©Copyright 2019, Intra-Lock® International, Inc.
LPRF-Protocol Rev. 7 Page 5 of 9
IMPORTANT POINTS TO REMEMBER
- ALWAYS CENTRIFUGE TUBES IN PAIRS: Always place the tubes in pairs and place them in
opposite positions to balance the centrifuge rotor. The tubes must always be balanced in the
rotor before pressing the START button or this may cause serious damage to the centrifuge,
improper coagulation and/or separation. If the tubes are not properly balanced, one will have
too much vibration during centrifugation and a poor clot for L-PRF® will result.
- ODD NUMBER OF BLOOD SAMPLES: If you have an odd number of blood samples to centrifuge,
then place a tube of the same size as the blood samples, filled with water (9 or 10ml depending
on the blood collection tube being used), opposite to the un-paired tube in the rotor. This will
allow for proper balancing of the centrifuge.
- START CENTRIFUGATION: Begin centrifugation immediately after collecting the blood samples.
Delays affect the blood separation procedure and result in a poor clot for L-PRF®
.
- AFTER CENTRIFUGATION: Remove the rubber stopper from each tube and place the tubes onto
the sterile rack.
L-PRF
®
Preparation
- After centrifugation, three segments are visible:
1. Upper Segment = platelet poor plasma (PPP).
2. Middle Segment = fibrin clot: L-PRF®.
3. Lower Segment= red blood cell clot.
- L-PRF® fibrin matrix must be prepared relatively quickly: 0-15
minutes after centrifugation or the clot will shrink in volume by
releasing the trapped serum.
- Remove L-PRF® clot from the tube with forceps.
- Cut the L-PRF® clot just below the union with the red blood cell clot. This will leave a very small
portion of red blood cells and large platelets attached to L-PRF® clot.
Summary of Contents for IntraSpin Series
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