Hach BioTector B3500C User Manual Download

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Oxygen Purity Test:

Power up the system for at least 10 minutes before the oxygen purity test is

carried out. Using the Simulate menu (see Section

8.1.2 Simulate

) set the MFC (see figure 2 and

table 2 in Section

4.1.1 Analysis Enclosure

) flow to 10 l/h and flow oxygen gas through the CO

analyzer for 5 minutes. At the end of this period, the CO

analyzer zero reading (ppm CO2)

should be within ±0.5% of full scale of the CO

analyzer range. For instance, if the CO

analyzer

range is 10000ppm, then the CO

analyzer zero reading should be typically within ±50ppm.

(

If the CO

analyzer zero reading is outside the specifications, confirm that there is no CO

in the oxygen gas

by connecting the CO

filter “used with the sodium hydroxide reagent container” between the Cooler and

analyzer inlet port and set the MFC to 10 l/h. As the size of the CO

filter is small, keep the 10 l/h gas

flow running for at least for 5 minutes and record the CO

zero readings at the end of the 5 minute period. If

the CO

zero readings do not drop significantly with the CO

filter in place, this will indicate that there is no

contamination in the oxygen supply.

)

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3. PUMP TESTS:

Caution! Below procedures involves handling strong acid and base reagents. Necessary safety precautions,
such as wearing eye protection and gloves, should be taken throughout these tests.

Go to Zero Calibration menu and select RUN REAGENTS PURGE function to prime the pumps.
The factory Reagents Purge settings to prime reagents typically cover ~3 meters distance
between the reagent containers and the BioTector. If it is necessary to increase the reagent purge
times, see section

8.3.4.4Reagents Purge

for details.

(

If the reagent lines do not fill during the Reagents Purge cycle, stop the BioTector to prime the pumps

manually. Remove the dip tubes from the reagent containers, and seal the containers. Place the dip tubes
into a small container containing DIW. If DIW is not available use tap water. Raise the container above the
height of the BioTector. Go to Zero Calibration menu and select RUN REAGENTS PURGE function to prime
the pumps. Once the pumps have been primed, reinstall the dip tubes into the reagent containers and repeat
the Reagents Purge cycle.)

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Confirm the Acid Pump is pumping correctly using a 10ml graduated cylinder. Run the Acid Pump
in Simulate menu. Acid Pump rate for FMM20 Pump at 400 pulses should be between 4.20 ml
and 3.80 ml.

(Depending on the quantity of the liquid injected into the reactor and due to an internal system

interlock, the system may request the activation of Reactor Purge cycle to purge any excess liquid from the
reactor. If necessary

run “REACTOR PURGE” function in the same menu.)

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Confirm the Base Pump is pumping correctly. Base Pump rate for FMM20 Pump at 400 pulses
should be between 4.20 ml and 3.80 ml.

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Important Note: For the correct operation of the system, the measured Acid and Base Pump rates must be
identical or similar. The maximum allowable difference in the measured volumes for acid and base injections
above should not be more than 0.2ml.

Confirm the WMM60 Sample Pump is pumping correctly. The pump rate at 16 pulses should be
between 5.5ml and 7.5ml in ~8 seconds.

(

Any variation between these pumped volumes is corrected

when the zero and span calibration is carried out.

)

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