Action
Step
Carefully and slowly bring up the loose
filter by using the hook (part of the Filter
tool). Do not press or puncture the medi-
um bed.
Note:
Only a very small amount of medium
should be in suspension after removing the
filter.
5
Check that the surface of the medium bed
is horizontal and even. If not, use the
plunger (part of the Filter tool) and careful-
ly stir up 2–3 mm of medium by rotating
it on the very top of the medium bed.
6
Wet a new filter in 20% ethanol. Put the
filter into the column, avoiding air bubbles
under it. Push it gently down to the medi-
um surface with the plunger. For HR 16
columns, use the wider end of the plunger.
Note:
As the filter is inserted, any medium which
has been stirred up will be packed onto the
top of the bed.
7
Make sure the filter is horizontal, and remove the plunger.
8
Remove the liquid and traces of medium above the filter with
a pasteur-pipette. Rinse the walls and the top of the filter with
small aliquots of buffer until all the medium particles above
the filter have been removed.
9
Fill the volume above the filter with buffer.
10
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