Harvard Bioscience Multi Channel Systems SE Smart Ephys MEA, Manual

Page 1: ...1 MEA Signal Generator Manual ...

Page 2: ...all the publisher and the author be liable for any loss of profit or any other commercial damage caused or alleged to have been caused directly or indirectly by this document 2019 Multi Channel Systems MCS GmbH All rights reserved Printed 30 10 2019 Multi Channel Systems MCS GmbH Aspenhaustraße 21 72770 Reutlingen Germany Phone 49 71 21 909 25 0 Fax 49 71 21 909 25 11 sales multichannelsystems com...

Page 3: ...basic understanding of technical and software terms If you are using the device for the first time please read the important safety advice before installing the hardware and software where you will find important information about the installation and first steps The device and the software are part of an ongoing developmental process Please understand that the provided documentation is not always...

Page 4: ...ravention Malfunctions which could impair safety should be rectified immediately High Voltage Electrical cords must be properly laid and installed The length and quality of the cords must be in accordance with local provisions Only qualified technicians may work on the electrical system It is essential that the accident prevention regulations and those of the employers liability associations are o...

Page 5: ... to transport storage installation commissioning operation or maintenance of the device Unauthorized structural alterations to the device Unauthorized modifications to the system settings Inadequate monitoring of device components subject to wear Improperly executed and unauthorized repairs Unauthorized opening of the device or its components Catastrophic events due to the effect of foreign bodies...

Page 6: ...ectrode MEAs 60MEA SG for use with the USB MEA256 System with 256 electrode MEAs 256MEA SG for use with the MEA2100 120 System with 120 electrode MEAs 120MEA SG and for use with wireless systems ME W SG The function of all types is similar Please read the respective datasheets in the Appendix The picture above shows the 60MEA SG Important When using a 60MEA SG within the MEA2100 HS60 or the MEA210...

Page 7: ...ctrode arrays in the middle there is a battery for power supply a blue double DIP switch a plug socket for grounding cable a control button and a LED To start a MEA SG press the control button To stop it press the button for longer than two seconds A flashing LED indicates activity The position of the DIP switch selects different categories of signals see table below Important To change DIP switch...

Page 8: ...n times Signal Source Signal Type OFF OFF 1 The MEA SG is ON Artificial Sine Wave 0 005 Hz Note sine waves 1 Hz might not be visible because of the hardware filter bandwidth 2 Sine Wave 0 01 Hz 3 Sine Wave 0 03 Hz 4 Sine Wave 1 25 Hz 5 Sine Wave 12 5 Hz ON OFF 1 The MEA SG is ON Hippocampal Slice EPSP 2 Hippocampal Slice Population Spike 3 Hippocampal Neurons Spikes OFF ON 1 The MEA SG is ON Heart...

Page 9: ...t carefully 4 Ground the MEA SG with a CB GND cable 4 1 Handling a MEA SG After starting the software in this case MC_Rack and adding a data display you see your selected signal in this case 12 5 Hz sinus waves Please adjust the axes via drop down menus or slider in the header of the display X axis 1000 ms y axis 50 µV MC_Rack data display On this MC_Rack data display channel 66 gets saturated Che...

Page 10: ...the operating mode of the amplifier and you have a tool for troubleshooting 4 3 Training A MEA SG is optimized for training users because you are able to use the software and work with the complete MEA System AMEA SG is also a good basis for demonstration To learn about the software please read the detailed software manuals You can try out different features of the software and their influences on...

Page 11: ... feel comfortable The Multi Channel Suite software package is flexible and intuitive to use and supports all MEA2100 Systems the USB ME data acquisitions and Wireless Systems It consists of three tools Multi Channel Experimenter for data acquisition and online analysis Multi Channel Analyzer for offline analysis Multi Channel DataManager for data export to third party programs Multi Channel Experi...

Page 12: ...data stream will flow from the filter to the recorder The grey input port of the recorder accepts all types of input data streams and you can connect more than one data stream Clicking the button of any instrument with the right mouse provides three options Delete Instrument for deleting the tool Edit Instrument Name for changing the name and Show Info for information about the instrument It is no...

Page 13: ...ams for each instrument separately Generally you will arrange the virtual instruments in your rack in a hierarchical order The selected data streams flow from your MC_Card or from the Replayer recorded data into the virtual instrument highest in the hierarchy Similar to a production line in a plant this instrument picks up only those channels from the data streams that you have assigned to it It p...

Page 14: ...cally when a file has reached the maximum size option Auto Stop As has been said before the fate of each single channel is independent from other channels You can pick specific channels you wish to save from all generated data streams For example you can decide to save only one channel of raw data but the peak to peak amplitude results of all or of a specific selection of channels Replaying Data I...

Page 15: ...pen File Information Click the File Info tab You see the date and time when the file has been recorded and all important information about the conditions which were used Click the Buffer Info tab On the left pane you see the single data stream or several streams which were recorded the number of channels the sampling frequency the gain factor and the data format Saving a Rack Save the rack if you ...

Page 16: ...ounding and shielding the system 7 1 Grounding and Shielding Noise level After mounting a MEA SG connecting it to ground but before switching it on you first see the noise level 50 Hz hum The level should be comparable on each channel not too big and without additional interference If the overall noise is too strong shielding and grounding of the setup is not sufficient Check the grounding cable a...

Page 17: ...the reference electrode If the same electrodes in the MEA layout are affected the contact of the amplifiers electrode pins are bad may be the gold contacts of 60MEA SG too In most cases this is a problem of contamination To repair clean the contact pads of 60MEA SG and electrode pins of the amplifier with a soft tissue or a cotton swab and 100 pure alcohol The channels should now display flawless ...

Page 18: ...120MEA SG or ME W SG 1 Lithium Battery CR 1620 durability maximal 2 years 1 With MEA SG only Grounding Cable CB GND 1 MEA Signal Generator Manual Batteries do not belong in normal household waste and thus must always disposed of within the framework of existing legislation ...

Page 19: ...s on the MCS web site Mailing list If you have subscribed to the mailing list you will be automatically informed about new software releases upcoming events and other news on the product line You can subscribe to the list on the contact form of the MCS web site www multichannelsystems com ...