CRF-1 Operating Manual
Page 47 of 60
33801-V4 DMN S90
8.6. Oocyte
– Non-Linear Cooling Cryopreservation
Profile name: OocyteNL
Applicable models: H00, H01, H03, H07, H08
Start temperature
20°C
Cool
from 20°C to -7°C at 2°C min
-1
Hold for nucleation
at -7°C for 10 minutes
Cool
from -7°C to -18.1°C at 0.5°C min
-1
Cool
from -18.1°C to -22.2°C at 0.4°C min
-1
Cool
from -22.2°C to -25.3°C at 0.3°C min
-1
Cool
from -25.3°C to -27.8°C at 0.2°C min
-1
Cool
from -27.8°C to -31°C at 0.1°C min
-1
Cool
from -31°C to -34°C at 3°C min
-1
Cool
from -34°C to -40°C at 6°C min
-1
Cool
from -40°C to -100°C at 10°C min
-1
Hold
at -100°C
Transfer to storage
Figure 52: Pre-programmed oocyte
– non-linear freezing cycle
-110
-100
-90
-80
-70
-60
-50
-40
-30
-20
-10
0
10
20
30
0
10
20
30
40
50
60
70
80
90 100 110 120 130 140 150 160 170 180
Time (mins)
Temperature (
degC)
Summary of Contents for CRF-1 H00
Page 1: ...Controlled Rate Freezer CRF 1 Series Operating Manual...
Page 59: ......
