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GE Filter Kit HR 10, Instructions Manual

The GE Filter Kit HR 10 comes with a comprehensive Instructions Manual that ensures easy product installation and maintenance. This essential manual is available for free download on our website, conveniently accessible to all users. Never go without the necessary instructions to maximize the benefits of this superb filter kit.

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GE Healthcare
Life Sciences

Instructions 52-1777-00 AM

Filter Kit HR10/HR16

Filter Kit HR 10, Filter Kit HR 16

These instructions describe how to change filters and O-rings on
HR 10 and HR 16 columns.

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Summary of Contents for Filter Kit HR 10

Page 1: ...GE Healthcare Life Sciences Instructions 52 1777 00 AM Filter Kit HR10 HR16 Filter Kit HR 10 Filter Kit HR 16 These instructions describe how to change filters and O rings on HR 10 and HR 16 columns ...

Page 2: ...Table of Contents 3 1 Filter kit contents 3 2 When to change the filter 3 3 How to change the filter 6 4 Troubleshooting 2 52 1777 00 AM ...

Page 3: ...hanging the bottom filter may lead to loss of efficiency 3 How to change the filter To change the filter you need a Filter tool Code No 18 3590 01 consisting of a hook and a plunger Action Step Start the pump and pump buffer solution e g the buffer previously used through the column at a flow rate of 1 to 1 5 ml min Note Do not exceed recommended maximum backpressure 1 Gradually rotate the red adj...

Page 4: ...w filter in 20 ethanol Put the filter into the column avoiding air bubbles under it Push it gently down to the medi um surface with the plunger For HR 16 columns use the wider end of the plunger Note As the filter is inserted any medium which has been stirred up will be packed onto the top of the bed 7 Make sure the filter is horizontal and remove the plunger 8 Remove the liquid and traces of medi...

Page 5: ...edium and the adapter If a large air bubble has become trapped carefully remove and replace the adapter 13 Connect the column inlet tubing to the valve 14 Start the pump and pump buffer at a flow rate of 1 to 1 5 ml min for 5 to 10 minutes to pack the top of the medium bed 15 Stop the pump and disconnect the column inlet from the valve 16 Adjust the adapter to the filter again by clockwise rotatio...

Page 6: ...t re suspend 2 3 mm of the top of the medium bed using the plunger as described above Step 6 Remove the suspension and replace the filter and adapter As the final attempt to restore performance check carefully that the adapter is properly adjusted to the medium bed and reverse the flow direction in the column Use a flow rate of 1 to 1 5 ml min for 10 to 30 minutes For further information contact y...

Page 7: ...conditions of sale of the company within GE Healthcare which supplies them A copy of these terms and conditions is available on request Contact your local GE Healthcare representative for the most current information GE Healthcare Europe GmbH Munzinger Strasse 5 D 79111 Freiburg Germany GE Healthcare UK Limited Amersham Place Little Chalfont Buckinghamshire HP7 9NA UK GE Healthcare Bio Sciences Co...

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