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4. Operation of DEN-1

Recommendation during operation

Keeping the unit switched on for 15 min before starting the operation for the operation mode, 

stabilisation is recommended.

If flat-bottomed tubes are used, the solution level should be higher than 7 mm from a tube bottom; 

if round-bottomed tubes are used, the level should be higher than 12 mm from a tube bottom.

4.1.

Connect the external power supply unit to electric circuit.

4.2.

Switch ON the unit using the Power switch (Fig.1/

u

) on the rear panel (position I).

4.3.

The following indication may be shown on the display (fig.2/

v

):

“0.0” means that the unit is calibrated and ready for operation (if no any tube inserted);
“CC” (flashing) means that the unit is not calibrated. Calibrate the unit.
“EE” means that operator error message. Switch OFF the unit and then switch ON again. 

4.4.

Shake the tube with the solution (it is recommended to use a vortex mixer, e.g. Vortexer PV-1, for 

shaking) and insert it into the socket (fig.2/

u

). The McFarland value for the solution will be 

shown on the display (fig.2/

v

).

4.5.  Glass and transparent plastic tubes (16 or 18 mm in external diameter) can be used for work with 

DEN-1 densitometer. An adapter must be used for work with tubes which are 16 mm in external 
diameter. 

Note! 

The unit must be calibrated each time a tube type (e.g. with different outer diameter, 

bottom shape or different material [transparent plastic tubes]) is changed.

4.6.

After finishing the operation switch OFF the unit using the Power switch (position O). Disconnect 

the power supply from electric circuit.

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Fig.2 Front panel

Page 7

DEN-1
Operating instructions

Version 1.03 - July 2013

Summary of Contents for DEN-1

Page 1: ...Densitometer DEN 1 Operating instructions For version V 1GW ...

Page 2: ...tents 1 Safety 3 2 General Information 4 3 Getting started 5 4 Operation of DEN 1 7 5 Maintenance 9 6 Specifications 10 7 Guarantee and service 11 Page 2 DEN 1 Operating instructions Version 1 03 July 2013 ...

Page 3: ...this product Ensure that the power switch and external power supply unit are easily accessible during use Disconnect the unit from the external power supply unit before moving If liquid penetrates into the unit disconnect it from the external power supply unit and have it checked by a repair and maintenance technician DURING OPERATION Do not operate the unit in environments with aggressive or expl...

Page 4: ...ards e g produced by bioMerieux Lachema etc and the standards prepared in the laboratory can be used for calibration Table 1 shows the data provided by the McFarland standard manufacturer bioMerieux Table 1 Interpretation of McFarland Standard results into corresponding numeric values of bacterial suspension concentration and their optical density at 550 nm Bacterial concentration depends on micro...

Page 5: ...y Optional accessories DENMCF STDS calibration kit for glass tubes 16 mm in diameter on request DENMCF 18 STDS calibration kit for glass tubes 18 mm in diameter on request 3 3 Set up Place the unit on the horizontal even working surface Plug the external power supply unit into the 12 V socket at the rear side of the unit fig 1 v 3 4 Calibration The device is pre calibrated at the factory for opera...

Page 6: ...rd value 5 0 3 4 8 After finishing the calibration switch OFF the unit using the Power switch position O Disconnect the external power supply unit from electric circuit 3 5 An adapter D16 must be used for work with tubes which have external diameter 16 mm Insert the adapter into the tube socket fig 2 v perform calibration Use at least 2 points for calibration Note Shake the tube with the standard ...

Page 7: ...ibrated Calibrate the unit EE means that operator error message Switch OFF the unit and then switch ON again 4 4 Shake the tube with the solution it is recommended to use a vortex mixer e g Vortexer PV 1 for shaking and insert it into the socket fig 2 u The McFarland value for the solution will be shown on the display fig 2 v 4 5 Glass and transparent plastic tubes 16 or 18 mm in external diameter...

Page 8: ...ng method except that recommended check with our Service Department or in other countries with our distributor that the proposed method will not damage the equipment The cases can be cleaned with a damp cloth after disconnection Page 8 DEN 1 Operating instructions Version 1 03 July 2013 ...

Page 9: ...xternal diameter of tube 18 mm or 16 mm when using D16 adapter Display LED Dimensions 165x115x75 mm Input current power consumption 12 V 80 mA 1 W External power supply unit input AC 100 240 V 50 60 Hz output DC 12 V Weight 0 7 kg Accurate within 10 Grant is committed to a continuous programme of improvement specifications may be changed without notice Page 9 DEN 1 Operating instructions Descripti...

Page 10: ...tions this product is guaranteed for TWO YEARS against faulty materials or workmanship 7 2 Service For service return for repair to our Service Department in the UK or in other countries to our distributor 7 Guarantee and Service Page 10 DEN 1 Operating instructions Version 1 03 July 2013 ...

Page 11: ...DEN 1 Operating instructions Page 11 Version 1 03 July 2013 ...

Page 12: ...nsitometer DEN 1 28712 1 03 Grant Instruments Cambridge Ltd Shepreth Cambridgeshire SG8 6GB UK Tel 44 0 1763 260811 Fax 44 0 1763 262410 Email scientificsales grantinstruments com www grantinstruments com ...

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