Intended use
Configuration
Fluorescence with five lasers, phosphor
imaging, and densitometry
Amersham Typhoon scanner 5
Fluorescence with two lasers, 685 nm
and 785 nm, for NIR fluorescence appli-
cations.
Amersham Typhoon scanner NIR
For an overview of the types of lasers that are installed, refer to
Amersham Typhoon scanner, on page 132
and
Laser specifications, on page 132
.
Scanning modes
The Amersham Typhoon scanner can be used for scanning with three different scanning
modes.
Used for
Mode
•
Scanning fluorescent samples using different combina-
tions of lasers and filters.
•
Scanning luminescent samples, with all the lasers
turned off and no filters in use.
Fluorescence
Scanning storage phosphor screens that have been ex-
posed to radioactive samples, to image and quantify radi-
oligands in the samples.
Phosphor imaging
•
Scanning gels to obtain digital images of gels for doc-
umentation.
•
Densitometric imaging of stained gels (e.g., Coom-
massie-stained gels) for quantitation of samples.
Densitometry
Photomultiplier tubes
The photomultiplier tube (PMT) detects light and converts it to an electric signal. The PMT
potential (in volt) determines the amplitude of the signal.
Different types of photomultiplier tubes are installed in the Amersham Typhoon scanner:
•
Bi-alkali
•
Multi-alkali
Amersham Typhoon Operating Instructions 29193226 AC
41
3 System description
3.1 System overview