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cBot 2 System Guide
41
Unload Run Components
1
When the run is complete, select
Unload
to proceed.
Figure 17
Run Complete, Unload Components
2
Raise the instrument lid.
3
Release the outlet clamp securing the outlet end of the manifold.
4
Disconnect the outlet end of the manifold from the outlet port in the wash reservoir.
5
Remove the sipper comb from the metal guide pins using the plastic tabs on either side
of the sipper comb.
6
Release the flow cell clamp.
7
Remove the manifold. Make sure that the flow cell remains on the thermal stage.
8
Lift the flow cell from the thermal stage.
9
Store the flow cell as appropriate:
}
TruSeq v2, TruSeq v3, and HiSeq v4 flow cells
—Store in storage buffer in the flow
cell tube at 2°C to 8°C. The flow cell is stable after primer hybridization up to
10 days when properly stored in the flow cell tube.
}
HiSeq and TruSeq Rapid flow cells
—Perform the sequencing run on the same day
as library loading.
}
HiSeq X and HiSeq 3000/4000 flow cell
s—Store in storage buffer up to 48 hours at
2°C to 8°C.
10
Pull the reagent plate lever toward you to release the reagent plate. Remove the reagent
plate from the reagent stage.
WARNING
This set of reagents contains formamide, an aliphatic amide that is a probable
reproductive toxin. Personal injury can occur through inhalation, ingestion, skin
contact, and eye contact. Wear protective equipment, including eye protection,
gloves, and laboratory coat. Handle used reagents as chemical waste and discard in
accordance with the governmental safety standards for your region.
For
environmental, health, and safety information, see the SDS for this kit at
.
11
Remove the 8-tube strip containing libraries.
Summary of Contents for cBot
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