Grant-bio RTS-1 Operating Instructions Manual Download | Manualshive

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Personal Bioreactor

RTS-1 & RTS-1C

Operating instructions

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Summary of Contents for RTS-1

Page 1: ...Personal Bioreactor RTS 1 RTS 1C Operating instructions ...

Page 2: ...Edition 3 4 03 July 2017 Personal bioreactor RTS 1 RTS 1C Page 2 Operating instructions ...

Page 3: ...alibration coefficients 600nm 850nm 10 8 5 Factory calibration growth phase influence on measurement accuracy 10 8 6 Conversion rate coefficient of user calibration 10 9 Temperature control 11 9 1 Rotation intensity influence on temperature accuracy of the sample 11 9 2 Dew point temperature influence on measurement system accuracy 11 9 3 Cold room and environmental chamber influence on temperatur...

Page 4: ...mber label Use only the external power supply provided with this product Ensure that the external power supply and switch are easily accessible during use Disconnect the unit from the electric circuit before moving Turn off the unit by switching off the power switch and disconnecting the external power supply from the power socket If liquid penetrates into the unit disconnect it from the external ...

Page 5: ...ering of the sample in real time without probe interference maintaining process sterility Changing the parameters such as temperature revolution per minute and period of spinning in one direction together with pos sibility of creating experiment algorithms including temperature profiling mixing intensity profiling optical density control etc allows both performance of difficult sequence of algorit...

Page 6: ...ware following the software installation procedure de scribed in software installation manual 4 4 Bioreactor vessel features Falcon type tubes TPP TubeSpin Bioreactor Working volume 10 30 ml Conical form 5 openings A B C D E of different size above the gas permeable sterile PTFE filter of the screw cap Openings can be sealed and by this exchange adjusted to need Sterile gas exchange is guaranteed ...

Page 7: ...ossibility to reset baseline value for device maintenance 5 3 1 Get cell suspension samples in falcon tubes with typical optical densities of your experiments If the maximal OD of your experi ment stationary phase is 5 OD600nm then the recommended samples are 0 ddH2O water or broth media 1 2 3 4 5 6 OD600nm Volume accuracy of the samples must be 0 05 Measure OD at desired wavelength of each cell s...

Page 8: ...he tube into the socket fig 1 4 Attention Data obtained in manual mode has a rounded and referential value 6 4 Software control mode Switch on the computer with installed software and continue working according to software operation manual 6 5 Manual mode 6 5 1 Press the Select key fig 2 1 to choose a parameter that you want to change the active parameter is blinking 6 5 2 Use and keys fig 2 2 to ...

Page 9: ...esophiles II Thermophiles III thermotolerant 3 facultative 4 obligate 5 and extremophile 6 Thick line mark represents optimal growth temperature Figure 4 Temperature boarders and optimal growth zones of prokaryotes and their classification 8 1 1 For psychrophiles that are cultivated at temperatures of 15 C 2 C below ambient the device must be installed in a cold room or a refrigerated chamber Desp...

Page 10: ...icroorganism size of 0 4 0 8 x 1 3 μm and a cell volume of approx imately 0 5 5 0 μm3 In case of exceeding the allowable size the measurement system will not work correctly Optical density ODλ 850 nm to ODλ 600 nm conversion rate coefficient is equal to 1 9 cells taken for measurement from stationary phase using a spectrophotometer and 1mm optical path cuvette Example of calculation to convert 3 5...

Page 11: ...nd point on the graph is the temperature of the dew point 9 2 2 Avoiding dew point temperature during temperature profiling If the dew point temperature reached moisture will disrupt correct measurement of the system To avoid dew point temperature decrease the temperature difference between the ambient temperature and bioreactor and sam ple temperature By placing the bioreactor to an environmental...

Page 12: ...30 ml volume 0 8 0 15 2 ODλ600 nm equivalent Measurement Precision OD 0 3 Real time measurement measurement h 1 60 Time setting resolution min 1 10 2 Temperature specifications Setting range C RTS 1 25 70 RTS 1C 4 70 Bottom control range point C RTS 1 5 above ambient RTS 1C 15 below ambient Top control range point C 70 Setting resolution C 0 1 Stability C 0 1 Sample temperature accuracy C 20 C 45 ...

Page 13: ...possible contamination after finishing fermentation 12 Device calibration for maintenance Note Only use this calibration after hardware re installation Following instructions apply to Resetting baseline value for factory hardware pre calibrated devices with hardware version 8 or earlier Adjusting RPM fluctuation for signal strength stabilization which is required for maintenance after re installat...

Page 14: ...1 2013 Electrical equipment for measurement control and laboratory use EMC requirements General requirements LVS EN 61010 1 2011 Safety requirements for electrical equipment for measurement control and laboratory use General requirements LVS EN 61010 2 010 2015 Particular requirements for laboratory equipment for the heating of materials LVS EN 61010 2 051 2015 Particular requirements for laborato...

Page 15: ...Personal bioreactor RTS 1 RTS 1C Edition 3 4 03 July 2017 Operating instructions Page 15 ...

Page 16: ...ng the details below UK Office Keison Products P O Box 2124 Chelmsford Essex CM1 3UP England Tel 44 0 330 088 0560 Fax 44 0 1245 808399 Email sales keison co uk Please note Product designs and specifications are subject to change without notice The user is responsible for determining the suitability of this product ...

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