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Material # 20004364
Document # 15065681 v01
Introduction
All clustering steps are performed on the cBot except for preparing libraries for sequencing
and preparing the cBot reagent plate. Clustering steps for a rapid flow cell consist only of
template hybridization and first extension. The remaining steps are performed on the
sequencing instrument.
Setting up the cBot for cluster generation without sample tracking includes selecting a
protocol and then loading consumables. All consumables are scanned using the external
barcode scanner or entered manually.
Library Prep
Before setting up the cBot for cluster generation, prepare libraries for sequencing. The
process differs depending on library type and flow cell type.
}
Most libraries on TruSeq flow cells and HiSeq flow cells require a denaturation and
dilution step. For more information, see
HiSeq and GAIIx Systems Denature and Dilute
Libraries Guide (document # 15050107)
.
}
The denaturation protocol differs for HiSeq X and HiSeq 3000/4000 patterned flow cells.
Denature libraries for use with these flow cell types
only
as described in the reagent
preparation instructions for your flow cell type. For more information, see
Summary of Contents for cBot
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