Operating instructions
3
Ettan IPGphor 3 Instrument User Manual 11-0034-58 Edition AB
37
3.5
Creating, editing, and running protocol programs
from the Ettan IPGphor 3 control panel
3.5.1
Protocol parameters
The Ettan IPGphor 3 can store up to ten protocols. Programmable protocol
parameters include:
•
duration and temperature of the rehydration phase
•
duration, temperature, and power parameters for up to nine IEF steps for
each protocol
•
power parameters including maximum current, voltage, and voltage
change pattern.
Please refer to the
2-D Electrophoresis, principles and methods guide
for sample
handling recommendations and protocol guidelines. The permitted range of
values for each programmable parameter is listed below.
Navigating parameter “fields” and “digits”:
•
Each screen consists of information as well as one or more fields that can be
edited. The active fields contain individually adjustable digits or characters.
•
Certain digits are also linked to their neighbouring digits. For example, three
of the four digits in the voltage field can each be adjusted individually, but
when adjusting the value from 1000 to 990, for instance, even though only
the ten’s place is adjusted manually, three digits are affected.
•
Whether individual or linked, digits “wrap around,” so increasing from 9
produces 0 and decreasing from 0 produces 9—until a parameter limit is
reached.
The Ettan IPGphor 3 can be programmed in volt-hours (Vhr) or hours and minutes
in step-n-hold or in gradient mode. Any values within the operating limits listed
above can be used.
WARNING!
Check that the high voltage is turned off before opening the safety
lid. The HV ON indicator on the front panel should not light.
Parameter
Operational range
Recommended values
Steps
1–9
Step duration (hrs)
rehydration: 0–99:59
focusing: 0–99:59
10:00–14:00
2:00–10:00
Volts (V)
0–10000
100–10000
Max. current
1500 µA total for 12 strips max. 50–75 µA per strip
Platform temperature (°C) rehydration: 15–30
focusing: 15–30
20
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Summary of Contents for Ettan IPGphor 3
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