The new focus will be available for captures until a new capturing method is selected or until the instrument enters standby mode 6 Prior to the capture confirm the use of the new focus with Yes Selecting the No button cancels the adjustments to focus and the normal...

Page 71: ... same location It identifies which instrument is connected to a certain tablet computer Select the Instrument indicator button This activates the Run LED light on the instrument it is connected to Amersham Imager 600 Operating Instructions 29 0645 17 AD 71 4 Installation 4 3 System settings 4 3 3 Instrument indicator ...

Page 72: ... Follow the instructions below to update the software for Amersham Imager 600 Action Step Connect a USB flash drive containing the update file provided by GE stored at the root level of the USB flash drive 1 Select the Software update button 2 Confirm USB insertion by selecting the OK button 3 Select the Yes button to update the software 4 72 Amersham Imager 600 Operating Instructions 29 0645 17 A...

Page 73: ...tion Step Select the Export log button 1 Result A text file will be created on the instrument containing the export log file Insert a USB flash drive and confirm insertion by selecting the OK button 2 Result The export log file will be transferred to the USB flash drive To return to the Settings view select the OK button 3 Amersham Imager 600 Operating Instructions 29 0645 17 AD 73 4 Installation ...

Page 74: ...and delete image files from a computer This section contains information on network setup options virus protection network settings and file access In this section See page Section 75 4 4 1 Network setup 79 4 4 2 Network access from instrument 81 4 4 3 Instrument access from network 74 Amersham Imager 600 Operating Instructions 29 0645 17 AD 4 Installation 4 4 Network ...

Page 75: ...t connect telephone lines to the Ethernet port Only the IEC 60950 I VL 60950 1 standard non shielded cables are appropriate for connection to this connector Connection to a network enables the instrument to access files from and to save files to a network location There are two options for network setup 1 DHCP 2 Static IP If a DHCP network is used the IP address will be assigned automatically by t...

Page 76: ...ppear Select the Network button 2 Result The Network view will appear Select the type of network connection and follow the separate instructions below 3 Connect to a DHCP network Follow the instructions below to connect to a DHCP network 76 Amersham Imager 600 Operating Instructions 29 0645 17 AD 4 Installation 4 4 Network 4 4 1 Network setup ...

Page 77: ...ge 56 3 Select OK 4 Result An automatic connection to the DHCP network is established within a couple of seconds Tip Reserve the IP address to Amersham Imager 600 for easier access of its net work resources Connect to a Static IP network Follow the instructions below to connect to a Static IP network Action Step Connect the network cable to the Ethernet port on Amersham Imager 600 1 Amersham Image...

Page 78: ...d to enter the subnet mask If desired select the Gateway field and use the keypad to enter the router Workgroup field and use the keypad to enter the workgroup Select OK to save the changes 4 Result The connection to the network will be established within a couple of seconds 78 Amersham Imager 600 Operating Instructions 29 0645 17 AD 4 Installation 4 4 Network 4 4 1 Network setup ...

Page 79: ...the network identification name 1 Create a new folder and name the folder 2 Example AmershamImager600 Right click on the folder and select Properties Share with 3 Select which user or group you wish to grant permission for example users and check the check box Full control Note Limited permissions such as Read will not allow the instrument to export or analyze saved files on the computer 4 Select ...

Page 80: ...sharing is turned on you must have a user account and password on your computer for full access to shared items Password protected sharing is located in the Control panel under Advanced sharing settings Pass word protection is turned on by default Note 80 Amersham Imager 600 Operating Instructions 29 0645 17 AD 4 Installation 4 4 Network 4 4 2 Network access from instrument ...

Page 81: ...e Network view Open the view by selecting the settings icon in the top right hand corner and then select the Network button in the Settings view DHCP networks has a dynamic IP address assignment therefore the IP address may vary between connections When using a DHCP network connection check regularly that the IP address is still accurate If the IP address has changed use the new IP address in the ...

Page 82: ...p 192 168 0 25 8080 Result A page asking for password is displayed Note The password can be changed Press Option in the Login window and follow the instruction in the Options window 82 Amersham Imager 600 Operating Instructions 29 0645 17 AD 4 Installation 4 4 Network 4 4 3 Instrument access from network ...

Page 83: ...to be copied or deleted by checking the file s check box Note A maximum number of 5 files can be copied at one time You may repeat this step to copy additional files 5 Select the Copy button to copy the selected files Select the Delete button to delete the selected files Note The copied files will be downloaded as a zip file with an automatically gener ated name The download location and procedure...

Page 84: ...Safety precautions on page 12 for details WARNING Do not operate Amersham Imager 600 in any other way than de scribed in the User Manual In this chapter See page Section 85 5 1 Operation flow chart 86 5 2 Turn on the instrument 89 5 3 Select tray 90 5 4 Place the tray in the instrument 93 5 5 Capture an image 144 5 6 View the image 148 5 7 Save and manage image files 160 5 8 Analyze the image 177 ...

Page 85: ...n is as follows Select a tray and place sample Place the tray in the instrument Capture the image Analyze the image Save the image file Turn on the instrument Amersham Imager 600 Operating Instructions 29 0645 17 AD 85 5 Operation 5 1 Operation flow chart ...

Page 86: ...ont of the instrument to activate it 2 Result The instrument s self diagnostic process will initialize Note The self diagnostic process will normally take approximately 5 minutes Connection to a network may take longer If relevant connect a USB flash drive to the instrument for file storage 3 The USB flash driver is automatically installed when the USB flash drive is connected to Amersham Imager 6...

Page 87: ... Imager 600 on the desktop screen of the connected tablet computer 4 Result 1 A welcome screen appears 2 The following screen will be displayed Amersham Imager 600 Operating Instructions 29 0645 17 AD 87 5 Operation 5 2 Turn on the instrument ...

Page 88: ...nue button 5 Result The following screen will be displayed Wait for the CCD status to turn green 6 Result Image capturing can begin 88 Amersham Imager 600 Operating Instructions 29 0645 17 AD 5 Operation 5 2 Turn on the instrument ...

Page 89: ...QC 600 RGB 600 Black Tray Membrane Gel Colorimetric Epi illumination 600 UV 600 QC White Insert or White Trans Tray 600 RGB 600 QC White Trans Tray Gel Colorimetric Trans illumination 600 RGB Diffuser Board 600 RGB Black Tray Gel Membrane Fluorescence RGB Epi illumination 600 UC UV Trans Tray transparent Gel Fluorescence Trans UV 600 QC Gel sheet 600 RGB NOTICE With regards to third party patents ...

Page 90: ...ct tray position in Amersham Imager 600 Action Step Select a tray and accessories suitable for the capturing method 1 For Chemiluminescence with colorimetric marker and Colorimetric Epi capturing place the White Insert inside the Black Tray 2 90 Amersham Imager 600 Operating Instructions 29 0645 17 AD 5 Operation 5 4 Place the tray in the instrument ...

Page 91: ...h different fields of view 1 One side indicates the maximum field of view of 8 x 11 cm for the upper tray position Use the upper tray position for Chemiluminescence captur ing for a smaller field of view of a single membrane and shorter exposure time 2 The other side indicates the maximum field of view of 16 x 22 cm for the lower tray position Use the lower tray position for a larger field of view...

Page 92: ...portant to avoid capturing artefacts in the image Open the instrument door 4 Insert the tray in the correct tray position Note For Chemiluminescence capturing you may also select the upper tray position 1 in the illustration below 5 1 2 Close the door 6 92 Amersham Imager 600 Operating Instructions 29 0645 17 AD 5 Operation 5 4 Place the tray in the instrument ...

Page 93: ...y of the available methods In this section See page Section 94 5 5 1 Available methods 95 5 5 2 Chemiluminescence capture 118 5 5 3 Colorimetric capture 122 5 5 4 Fluorescence capture Amersham Imager 600 Operating Instructions 29 0645 17 AD 93 5 Operation 5 5 Capture an image ...

Page 94: ...n x x Colorimetric Trans illumination x x x Fluorescence UV trans illumination x Fluorescence Blue Green Red Epi illumination single channel or multichannnel x Fluorescence Blue Green Red Epi illumination with colorimetric marker single channel NOTICE Do not open the instrument door during exposure when the RUN light indicates operation This will stop the exposure and data will not be saved 94 Ame...

Page 95: ...apturewithcolorimet ric markers is performed on one to four mem branes placed on a Black Tray with a White In sert Note The White Insert has 2 sides one for upper tray position and the other for the lower tray position 1 2 There are two alternative tray positions 1 Upper tray position Use the upper tray position for a smaller field of view of a single membrane and shorter exposure time The field o...

Page 96: ...he sample To use the full dynamic range of the image file check High dynamic range To minimize exposure time leave High dynamic range unchecked Select Semi automatic A specific area of a sample is of interest A short pre exposure is performed which results in an image This allows you to select the area of the sample image to focus on The software automatically calculates the optimal exposure time ...

Page 97: ...ations Do not open the door during exposure as this will stop the exposure and the data will not be saved Note Chemiluminescence with automatic exposure time Follow the instructions below to perform chemiluminescence capture using automatic exposure time Action Step In the Capture tab select Chemiluminescence 1 Result The options available for Chemiluminescence capturing are displayed to the right...

Page 98: ... unchecked Select Start 4 Result The exposure will begin following a pre exposure A progress bar appears on the screen indicating the time remaining of the imaging process the exposure selected and the calculated remaining time Note Selecting Cancel will cancel the exposure and the Capture view will appear 98 Amersham Imager 600 Operating Instructions 29 0645 17 AD 5 Operation 5 5 Capture an image...

Page 99: ...mages are displayed under Sample and Marker tabs Colorimetric marker was used Select the relevant tab for the image you wish to display A selected tab is highlighted with blue text and frame Select both tabs simultaneously to see a composite color image of the Sample and Marker images Select the Save button to save the images 6 For other options after capturing see Options after capturing on page ...

Page 100: ...e right on the screen If your sample contains a colorimetric marker then select the check box Colorimetric marker 2 To use the full dynamic range of the image file check High dynamic range 3 To minimize exposure time leave High dynamic range unchecked Select Semi Auto exposure in the exposure drop down menu 4 Select Next and wait for the pre exposure to finish 5 100 Amersham Imager 600 Operating I...

Page 101: ... to get an optimal view of the area of interest Choose blue arrows to adjust the lower range of the grayscale in the displayed image indicated by the blue line in the image histogram Choose red arrows to adjust the upper range of the grayscale in the displayed image indicated by the red line in the image histogram Amersham Imager 600 Operating Instructions 29 0645 17 AD 101 5 Operation 5 5 Capture...

Page 102: ...ed with an X Choose and then select two diagonal corners of an area of interest in the image Result The selected area is highlighted with a square Use the right hand side arrow buttons to move the highlighted point or area Select Start exposure and wait for the exposure to finish 8 102 Amersham Imager 600 Operating Instructions 29 0645 17 AD 5 Operation 5 5 Capture an image 5 5 2 Chemiluminescence...

Page 103: ... are displayed under Sample and Marker tabs Colorimetric marker was used Select the relevant button for the image you wish to display A selected tab is highlighted with blue text and frame Select both buttons simultaneously to see a com posite color image of the Sample and Marker im ages Select the Save button to save the images 10 For other options after capturing see Options after capturing on p...

Page 104: ... contains a colorimetric marker then check the check box Colorimetric marker 2 Select Manual exposure in the exposure drop down list 3 To change the exposure time select the exposure time field 4 Result The Set exposure time view opens Enter the new time and select Set to return to the previous view For detailed instructions see Section 3 5 5 Description of settings views on page 55 5 104 Amersham...

Page 105: ...as used The resulting images are displayed under Sample and Marker tabs Colorimetric marker was used Select the relevant tab for the image you wish to display A se lected tab is highlighted with blue text and frame Select both tabs simultaneously to see a composite color image of the Sample and Marker images Select the Save button to save the images 8 For other options after capturing see Options ...

Page 106: ...ct Incremental exposure in the exposure drop down menu 2 Set the number of repetitive exposures in the Expose field using the plus and minus buttons 3 Check the check box Stop when saturated if you wish the exposure to stop when the pixel intensity of the accumulated exposed images reaches satu ration 4 Note The Stop when saturated function overrides the set repetitive exposures 106 Amersham Image...

Page 107: ...epeatedly for the set time period or until saturate During repeated image capture the contrast of the images can be adjusted by pressing Auto This adjustment can be reset with the Reset button 7 Result The number of images matchingthe selected repetitionsare displayed as they are exposed A progress bar indicates the time remaining of the imaging process Amersham Imager 600 Operating Instructions 2...

Page 108: ...which image or images you wish to save Then select the Save button Note If you select more than one image then the Library view will open after se lecting Save 9 NOTICE When using incremental exposure images that are not selected will be deleted at saving Chemiluminescence with advanced exposure Follow the instructions below to perform chemiluminescence capture with Advanced exposure mode This exp...

Page 109: ...or chemiluminescence capturing are displayed to the right on the screen If your sample contains a colorimetric marker then check the Colorimetric marker check box 2 Amersham Imager 600 Operating Instructions 29 0645 17 AD 109 5 Operation 5 5 Capture an image 5 5 2 Chemiluminescence capture ...

Page 110: ... specified depending on the condi tions The table below shows the available combinations No colorimetric marker Colorimetric marker Lower Lower Tray position 3 levels 3 levels Sensitivity 4 areas 1 area Image area 1 12 1 No of captures 110 Amersham Imager 600 Operating Instructions 29 0645 17 AD 5 Operation 5 5 Capture an image 5 5 2 Chemiluminescence capture ...

Page 111: ...w summarizes the availability of sensitivity levels for different tray positions Sensitivity level Tray position High binning x4 Standard Upper position Highest binning x16 High binning x4 Standard Lower position Note This function increases sensitivity by binning pixels and results in lower image resolution Amersham Imager 600 Operating Instructions 29 0645 17 AD 111 5 Operation 5 5 Capture an im...

Page 112: ...tray position with standard sensitivity and without colorimetric maker 5 Set the number of repetitive exposures in Number of captures by using the plus and minus buttons 6 112 Amersham Imager 600 Operating Instructions 29 0645 17 AD 5 Operation 5 5 Capture an image 5 5 2 Chemiluminescence capture ...

Page 113: ...iew The Reset button resets the value to zero Set the exposure time by pressing value 8 Result the Set exposure time view opens Enter the value and select Set to save the value and to return to the previous view The Reset button resets the value to zero Amersham Imager 600 Operating Instructions 29 0645 17 AD 113 5 Operation 5 5 Capture an image 5 5 2 Chemiluminescence capture ...

Page 114: ...Action Step Select Start exposure 9 114 Amersham Imager 600 Operating Instructions 29 0645 17 AD 5 Operation 5 5 Capture an image 5 5 2 Chemiluminescence capture ...

Page 115: ...hlighted with blue text and frame Select both buttons simultaneously to see a composite color image of the Sample and Marker images When the exposure is finished the image view appears dis playing the image Amersham Imager 600 creates a folder for the image files For details see File formats on page 154 Select the Save to save the images Images are captured several times with the set waiting and e...

Page 116: ...mager 600 creates a folder for the image files For details see File formats on page 154 Use the Select to select the images to be saved then Save to save the images Note If more than one image is selected then the Library view will open after selecting Save NOTICE When using advanced exposure images that are not selected will be deleted at saving 116 Amersham Imager 600 Operating Instructions 29 0...

Page 117: ...s To modify the display settings or view details of the image select the image tools to the right of the image See Image tools on page 145 for further instructions To analyze the image select Analyze See Section 5 8 Analyze the image on page 160 for further instructions To print the image select Print To discard the image without saving select Done and confirm the following dialog Amersham Imager ...

Page 118: ...ource at the bottom of the instrument interior When using Trans illumination the intensity data is converted to optical density OD values which provides a more quantitative result compared to Epi illumination Colorimetric capture is only available with Lower tray position Note The images are by default created with automatic exposure time The table below describes details on accessories and tray p...

Page 119: ...ow the instructions in the table to perform colorimetric capture Action Step In the Capture tab select Colorimetric 1 Result The options available for colorimetric capturing are displayed to the right on the screen Amersham Imager 600 Operating Instructions 29 0645 17 AD 119 5 Operation 5 5 Capture an image 5 5 3 Colorimetric capture ...

Page 120: ...his is important to avoid artefacts in the image 2 Note 1 For Trans illumination place a diffuser board at the bottom of the instrument with the matte side up before capturing Select Start 3 Result A progress bar appears on the screen indicating the time remaining of the imaging process 120 Amersham Imager 600 Operating Instructions 29 0645 17 AD 5 Operation 5 5 Capture an image 5 5 3 Colorimetric...

Page 121: ...fter capturing To save the image select Save This will allow you to reopen the image later to use the image tools to perform an analysis or to print the image See Section 5 7 Save and manage image files on page 148 for further instructions To modify the display settings or view details of the image select the image tools to the right of the image See Image tools on page 145 for further instruction...

Page 122: ... x x x Trans UV The Custom method allows for different combinations of the blue green or red light sources and an emission filter of choice Note Fluorescence capture options The table below illustrates sample types for fluorescence capturing Light source location in side Tray and accessory placement only in the lower tray position Sample type Epi Blue Green and Red fluorescence top Black Tray Gel ...

Page 123: ...ynamic range unchecked Select Semi automatic A specific area of a sample is of interest A short pre exposure is performed which results in an image This allows you to select the area of the sample image to focus on The software automatically calculates the exposure time for the specified area To use the full dynamic range of the image file check High dynamic range To minimize exposure time leave H...

Page 124: ...p In the Capture tab select Fluorescence 1 Result The options available for fluorescence capturing are displayed to the right on the screen To use the full dynamic range of the image file check High dynamic range 2 To minimize exposure time leave High dynamic range unchecked Note This function is only effective with the Auto and Semi Auto exposure settings 124 Amersham Imager 600 Operating Instruc...

Page 125: ... to the next step For Advanced exposure see Fluorescence RGB with Advanced exposure on page 132 If exposure option Manual was selected then select the Exposure time Set button 5 Note If more than one detection channel was selected the Exposure time button displays Set If only one detection channel was selected the button displays the latest set exposure time Result This opens the Set exposure time...

Page 126: ... Set Result This will close the view For details see Set exposure time on page 57 Select Start A progress bar appears on the screen indicating the time re maining of the imaging process and the selected exposure option 7 126 Amersham Imager 600 Operating Instructions 29 0645 17 AD 5 Operation 5 5 Capture an image 5 5 4 Fluorescence capture ...

Page 127: ... is highlighted with blue text and frame Select two or more tabs simultaneously to see a composite color image Twodetectionchan nels were selected Select the relevant tab of Blue Green or Red for the image you wish to display A selected tab is highlighted with blue text and frame Select both tabs simultaneously to see a composite color image Onedetectionchan nel was selected Sample One image is di...

Page 128: ...ure with a colorimetric marker Action Step In the Capture tab select Fluorescence 1 Result The options available for fluorescence capturing are displayed to the right on the screen Check the Epi RGB radio button and then check the relevant check box of Blue Green or Red 2 Check the Colorimetric marker check box 3 128 Amersham Imager 600 Operating Instructions 29 0645 17 AD 5 Operation 5 5 Capture ...

Page 129: ...ure on page 132 If Auto or Manual exposure was chosen select Start 5 Result A progress bar appears on the screen indicating the time remaining of the imaging process and the selected exposure option Proceed to the final step of this instruction If Semi Auto exposure was chosen select Next and wait for the pre exposure to finish 6 Result A pre exposed image appears Amersham Imager 600 Operating Ins...

Page 130: ...rea of interest Choose blue arrows to adjust the lower range of the grayscale in the displayed image indicated by the blue line in the image histogram Choose red arrows to adjust the upper range of the grayscale in the displayed image indicated by the red line in the image histogram Note The colorimetric marker is not displayed in the preview 130 Amersham Imager 600 Operating Instructions 29 0645 ...

Page 131: ... finish 9 When the exposure is finished the image view appears displaying the images Amersham Imager 600 creates a folder containing the created image files which can be saved 10 For details see File formats on page 154 Select the relevant tab of Sample and Marker for the image you wish to display A selected tab is highlighted with blue text and frame Select both tabs simultaneously to see a compo...

Page 132: ...er tray position Note Action Step In the Capture tab select Fluorescence 1 Result The options available for fluorescence capturing are displayed to the right on the screen Note Advanced exposure mode is available with only one channel of Epi RGB or a custom channel under Fluorescence RGB capture If the sample contains a colorimetric marker then check the Colorimetric marker box 2 132 Amersham Imag...

Page 133: ...n be specified depending on the condi tions The table below shows the available combinations No Colorimetric marker Colorimetric marker Lower Lower Tray position 3 levels 3 levels Sensitivity 4 areas 1 area Image area 1 12 1 Noumber of captures Amersham Imager 600 Operating Instructions 29 0645 17 AD 133 5 Operation 5 5 Capture an image 5 5 4 Fluorescence capture ...

Page 134: ...y level Tray position Highest binning x16 High binning x4 Standard Lower position Note This function increases sensitivity by binning pixels and results in lower image resolution Select an area in Image area This is only available for image capture in the lower tray position with standard sensitivity and without colorimetric maker 5 134 Amersham Imager 600 Operating Instructions 29 0645 17 AD 5 Op...

Page 135: ...aiting time by pressing value 7 Result this opens the Set waiting time view Enter the value and select Set to save the value and to return to the previous view The Reset button resets the value to zero Amersham Imager 600 Operating Instructions 29 0645 17 AD 135 5 Operation 5 5 Capture an image 5 5 4 Fluorescence capture ...

Page 136: ...exposure time view opens Enter the value and select Set to save the value and to return to the previous view The Reset button resets the value to zero 136 Amersham Imager 600 Operating Instructions 29 0645 17 AD 5 Operation 5 5 Capture an image 5 5 4 Fluorescence capture ...

Page 137: ...Action Step Select Start exposure 9 Amersham Imager 600 Operating Instructions 29 0645 17 AD 137 5 Operation 5 5 Capture an image 5 5 4 Fluorescence capture ...

Page 138: ...utton is highlighted with blue text and frame Select both buttons simultaneously to see a composite color image of the Sample and Marker images When the exposure is finished the image view appears dis playing the image Amersham Imager 600 creates a folder for the image files For details see File formats on page 154 Select the Save to save the images Images are captured several times with the set w...

Page 139: ...er for the image files For details see File formats on page 154 Use the Select to select the images to be saved then Save to save the images Note If more than one image is selected then the Library view will open after selecting Save NOTICE Whenusingadvancedexposure mode imagesthatare not selected will be deleted at saving Amersham Imager 600 Operating Instructions 29 0645 17 AD 139 5 Operation 5 ...

Page 140: ...escence Trans UV sample preparation Follow the instructions below to prepare the sample Action Step Cut out a gel sheet slightly larger than the sample size 1 Place the gel sheet on the UV Trans Tray transparent tray 2 Place the sample on the gel sheet 3 Proceed with the capturing Note After use wash the gel sheet with mild detergent rinse with water then dry well A gel sheet can be reused about 2...

Page 141: ...ize exposure time leave High dynamic range unchecked Note This function is only effective with Auto and Semi Auto exposure setting If Auto or Manual exposure was selected select Start 5 Result A progress bar appears on the screen indicating the time remaining of the imaging process and the exposure time Proceed to the final step of this instruction If Semi Auto exposure was selected select Next 6 ...

Page 142: ...ws to get an optimal view of the area of interest Choose blue arrows to adjust the lower range of the grayscale in the displayed image indicated by the blue line in the image histogram Choose red arrows to adjust the upper range of the grayscale in the displayed image indicated by the red line in the image histogram 142 Amersham Imager 600 Operating Instructions 29 0645 17 AD 5 Operation 5 5 Captu...

Page 143: ...and side arrow buttons to move the highlighted point or area Press Start exposure and wait for the exposure to finish 9 When the exposure is finished the image view displays the image Amersham Imager 600 creates a folder containing the image files which can be saved 10 For details see File formats on page 154 Select the Save button to save the images 11 For other options after capturing see Option...

Page 144: ...f the image view on page 48 1 2 3 4 Function Part The captured image is displayed 1 Tabs for captured images Use the tab to select or deselect images of the different detection channels Note A displayed channel is indicated with a tab in blue text and blue frame All three channels can be selected simultaneously A non displayed channel is indicated with a black text and gray frame 2 Image tools men...

Page 145: ...und and white bands Use the Rotate 90 degrees left or right buttons to rotate the image Rotation changes will be saved in the image file Use the Edit image buttons to edit the image into separate image files The selected edit option is marked and red lines illustrate the edit in the image Edits will be saved in the image file Open the Zoom tool by selecting the zoom button in the image tools menu ...

Page 146: ...he number of pixels for each intensity value in the captured image The horizontal axis represents inten sity variations The vertical axis represents the number of pixels with a given intensity The blue and red lines indicate the min max range of the grayscale in the displayed image Use the blue and red arrows to modify the contrast Note For samples with colorimetric marker use the contrast tool fo...

Page 147: ...able tool Navigate between the images using the forward and backward arrows Point to a specific area of interest to dis play the maximum pixel intensisty and the average pixel intensity in the area Tip If the intensity is too low or too high consider to capture the image using a different exposure time Too high intensity results in saturation and prevents quan titative analysis Saturation occurs c...

Page 148: ...an access and manage files stored on the network location This section describes the locations where you can save files how to save a file the various file formats and the file management options NOTICE Transferring large amounts of files to a connected computer during operation can impair the system s performance 148 Amersham Imager 600 Operating Instructions 29 0645 17 AD 5 Operation 5 7 Save an...

Page 149: ...ible providing the instrument has been granted access to the network location For more details see Section 4 4 Network on page74 Note Connect a USB flash drive A USB flash driver is automatically installed when the USB flash drive is connected to Amersham Imager 600 Password protected USB flash drives are not supported Note Add new save location Follow the instructions to add a location for file s...

Page 150: ...folder can be added as a location for saving files For information on how to configure a network see Section 4 4 Network on page 74 5 In the Choose save location view mark the network folder you wish to add and select the Use this location button 6 Ensure that the path to this location appears in the Location field 7 Select the OK button to save the added location The Cancel button will leave the ...

Page 151: ...in the top right hand corner of the screen 1 Select the Save locations button 2 Select the Edit button 3 If desired edit the name for the save location in the Name field 4 If desired edit the folder for the save location by selecting the Browse button next to the Location field Note The network settings must be properly configured before a network folder can be added as a location for saving files...

Page 152: ... Result The Save view opens The automatically generated image name s are displayed in the lower part of the view Note If an edited split image is saved then the screen will display the edited split suffix Example Note 152 Amersham Imager 600 Operating Instructions 29 0645 17 AD 5 Operation 5 7 Save and manage image files 5 7 2 Save images ...

Page 153: ...lect the Comment field This opens a keypad view Type you comment and then select Done to return to the previous view 4 Select Save to save the file s The saved files can be accessed via the Library tab See Section 3 5 2 Description of the Library tab on page 46 for more details 5 If capturing is finished for the day switch off the instrument by pressing the On Off button on the front panel Wait un...

Page 154: ...he following files Chemiluminescence Sample A tif image in gray scale for quantificationof chemiluminescencedata Marker A tif image in gray scale for quantification of colorimetric data Note Both the above files have the same pixel resolution Sample Marker A jpg composite im age of chemiluminescenceand colorimet ric data in color and with set contrast Note The composite image is displayed if both ...

Page 155: ...ning the following files Fluorescence Sample A tif in gray scale for quantifi cation of fluorescence data Marker A tif in gray scale for quan tification of colorimetric data Note Both the above files have the same pixel resolution Sample Marker A jpg composite im age of fluorescence and colorimetric data in color and with set contrast Note The composite image is displayed if both tabs are selected...

Page 156: ...for quantifica tion of fluorescence data Note The number of tif files created correspond to the number of selected detection chan nels Blue Green Red A jpg composite image of fluorescence data in color and with set contrast Note The composite image is displayed if two or three tabs are selected simultaneously Multichannel imaging 156 Amersham Imager 600 Operating Instructions 29 0645 17 AD 5 Opera...

Page 157: ...he files in the library Delete files Follow the instruction to delete files from the device Action Step Select the file s to delete 1 Select the Delete button 2 Result The following window will appear Amersham Imager 600 Operating Instructions 29 0645 17 AD 157 5 Operation 5 7 Save and manage image files 5 7 3 File manage options ...

Page 158: ...appear Select the location to copy or move the file s to in the drop down menu in the Move or copy selected files to field 3 Select the Copy button to copy the file s to the desired location 4 Select the Move button to move the file s to the desired location Note When a file is copied the file will remain in the original location thus be stored in two places 158 Amersham Imager 600 Operating Instr...

Page 159: ...eypad view on page 56 Open files Follow the instruction to open files Action Step Select the file to open 1 Select the Open button to open the file 2 If desired use the Image tools to adjust the view of the image 3 For detailed information on the Image tools see Image tools on page 145 When finished select the Done button to go back to the Library tab 4 Amersham Imager 600 Operating Instructions 2...

Page 160: ...alysis results The workflow is performed in five steps and the final result is presented in a summary You can analyze a newly captured image or analyze a previously saved image Select the Analyze button in the image view to open the analysis workflow view The following illustration and table describe the start view of the analysis workflow 160 Amersham Imager 600 Operating Instructions 29 0645 17 ...

Page 161: ...ion Function Part The current step in the analysis workflow highlighted 1 Back button Use the button to close the analysis workflow 2 Amersham Imager 600 Operating Instructions 29 0645 17 AD 161 5 Operation 5 8 Analyze the image ...

Page 162: ...Zoom buttons Use the button to zoom in on or zoom out of the image Navigation arrow buttons Use the button to move the image on the screen The automatically created lane grid and lane numbers 4 The displayed image tab highlighted with a blue frame 5 Options and steps associated to the current step in the analysis 6 Help icon Use to reveal additional information on the selected step in the analysis...

Page 163: ...lane grid to correspond with the lanes in the image Adjust the lane grid Follow the instructions below to adjust the lane grid to match lanes in the image Action Step Select the minus and plus buttons adjacent to Number of lanes to increase or decrease the number of grid lanes to correspond with the number of lanes in the image 1 Select a grid corner circle 2 Result The selected corner circle is h...

Page 164: ...ns adjacent to Lane width to increase or decrease the lane fill factor Note If the value is 100 then the grid contains only the lanes Using a lesser per centage creates an empty space between lanes 4 When you are done select the Next button or select the Background button in the workflow The Back button will bring you back to the captured image 5 164 Amersham Imager 600 Operating Instructions 29 0...

Page 165: ...racts background for all lanes in the image Note Use the Show background check box to see how each background subtraction options works This function shows the background level calculated by each option Tip Description Option Use this option when no background should be subtracted from the image None Use this option to subtract the background below a base line between the lowest points on the lane...

Page 166: ... will subtract more background than a large Rolling ball Subtract background Action Step If an image of the sample is displayed select the lane profile button 1 Result A screen will appear displaying the lane profile graphically with the current lane number displayed in the drop down list and the lane profile button in blue 166 Amersham Imager 600 Operating Instructions 29 0645 17 AD 5 Operation 5...

Page 167: ...d radio buttons 3 None Rubber band Minimum profile Rolling ball and then select the Radius plus or minus buttons to set the radius for the virtual rolling ball eraser to set the new baseline of the graph Tip The smaller the radius the more background will be removed Amersham Imager 600 Operating Instructions 29 0645 17 AD 167 5 Operation 5 8 Analyze the image 5 8 2 Background Subtraction ...

Page 168: ... result in any lane select the lane number to view in the drop down menu When you are done select the Next button or select the Bands button in the workflow The Previous button will bring you back to the previous step 5 168 Amersham Imager 600 Operating Instructions 29 0645 17 AD 5 Operation 5 8 Analyze the image 5 8 2 Background Subtraction ...

Page 169: ...e green channel is displayed with a green frame etc Detecting bands Follow the instructions below to detect bands Action Step Select the Detect button to detect the bands automatically 1 Tip Shift between lane profile image view and image tabs to verify the result of the band detection If the band detection is satisfactory then proceed to the final step in this table If too many bands are detected...

Page 170: ... arrow buttons To adjust a band width select the band and adjust it with the Width plus and minus buttons To delete a band select the band and press Delete To delete all detected bands in the image select Clear Repeat actions in step 3 for other detection channels if relevant 4 When you are done detecting bands press the Next button or select the MW button in the workflow The Previous button will ...

Page 171: ...lecular weight calibration can only be performed in single channel view Note Action Step Select the lane containing the molecular weight marker 1 Result The selected lane appears highlighted Select the standard molecular weight marker from the drop down menu 2 If the correct marker is missing Add a missing marker by selecting Add new button See instructions below Edit a marker by selecting the Edi...

Page 172: ...ed molecular weight is presented in the summarized table in Summary Note Addamolecularweightstandard marker Action Step Select the Add new button 1 Result The following view appears Select the Name field and type the name of the marker 2 Select the unit from the Unit drop down menu 3 Type a value and select the Add button 4 Result The value will appear in the bottom of the list to the left on the ...

Page 173: ...ear in the list Note You may need to scroll to see the new value Select the Save button to save the edited values and to return to the MW Calibration view Select Cancel to leave without saving changes Delete will delete the entire marker group 4 To delete a value select the X next to the value Note Amersham Imager 600 Operating Instructions 29 0645 17 AD 173 5 Operation 5 8 Analyze the image 5 8 4...

Page 174: ...e selected band volumes Normalization is useful when comparing relative band volumes in different lanes The options for normalization are presented next to the image Normalize bands Follow the instructions below to perform normalization Action Step Select one or two control bands in a lane 1 Result The selected band s appear highlighted Note Control bands can only be selected in single channel vie...

Page 175: ... printer 3 Columns displaying 4 Ch the detection channel Lane the number of the lane change the sort order by selecting up or down arrow in the column heading Band the number of the band from top to bottom Volume the integrated signal intensity in the specific band Lane the relative band volume within a lane in percentage Normalized value the band volume relative to the control band volume Mw the ...

Page 176: ...name by selecting the Image name prefix field Type the new prefix using the appearing keypad and select the Done button to save the changes 5 If desired add a comment by selecting the Comment field Type the com ment using the appearing keypad and select the Done button to save the changes 6 Press Save 7 In addition to the image file s analyzed data is saved with a CSV file and the summary view is ...

Page 177: ...of the instrument to the O position 2 Result The instrument is turned off To optimize the instrument s life time and to minimize unnecessary power con sumption shut down the instrument when image captures and analyses have been completed for the day If the Amersham Imager 600 instrument is used around the clock make sure to restart the system at least once a day to remove temporary files that may ...

Page 178: ... available as an independent software package Amersham Imager 600 Analysis Software can be installed on computers using Windows 7 or Win dows 8 1 operating system In this chapter See page Section 179 6 1 Software installation and activation 187 6 2 Software operation 178 Amersham Imager 600 Operating Instructions 29 0645 17 AD 6 Analysis software ...

Page 179: ...s performed in the following sequence 1 Install the Amersham Imager 600 Analysis Software 2 A registration number is presented by the Amersham Imager 600 Analysis Software 3 Go the main instrument to create a license key with the Amersham Imager 600 Control Software 4 Activate the Amersham Imager 600 Analysis Software by registering with the license key You need to have access to the main instrume...

Page 180: ...allShieldWizard dialog box appears Click Next 3 Read the license agreement If the license agreement is not acceptable please contact your GE representative 4 If the license agreement is acceptable select I accept the terms in the license agreement and click Next 5 180 Amersham Imager 600 Operating Instructions 29 0645 17 AD 6 Analysis software 6 1 Software installation and activation 6 1 1 Amersha...

Page 181: ...ine a suitable destination folder Click Next If C Program Files GE Healthcare Amersham Imager 600 Analysis Software already exists please save the software file to another location Otherwise the installer will ovrwrite the software file Click Install 7 Amersham Imager 600 Operating Instructions 29 0645 17 AD 181 6 Analysis software 6 1 Software installation and activation 6 1 1 Amersham Imager 600...

Page 182: ...ick Finish in InstallShield Wizard 8 182 Amersham Imager 600 Operating Instructions 29 0645 17 AD 6 Analysis software 6 1 Software installation and activation 6 1 1 Amersham Imager 600 Analysis Software installation ...

Page 183: ...he Reg istration dialogue box 1 Make a note of the 4 digit registration number for later use Note 3216 is an example This 4 digit number will differ each time you install the software 2 Note Access to the main instrument is needed for steps 3 7 Amersham Imager 600 Operating Instructions 29 0645 17 AD 183 6 Analysis software 6 1 Software installation and activation 6 1 2 Amersham Imager 600 Analysi...

Page 184: ...alysis software license appears Enter the Serial number and the Registration number Note The 6 digit serial number is printed on the analysis software DVD 4 184 Amersham Imager 600 Operating Instructions 29 0645 17 AD 6 Analysis software 6 1 Software installation and activation 6 1 2 Amersham Imager 600 Analysis Software registration ...

Page 185: ...ars 6 Result The 10 digit License key number is generated Make a note of the 10 digit License key number Amersham Imager 600 Operating Instructions 29 0645 17 AD 185 6 Analysis software 6 1 Software installation and activation 6 1 2 Amersham Imager 600 Analysis Software registration ...

Page 186: ...e registration of the analysis software is now complete Note The software license screen shows the number of licenses generated 186 Amersham Imager 600 Operating Instructions 29 0645 17 AD 6 Analysis software 6 1 Software installation and activation 6 1 2 Amersham Imager 600 Analysis Software registration ...

Page 187: ...is function in the Amersham Imager 600 Control Software Action Step The workflow starts with opening the image file and is followed by five steps of analysis The final result is presented in a summary Select Open in the start up screen to open images for analysis 1 Choose a folder containing the images to be analyzed 2 Note Up to 5 images can be opened simultaneously The image file opens in the an...

Page 188: ...art The current step in the analysis workflow is highlighted in blue 1 Use the Close button to close the analysis workflow 2 188 Amersham Imager 600 Operating Instructions 29 0645 17 AD 6 Analysis software 6 2 Software operation ...

Page 189: ...mage tools which modify display settings and to view image details 3 Display Zoom Contrast Intensity For more information see Image tools on page 145 Amersham Imager 600 Operating Instructions 29 0645 17 AD 189 6 Analysis software 6 2 Software operation ...

Page 190: ...h a blue frame 5 Options and steps associated with the current step in the analysis 6 Help icon Use to show additional information about the selected step in the analysis 7 Next button Use to proceed to the next step in the analysis workflow 8 190 Amersham Imager 600 Operating Instructions 29 0645 17 AD 6 Analysis software 6 2 Software operation ...

Page 191: ... grid to correspond with the lanes in the image Adjust the lane grid Follow the instructions below to adjust the lane grid to match lanes in the image Action Step Select the minus and plus buttons adjacent to Number of lanes to increase or decrease the number of grid lanes to correspond with the number of lanes in the image 1 Select a grid corner circle 2 Result The selected corner circle is highl...

Page 192: ...adjacent to Lane width to increase or decrease the lane fill factor Note If the value is 100 then the grid contains only the lanes Using a lesser per centage creates an empty space between lanes 4 When you are done select the Next button or select the Background button in the workflow The Previous button returns you to the captured image 5 192 Amersham Imager 600 Operating Instructions 29 0645 17 ...

Page 193: ...n option works This function shows the background level calculated by each option Tip The illustration and tables below describe the background subtraction options Description Option Use this option when no background should be subtracted from the image None Use this option to subtract the background below a baseline between the lowest points on the lane profile as though a rubber band was stretch...

Page 194: ...i mum profile Use this option to subtract the background below the graph as if erasing it using the radius of a ball A small radius will subtract more background than a large Rolling ball 194 Amersham Imager 600 Operating Instructions 29 0645 17 AD 6 Analysis software 6 2 Software operation 6 2 2 Background subtraction ...

Page 195: ...e current lane number displayed in the drop down list and the lane profile button in blue To confirm that each background subtraction options works check Show background check box This function shows the background level calculated by each option 2 Amersham Imager 600 Operating Instructions 29 0645 17 AD 195 6 Analysis software 6 2 Software operation 6 2 2 Background subtraction ...

Page 196: ...dius plus or minus buttons to set the radius of the virtual rolling ball eraser Tip The smaller the radius the more background will be removed Result The lane profile view is displayed with the background subtracted 196 Amersham Imager 600 Operating Instructions 29 0645 17 AD 6 Analysis software 6 2 Software operation 6 2 2 Background subtraction ...

Page 197: ...ber in the drop down menu 4 When ready press Next or select the Bands in the workflow Pressing Previ ous takes you back one step 5 Amersham Imager 600 Operating Instructions 29 0645 17 AD 197 6 Analysis software 6 2 Software operation 6 2 2 Background subtraction ...

Page 198: ...en channel is displayed with a green frame etc Detecting bands Follow the instructions below to detect bands Action Step Select the Detect button to detect the bands automatically 1 Tip Shift between lane profile image view and image tabs to verify the result of the band detection If the band detection is satisfactory then proceed to the final step in this table If too many bands are detected then...

Page 199: ...w buttons To adjust a band width select the band and adjust it with the Width plus and minus buttons To delete a band select the band and press Delete To delete all detected bands in the image select Clear Repeat actions in step 3 for other detection channels if relevant 4 When you are done detecting bands press the Next button or select the MW button in the workflow The Previous button will bring...

Page 200: ... weight calibration can only be performed in single channel view Note Action Step Select the lane containing the molecular weight marker 1 Result The selected lane appears highlighted Select the standard molecular weight marker from the drop down menu 2 If the correct marker is missing Add a missing marker by selecting Add new button See instructions below Edit a marker by selecting the Edit butto...

Page 201: ...lated molecular weight is presented in the Summary table Note Addamolecularweightstandard marker Action Step Select the Add new button 1 Result The following view appears Select the Name field and type the name of the marker 2 Select the unit from the Unit drop down menu 3 Type a value and select the Add button 4 Result The value will appear in the bottom of the list to the left on the screen Sele...

Page 202: ...n the list Note You may need to scroll to see the new value Select the Save button to save the edited values and to return to the MW Calibration view Select Cancel to leave without saving changes Delete will delete the entire marker group 4 To delete a value select the X next to the value Note 202 Amersham Imager 600 Operating Instructions 29 0645 17 AD 6 Analysis software 6 2 Software operation 6...

Page 203: ...lected band volumes Normalization is useful when comparing relative band volumes in different lanes The options for normalization are presented next to the image Normalize bands Follow the instructions below to perform normalization Action Step Select one or two control bands in a lane 1 Result The selected band s appear highlighted Note Control bands can only be selected in single channel view Wh...

Page 204: ...nter 3 Columns displaying 4 Ch the detection channel Lane the number of the lane change the sort order by selecting up or down arrow in the column heading Band the number of the band from top to bottom Volume the integrated signal intensity in the specific band Lane the relative band volume within a lane in percentage Normalized value the band volume relative to the control band volume Mw the calc...

Page 205: ...nts can be added to the file To overwrite the analysis of the file opened press Save 3 Result The image file is updated with the analysis information To create a new file set press Save as Result The Save image as view appears Amersham Imager 600 Operating Instructions 29 0645 17 AD 205 6 Analysis software 6 2 Software operation 6 2 6 Summary ...

Page 206: ...on to the image file s analyzed data is saved with a CSV file and the summary view is saved as a pdf file See Section 5 7 Save and manage image files on page 148 for details 206 Amersham Imager 600 Operating Instructions 29 0645 17 AD 6 Analysis software 6 2 Software operation 6 2 6 Summary ...

Page 207: ...rformed by service personnel authorized by GE Do not open any covers or replace parts unless specifically stated in the user documentation WARNING Do not use excessive amounts of liquids for cleaning the Amersham Imager 600 instrument This may result in instrument malfunction or electric shock NOTICE Whenperformingmaintenanceoftheequipment removethetablet computer There is risk of injury if it fal...

Page 208: ... or 70 ethanol then wipe with a new clean dry cloth To clean the interior of the instrument use a soft cloth slightly dampened with distilled water or 70 ethanol Make sure to wipe the interior dry Do not use any neutral domestic detergent as the detergent residue may fluoresce when an image is captured 208 Amersham Imager 600 Operating Instructions 29 0645 17 AD 7 Maintenance 7 1 Instrument ...

Page 209: ...id that residues fluoresce when images are captured The accessories may be washed with water and one of the following 70 ethanol Mild neutral detergent Isopropanol 6 H2O2 Cleaning sample trays white insert and diffuser board CAUTION Wear gloves while cleaning There may be residues of hazardous chemicals NOTICE Do not use organic solvents These may damage the surface Amersham Imager 600 Operating I...

Page 210: ...er using a soft sponge soaked in a cleaning agent After cleaning rinse the accessory immediately in water until no detergent remains and allow to air dry Cleaning gel sheets After use wash the gel sheet with mild detergent rinse with water then dry well A gel sheet can be reused about 20 times Note 210 Amersham Imager 600 Operating Instructions 29 0645 17 AD 7 Maintenance 7 2 Accessories ...

Page 211: ...plug is firmly secured in the power outlet The power cord and supply plug do not become overheated The power cord is not damaged in any way The ventilation holes in the equipment are free from dust and dirt Amersham Imager 600 Operating Instructions 29 0645 17 AD 211 7 Maintenance 7 3 Regular inspections ...

Page 212: ...ollow this procedure if an error occurs 1 Take note of the error code and error message on the monitor 2 Turn off the instrument by pressing the On Off button on the front panel and then the Power switch on the right hand side and turn it back on again after about ten seconds 3 If the error persists refer to this troubleshooting guide for possible solutions 4 If the error cannot be resolved with t...

Page 213: ...hen a camera head failure has occurred Turn off the power switch immediately and contact your GE representative Atypical odors are emitted then an instru ment failure has occurred Smoke is emitted Then If Turn off the power switch immediately and contact your GE representative Smoke is emitted from the camera head then a camera head failure has occurred Turn off the power switch immediately and co...

Page 214: ...losed or the door cannot be locked because a foreign object is present in the locking section or the door sensor The locking section is damaged Contact your GE representative The door to the instrument cannot be opened or closed or the door cannot be locked and no foreign object is present The ambient air temperature is too high Lower the room temperature to 28 C or lower the instrument cannot be ...

Page 215: ...he light source are dirty Change to the correct sample tray The wrong sample tray is used Select the correct light source The wrong light source is selected Place the sample correctly on the sample tray and place the tray in the correct tray position The size of the object exposed does not coin cide with the exposed area Light leaks on the image Then If Close the door to the instrument and expose ...

Page 216: ...rument and contact your GE representa tive A lamp in the UV transil luminator is broken Noise appears in an image exposed using the UV Trans tray Then If The UV Trans Tray is contaminated Clean the tray The UV Trans Tray is de teriorated from age Artefacts appear in a Colorimetric Epi image Then If Rotate the gel sample 90 positioning the gel so that the lanes of the gel are parallel to the long s...

Page 217: ...ion prop erly Check the sample position The sample is not in position The screen of Amersham Imager 600 is not displayed on the connected tablet computer Then If Contact the network administrator or connect a monitor mouse and keyboard directly with a cable Wireless access points are not properly set The buttons do not appear on an external monitor Then If Use monitors with SVGA resolution or high...

Page 218: ...ew minutes for the instrument to come online The wireless network can be detected before the instrumented is fully connected Normally the instrument becomes online within 5 minutes Restart the software on the tablet com puter Press the home button on the tablet computer and restart the software The software display on the tablet com puter screen has frozen after a long time of inactivity Use gentl...

Page 219: ... page to the home screen of the tablet computer by clicking on the square with an upward arrow next to the address field in the browser Tip If a USB dongle is used on the camera the default IP address is 192 168 173 1 TheAmershamImager600iconhasbeen accidently removed from the tablet computer Amersham Imager 600 Operating Instructions 29 0645 17 AD 219 8 Troubleshooting 8 3 Problems with the softw...

Page 220: ...e Green Epi light 520 nm Red Epi light 630 nm UV transillumination light 312 nm White Epi light 470 to 635 nm White transillumination light 470 to 635 nm Fully automated auto exposure no focus or other adjustment or calibrations needed Operation 160 X 220 mm Maximum sample size 65 536 levels 16 bit Greyscale 1 100 second to 12 hours Exposure time 4 8 orders of magnitude Dynamic range Binning crop ...

Page 221: ...supply Data Parameter 100 to 240 V AC Input voltage 10 Voltage variation 50 60 Hz Frequency 250 W Max power Environmental conditions The following table describes the environmental requirements for Amersham Imager 600 CAUTION Do not use the instrument in a room with a temperature above 28 C Higher temperatures does not allow the CCD to cool down properly to 25 C Amersham Imager 600 Operating Instr...

Page 222: ...her environmental require ments have been met 4 Do not install the equipmentnear a window Avoid direct sunlight Ensure blinds are attached to nearby windows 5 Do not place objects near the power outlet to ensure easy access to the power cord for disconnection in case of emergency Other conditions Temperature 18 C to 28 C with temperature fluctuation below 10ºC per hour or lower Operatingtemperatur...

Page 223: ... dusty environment 6 in a place constantly or excessively exposed to vibration or im pacts 7 in a place exposed to direct sunlight Indoor use Operation site 2000 m or lower Maximum operating altitude 70 dB A or lower Noise Fast Lmax 60dB A and average Leq 54dB A 1 m from the instru ment IP21 Protectionprovidedbyenclosure Transient overvoltage category II Overvoltage category Pollution degree 2 Rat...

Page 224: ...00 208 Sample trays and filter 210 Manufacturing information 7 Monitor requirements 19 Moving instrument 65 Multichannel imaging 124 N Notes and tips 6 O Operation Place the sample 90 Sample and tray combina tions 89 Save the image 148 P Place the sample 90 Print Printer 48 Purpose of this document 4 R Recycling information 26 Regulatory compliance 9 S Safety notices 5 Safety precautions 12 Instal...

Page 225: ...ypad 56 Specifications 220 Start screen 41 T Tool Web 81 Tools Contrast tool 146 Display tool 145 Intensity tool 147 Zoom tool 145 Troubleshooting 212 Two channel imaging 124 Typographical conventions 6 U User information important 5 V Virus protection 75 W Web tool 81 Windows 7 79 Z Zoom tool 145 Amersham Imager 600 Operating Instructions 29 0645 17 AD 225 Index ...

Page 226: ...subject to the terms and conditions of sale of the company within GE Healthcare which supplies them A copy of these terms and conditions is available on request Contact your local GE Healthcare repre sentative for the most current information GE Healthcare Europe GmbH Munzinger Strasse 5 D 79111 Freiburg Germany GE Healthcare UK Limited Amersham Place Little Chalfont Buckinghamshire HP7 9NA UK GE ...