CRF-1 Operating Manual
Page 37 of 60
33801-V4 DMN S90
7.3.1.2. CRF-1 H01
– 0.25ml IMV Straws
The CRF-1 H01 is designed for the controlled rate freezing of 0.25ml capacity straws, for
example the CBS Paillette Cristal. Load up to 18 straws as normal and seal with a straw plug,
cristaseal etc. Thoroughly dry the outside of the straws after preparation to prevent them from
freezing to the sample plate.
Position the straws into the sample plate and fully press them into the grooves (Figure 37).
Place the lid over the sample plate checking that it is located correctly. Straws should be
positioned such that the samples are in the centre of the freezing plate; the straw plug should
overhang the freezing plate if required.
Figure 37: 0.25ml IMV straws positioned on sample plate
7.3.1.3. CRF-1 H02
– 1.8ml Cryovials, 0.5ml max fill
The CRF-1 H02 is designed for the controlled rate freezing of 12.0mm diameter cryovials
including Nunc 363401 and Nunc 340711. The maximum fill for each cryovial is 0.5ml. Load
up to 55 cryovials on the sample plate
.
Cryovials should always be loaded starting at the
centre of the freezing plate. Thoroughly dry the outside of the cryovials after preparation to
prevent them from freezing to the sample plate.
Position the cryovials (Figure 38). Place the lid over the sample plate checking that it is
located correctly.
Figure 38: Cryovials positioned on sample plate
Summary of Contents for CRF-1 H00
Page 1: ...Controlled Rate Freezer CRF 1 Series Operating Manual...
Page 59: ......
