CRF-1 Operating Manual
Page 41 of 60
33801-V4 DMN S90
Remove the lid of the freezer completely.
Turn off the buzzer by pressing the ‘Buzzer Off’
button on the keypad. Touch the nucleating tool (if using the cryopen please refer to the
operating instructions for safe use) to the first sample for approximately one second (Figure
43, Figure 44 & Figure 45). Repeat with the remaining samples and replace the lid of the
freezer. Note that other methods of ice nucleation may also be employed dependent on
experimental procedure.
Inspect the samples approximately one minute after nucleation to verify that there is some
ice-growth. If ice has not formed in any of the samples repeat nucleation.
Figure 43: Nucleation of CBS straws (CRF-1 H00)
Figure 44: Nucleation of IMV straws (CRF-1 H01)
Figure 45: Nucleation of cryovials (CRF-1 H02)
Summary of Contents for CRF-1 H00
Page 1: ...Controlled Rate Freezer CRF 1 Series Operating Manual...
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