GE EPS 3501 XL, Operating Instructions Manual

Page 1: ...GE Healthcare Electrophoresis Power Supply EPS 3501 XL Operating Instructions Original instructions...

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Page 3: ...eatures 20 2 5 Recycling information 21 3 Installation 23 3 1 Site requirements 23 3 2 Unpacking 23 3 3 Transport 23 3 4 Connections 24 3 5 Spare parts and accessories 25 4 Operation 27 4 1 Operation...

Page 4: ...Table of Contents 4 Electrophoresis Power Supply EPS 3501 XL Operating Instructions 28 9639 69 AA...

Page 5: ...nderstand the concepts of electrophoresis You must read the Safety Instructions in Chapter 2 of these Operating Instructions The system should be installed according to the instructions in Chapter 3 o...

Page 6: ...ting Instructions contain WARNINGS CAUTIONS and NOTICES concerning the use of the product with meanings as defined below WARNING WARNING indicates a hazardous situation which if not avoided could resu...

Page 7: ...s e g File Open refers to the Open option in the File menu 1 2 Regulatory information This section lists the directives and standards that are fulfilled by EPS 3501 XL Manufacturing information CE Con...

Page 8: ...lthcare Regulatory compliance of connected equipment Any equipment connected to EPS 3501 XL should meet the safety requirements of EN 61010 1 IEC61010 1 or relevant harmonized standards Within the Eur...

Page 9: ...electric focusing using Immobiline or carrier ampholytes such as CleanGel and ExcelGel Flatbed SDS PAGE Polyacrylamide Gel Electrophoresis or flatbed native PAGE EPS 3501 XL is also suitable for DNA s...

Page 10: ...ment 10 Electrophoresis Power Supply EPS 3501 XL Operating Instructions 28 9639 69 AA Instrument description Figure 1 1 The main parts of the EPS 3501 XL instrument 1 3 2 Part Description 1 Display 2...

Page 11: ...tage is applied HV on and connectors for two electrophoresis units Display Indicator Switch Color Description HV ON indicator lamp Green The HV ON lamp indicates that the high voltage power supply is...

Page 12: ...n time and volthours in RUN PAUSE and END The values scroll i e they automatically change from maximum to minimum value or vice versa run Press run to start the run and put the program into RUN mode T...

Page 13: ...am in MORE mode This gives access to some special functions These include COPY Copying a program CLEAR Clearing a program SETUP Disabling the start current check MORE mode cannot be activated in RUN o...

Page 14: ...1 Introduction 1 3 Instrument 14 Electrophoresis Power Supply EPS 3501 XL Operating Instructions 28 9639 69 AA...

Page 15: ...g the equipment you must be aware of the hazards described in this manual Follow the instructions provided to avoid personal injury or damage to the equipment The safety precautions in this section ar...

Page 16: ...performed by properly trained personnel only WARNING EPS 3501 XL is a high voltage instrument that can cause fatal electrical shock if the safety features are disabled The safety lid must be securely...

Page 17: ...power cord must always be easy to disconnect WARNING Hazardous biological agents during run When using hazardous biological agents flush the entire system tubing with bacteriostatic solution followed...

Page 18: ...sed in safety labels 28 9269 30 ETTAN IPGphor 3 Made in Sweden 28952336aa GE Healthcare Bio Sciences AB 751 84 Uppsala Sweden Serial No 1234567 N12406 Code No 11003364 Mfg Year 2009 Voltage 100 240 V...

Page 19: ...le European directives Label Meaning Label Meaning This symbol indicates that the waste of electrical and electronic equipment must not be disposed as unsorted municipal waste and must be collected se...

Page 20: ...required disconnect the mains power cord The run is interrupted immediately Power failure to will result in EPS 3501 XL The run is interrupted immediately in an undefined state Function Description F...

Page 21: ...501 XL contains hazardous substances Detailed information is available from your GE Healthcare representative Disposal of electrical components Waste of electrical and electronic equipment must not be...

Page 22: ...2 Safety instructions 2 5 Recycling information 22 Electrophoresis Power Supply EPS 3501 XL Operating Instructions 28 9639 69 AA...

Page 23: ...on see Electrophoresis Power Supply EPS 3501 XL User Manual 3 1 Site requirements 3 2 Unpacking Check the equipment for damage before starting assembly and installation Document any damage and contact...

Page 24: ...nel Local regulation for Great Britain This appliance must be earthed The wires in the mains lead are colored in accordance with the following code If the plug provided is unsuitable for your socket o...

Page 25: ...d black The brown wire must be connected to the terminal which is marked with the letter L or colored red Note After replacing or changing a fuse the fuse cover in the plug must be replaced with a fus...

Page 26: ...3 Installation 3 5 Spare parts and accessories 26 Electrophoresis Power Supply EPS 3501 XL Operating Instructions 28 9639 69 AA...

Page 27: ...racters in the display are shown as bold characters in this manual 4 2 Starting the instrument Turn on the instrument by pressing the Power switch to the I position When the power supply is turned on...

Page 28: ...h 1 1 enter set 2 1 2 1 0 mA 0 W 2 1 0 mA 0 W 1 mA 5 W 0 00 h 2 1 enter set enter set SET 1000 V 10 mA 0 W 0 00 h 2 1 enter set enter set Vh SET 1000 V 10 mA 10 W 0 00 h 2 1 SET 1000 V 10 mA 10 W 0 V...

Page 29: ...led by one of these limiting values which means that it is run at either constant voltage current or power The EPS 3501 XL automatically switches over the controlling parameter according to programmed...

Page 30: ...a step program Choosing gradient means that a voltage endpoint for the actual phase is programmed together with current and power limiting values A linear voltage gradient is made with zero for the f...

Page 31: ...radient programming the programming for the IEF part of a 2 D electrophoresis with Immobiline DryStrip Gels is shown in Table 4 2 and Figure 4 3 Note that the first phase is a very steep gradient to r...

Page 32: ...or manual break choose OFF with Confirm with set enter Note that the breakpoint unit is valid for all phases within the program and you will only get this question when programming the first phase see...

Page 33: ...with the program number flashing It is possible to go back to this position at any stage during programming in SET mode by pressing exit or STOP Note that the program is automatically saved with all c...

Page 34: ...e is done by entering the last phase as above moving to the question Last Phase and answering No N The program will jump to the start position for programming a phase with the phase number blinking Pr...

Page 35: ...Choosing a program Press set enter and select the program you wish to run by pressing until the value is correct Omit this step if you have just programmed or edited a method as described in Programmi...

Page 36: ...Insert delete 4 Choose Insert or Delete 5a Program a new phase 2 5b Back to new Phase 2 start position Old phase 2 is deleted step GRADIENT enter set A 500 V 1 mA 5 W SET 1 1 1 Vh SET 2 1 SET 2 1 100...

Page 37: ...omplete press either pause continue again or run to continue the run from where it was interrupted Editing a running program when in PAUSE When in the PAUSE mode you can also press set enter to make c...

Page 38: ...umber 2 Start the run 3 During a run you can view the settings It is also possible to interrupt the run and make changes in the program 4 Stop the run manually 5 The run is stopped automatically 1000...

Page 39: ...o to zero as indicated by the HV on LED switching off The end parameter values are displayed Switch between total elapsed time integrated voltage or integrated current for all phases in the program by...

Page 40: ...4 Operation 4 5 Performing a run 40 Electrophoresis Power Supply EPS 3501 XL Operating Instructions 28 9639 69 AA...

Page 41: ...upply EPS 3501 XL NOTICE Cleaning Keep the instrument dry and clean Wipe regularly with a soft damp tissue and if necessary a mild cleaning agent Let the instrument dry completely before use WARNING E...

Page 42: ...5 Maintenance 5 1 General 42 Electrophoresis Power Supply EPS 3501 XL Operating Instructions 28 9639 69 AA...

Page 43: ...Cause Action The current is less than the lower limit This can be due to incorrect connection of the electrophoresis equipment or due to use of buffers with extremely low conductivity 1 Check connecti...

Page 44: ...6 Troubleshooting 44 Electrophoresis Power Supply EPS 3501 XL Operating Instructions 28 9639 69 AA...

Page 45: ...XL refer to the Electrophoresis Power Supply EPS 3501 XL User Manual 7 3 Ordering information For ordering information visit www gelifesciences com 2DE Parameter Value Ingression protection IP20 Supp...

Page 46: ...7 Reference information 7 3 Ordering information 46 Electrophoresis Power Supply EPS 3501 XL Operating Instructions 28 9639 69 AA...

Page 47: ...al and communication connections Appendix A Appendix A Electrical and communication connections Voltage 100 120 220 240 V AC Frequency 50 60 Hz Power 260 W 4 AMax 100 120 220 240 MAINS Disconnectbefor...

Page 48: ...Appendix A Electrical and communication connections 48 Electrophoresis Power Supply EPS 3501 XL Operating Instructions 28 9639 69 AA...

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Page 50: ...009 All goods and services are sold subject to the terms and conditions of sale of the company within GE Healthcare which supplies them A copy of these terms and conditions is available on request Con...