C
lu
s
te
ri
n
g
W
it
h
o
u
t
S
a
m
p
le
T
ra
c
k
in
g
50
Material # 20004364
Document # 15065681 v01
Load Consumables
The software guides you through the steps to load the cBot reagent plate, flow cell, cBot
manifold, and the 8-tube strip containing prepared libraries. Depending on the clustering
protocol selected, the software prompts you to load an 8-tube strip of additional primers.
Load the Reagent Plate
1
Remove the clear plastic lid from the cBot reagent plate.
2
Select
Scan Reagent ID
to activate the external barcode scanner.
3
Raise the instrument lid by lifting from the cutout on the front of the lid.
4
[For TruSeq v3 and TruSeq v2 reagents]
Remove the red foil seal:
a
Hold each end of the tube strip in row 10 and peel the red foil from the 8-tube strip.
Discard the foil appropriately.
b
Select the onscreen checkbox to indicate that the seal is removed.
5
Pull the reagent plate lever toward you and place the reagent plate onto the reagent
stage:
}
All reagent plates except HiSeq X and HiSeq 3000/4000
—Position with row 1
directly behind the tube strip holders. The beveled corner of the plate is positioned in
the front-right corner.
}
HiSeq X and HiSeq 3000/4000 reagent plates
—Position with the barcode label
facing toward the back of the instrument. The beveled corners of the plate are
positioned directly behind the tube strip holders.
Figure 21
Position the Reagent Plate
6
Release the lever to secure the reagent plate.
7
Select the onscreen checkbox to indicate that the reagent plate is loaded, and then select
Next
.
Load the Flow Cell
1
Lift the flow cell clamp.
2
Wash the adapter plate on the thermal stage with a small amount of deionized water,
and wipe dry with a lint-free cleaning tissue.
3
Remove the flow cell from storage:
Summary of Contents for cBot
Page 4: ...iv Material 20004364 Document 15065681 v01 ...
Page 16: ...10 Material 20004364 Document 15065681 v01 ...
Page 22: ...16 Material 20004364 Document 15065681 v01 ...
Page 66: ...60 Material 20004364 Document 15065681 v01 ...
Page 76: ...70 Material 20004364 Document 15065681 v01 ...
Page 88: ...Index 82 Material 20004364 Document 15065681 v01 ...
