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Material # 20004364
Document # 15065681 v01
Introduction
Reagent preparation instructions depend on the reagent kit that you are using. Instructions
are organized by flow cell type and include HiSeq X, HiSeq 3000/4000, HiSeq high output,
and HiSeq rapid. For instructions on preparing reagents for a GAIIx flow cell, see the guide
for your cluster kit:
}
TruSeq PE Cluster Kit v2 Reagent Preparation Guide (part # 15019437)
}
TruSeq SR Cluster Kit v2 Reagent Preparation Guide (part # 15019436)
After preparing clustering reagents, you are ready to load them onto the cBot when
prompted by the software.
Best Practices
}
Wear a fresh pair of gloves when preparing clustering reagents.
}
Do not remove the protective clear plastic lid on the reagent plate until you are ready to
load reagents onto the cBot. Never puncture the foil seals.
}
Hold reagent plates that contain 8-tube strips by the plate base to avoid dislodging
reagent tubes. Make sure that the tubes are securely seated in the reagent plate before
and after vortexing or inverting. Loose tubes can damage the cBot manifold.
}
For cluster generation on a HiSeq X or HiSeq 3000/4000 flow cell,
always
prepare
freshly diluted NaOH for denaturing libraries. This step is essential to the denaturation
process. To prevent small pipetting errors, prepare at least 1 ml of freshly diluted 0.1 N
NaOH.
Summary of Contents for cBot
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