CRF-1 Operating Manual
Page 46 of 60
33801-V4 DMN S90
8.4. Embryonic Stem Cell Cryopreservation
Profile name: Stem Cell
Applicable models: H00, H01, H03, H07, H08
Start temperature
20°C
Cool
from 20°C to -7°C at 2°C min
-1
Hold for nucleation
at -7°C for 10 minutes
Cool
from -7°C to -80°C at 1°C min
-1
Hold
at -80°C
Transfer to storage
Figure 50: Pre-programmed embryonic stem cell freezing cycle
8.5. Spermatozoa Cryopreservation
– Linear Cooling
Profile name: Sperm
Applicable models: H00, H01, H03, H07, H08
Start temperature
20°C
Cool
from 20°C to -7°C at 2°C min
-1
Hold for nucleation
at -7°C for 10 minutes
Cool
from -7°C to -100°C at 10°C min
-1
Hold
at -100°C
Transfer to storage
Figure 51: Pre-programmed sperm - linear freezing cycle
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Temperature (
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Time (mins)
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Summary of Contents for CRF-1 H00
Page 1: ...Controlled Rate Freezer CRF 1 Series Operating Manual...
Page 59: ......
