Chemiluminescence exposure
options
There are five exposure options for Chemiluminescence capturing. The table below
suggests the options to use in different situations.
Then...
When...
Select Auto for automatic exposure.
An optimal dynamic
range is required:
A short pre-exposure is performed to determine the signal
intensity. The system will use this information to calculate
which exposure time will give the highest possible signal
below saturation to enable accurate quantification of the
sample.
To use the full dynamic range of the image file, check High
dynamic range.
To minimize exposure time, leave High dynamic range
unchecked.
Select Semi-automatic.
A specific area of a
sample is of interest:
A short pre-exposure is performed which results in an
image. This allows you to select the area of the sample
image to focus on. The software automatically calculates
the optimal exposure time for the specified area.
To use the full dynamic range of the image file, check High
dynamic range.
To minimize exposure time, leave High dynamic range
unchecked.
112
Amersham Imager 680 Operating Instructions 29271308 AB
5 Operation
5.5 Capture an image
5.5.2 Chemiluminescence capture
