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PD MiniTrap G-25 

Product Booklet

Code:   28922529

PD MiniTrap G-25 contains

•  50 prepacked disposable PD MiniTrap™ columns containing 
 

2.1 mL of Sephadex™ G-25 resin 

•  4 adapters 
•  Instructions for use

Purpose

PD MiniTrap G-25 columns are prepacked and designed for 
rapid, convenient sample clean-up of proteins and other large 
biomolecules (>5000 M

r

).

PD MiniTrap G-25 columns can be used in a wide range of 
applications such as desalting, buffer exchange and removal of  
low-molecular weight compounds

Summary of Contents for PD MiniTrap G-25

Page 1: ...adex G 25 resin 4 adapters Instructions for use Purpose PD MiniTrap G 25 columns are prepacked and designed for rapid convenient sample clean up of proteins and other large biomolecules 5000 Mr PD Min...

Page 2: ...ntain Sephadex G 25 resin which allows rapid group separation of high molecular weight substances from low molecular weight substances PD MiniTrap G 25 columns are used for desalting buffer exchange a...

Page 3: ...Group separation can be made using two different protocols gravity protocol and spin protocol GE provides an assortment of sample clean up products The different formats available are summarized in Ta...

Page 4: ...diluted Spin protocol Additional gravity force is added by spinning the column in a centrifuge for some protocol steps There is no dilution of the sample Table 2 Protocol overview Protocol Sample vol...

Page 5: ...lumes less than 0 5 mL allow the sample to enter the packed bed completely and then add equilibration buffer stacker volume so that the total volume of sample and buffer added equals 0 5 mL Allow the...

Page 6: ...CP biocide is potentially allergenic Use gloves when discarding the storage solution 4 Column assembly For use in the spin protocol the PD MiniTrap G 25 column should be assembled with adapter and col...

Page 7: ...ilibration buffer should be used in total for all three steps 3 Sample application Add maximum 0 5 mL of sample to the column For sample volumes less than 0 5 mL add equilibration buffer to adjust the...

Page 8: ...and al low the equilibration buffer to enter the packed bed completely Repeat once Discard the flow through Fill up the column a third time with equilibration buffer and spin down at 1000 g for 2 min...

Page 9: ...f bovine serum albumin 1000 g mL in 1M NaCl was applied onto the column The protein recovery was 95 and the desalting capacity was above 99 see Fig 2 Fig 2 Removal of NaCl from albumin solution with P...

Page 10: ...7 2 8 cm 2 1 mL Maximum sample volume 0 5 mL Volume of eluted sample gravity 1 0 mL Volume of eluted sample spin 0 5 mL Desalting Capacity 90 Exclusion limit Mr 5000 Chemical stability All commonly us...

Page 11: ...04 PD MultiTrap G 25 4 96 well filter plates 28 9180 06 PD MidiTrap G 25 50 28 9180 08 PD MiniTrap G 10 50 28 9180 10 PD MidiTrap G 10 50 28 9180 11 MiniSpin Adapter 10 28 9232 43 HiTrap Desalting 5...

Page 12: ...sold subject to the terms and conditions of sale of the company within GE Healthcare which supplies them A copy of these terms and conditions is available on request Contact your local GE Healthcare r...

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