CRF-1 Operating Manual
Page 44 of 60
33801-V4 DMN S90
8. Freezing Profiles
The CRF-1 series freezers are pre-loaded with specific cooling profiles.
8.1. Embryo Cryopreservation
Profile name: Embryo
Applicable model H00, H01, H03, H07, H08
Start temperature
20°C
Cool
from 20°C to -7°C at 2°C min
-1
Hold for nucleation
at -7°C for 10 minutes
Cool
from -7°C to -30°C at 0.3°C min
-1
Cool
from -30°C to -33°C at 3°C min
-1
Cool
from -33°C to -39°C at 6°C min
-1
Cool
from -39°C to -100°C at 10°C min
-1
Hold
at -100°C
Transfer to storage
Figure 47: Pre-programmed embryo freezing cycle
-110
-100
-90
-80
-70
-60
-50
-40
-30
-20
-10
0
10
20
30
0
10
20
30
40
50
60
70
80
90 100 110 120 130 140 150 160 170 180
Time (mins)
Temperature (
degC)
Summary of Contents for CRF-1 H00
Page 1: ...Controlled Rate Freezer CRF 1 Series Operating Manual...
Page 59: ......
