iota Sciences isoCell, User Manual

Page 1: ...V1 0r1 isoCell user manual For software versions 1 0 0 1 0 8 ...

Page 2: ...he chambers has been reduced from 800nl to 600nl This will provide greater chamber stability Chambers are still plated with 200nl but Fill will now only add 400nl to the chambers rather than 600nl Other programs have been modified to work with the lower volume chambers but are otherwise unchanged ...

Page 3: ...utions Only use the isoCell once you have read and understood this manual Ensure this manual is accessible for all users and that each potential user reads and understands it Take all suitable precautions recommended for the safe handling of all cells and other reagents used For the case of FC40STAR users should familiarise themselves with the FC40 material safety data sheet which is available on ...

Page 4: ...he isoCell safety may be compromised Ensure that no spilled liquid has been in contact with or entered the isoCell If water or other liquid enters the isoCell stop using it immediately and unplug the power supply Continued use may cause a fire Contact iotaSciences for actions or precautions If any foreign object enters the isoCell safety may be compromised Contact iotaSciences for actions or preca...

Page 5: ... is by default undeployed and therefore may not be visible The isoCell is supplied with the following accessories consumables and spares Documents isoCell quickstart user guide isoCell training guide Single cell suspension preparation guidelines Mains power cable with appropriate mains plug for country of use Transformer 24V Mean Well GST90A24 P1M power supply ...

Page 6: ... 18x 60mm dishes either tissue culture treated or suspension with attached locating rings 36x eight tube PCR strips or 36x flat bottom 8 well strips 50x 1 5ml microcentrifuge tubes 1x pipetting aid 2x dispensing assembly Training kit comprising 6x tissue culture treated 60mm dishes with attached locating rings 12x eight tube PCR strips 25x 1 5ml microcentrifuge tubes 1x pipetting aid 1x 10 page si...

Page 7: ...on the top of the isoCell The isoCell bed placement of reagents and materials The isoCell bed Figure 2 is where the workflow of isolating culturing and harvesting cells is executed Figure 2 The isoCell bed showing correct layout of 60mm dish microcentrifuge tubes and 2x 8 tube PCR strips optionally replaced with 2x flat bottom 8 well strips The workflow is executed on a 60mm tissue culture dish su...

Page 8: ...crocentrifuge tubes are required Users should ensure that waste tubes are empty For instructions on the safe disposal of FC40STAR refer to Disposal of FC40STAR Cells are harvested by the isoCell from the 60mm dish into the two 8 tube PCR strips or flat bottom 8 well strips located at the front of the bed Figure 2 The isoCell will provide a visual prompt when the PCR or flat bottom strips are requi...

Page 9: ...t the isoCell into a fresh state It is recommended that when switching users the new user restarts the isoCell in this way Powering off To power off the isoCell first press the on screen button located on the left side of the touchscreen Then select the shutdown option which will initiate a shutdown routine At the end of the shutdown routine the isoCell will indicate that power can be safely switc...

Page 10: ...lture dish with each chamber measuring 1 9mm by 1 9mm 2 The plate program is used to dispense 200nl cell suspension fluid to each of the GRID s 256 chambers 3 The input data program is used to store in the isoCell s memory the GRID coordinates of chambers containing single cells GRID To form a GRID the user must first prepare the tissue culture dish with a coating of cell culture media supplement ...

Page 11: ...when plating sequentially since the dispensing system is washed and sterilised between dishes Input data Once cells have been plated into chambers the chambers that contain single cells need to be identified and the corresponding GRID coordinates need to be determined This is facilitated through use of the reference stage supplied on which each chamber s GRID coordinate is etched Remove the plated...

Page 12: ... 200nl to 800nl This must be the first program that is run on the dish following the input data program Feed The feed program is used to remove 800nl waste from each monoclonal chamber and replace the removed fluid with 800nl fresh media The user may run this program as often as they deem suitable The feed program should not be run on a dish containing suspension cells this would result in the cel...

Page 13: ...m into each tube well Do not put caps on the PCR strips Chamber states As a dish progresses through the workflow the isoCell tracks the state of the chambers The current state of chambers in a dish is displayed at the beginning of the input data fill feed detach and harvest programs and can be viewed at any time via the data program The chamber states are Empty Chamber is empty This is the default...

Page 14: ... does not record the number if any of times the feed program has been run on a dish The feed program will operate on chambers in a single cell filled state and will return them to a single cell filled state Advanced programs Data The data program more data enables the user to Add or remove usernames View current chamber states of dishes Erase data of individual dishes under a chosen username Remov...

Page 15: ...rate program must be run to calibrate the new dispensing needle FC40STAR level The FC40STAR level program more FC40STAR level is used to update the tracked level of FC40STAR in the FC40STAR bottle located at the rear of the isoCell To update the level move the slider to the correct height and press the button to save See Refilling FC40STAR for more information Reset jetting The reset jetting progr...

Page 16: ...ive to the centre of the isoCell bed It can be used if either of the isoCell s needles has been replaced to ensure continued performance of the isoCell Needle calibration happens automatically during the startup and restart routines and when replacing the dispensing assembly using the replace dispensing program Move The move program more move is used to Manually move the isoCell s needle holder us...

Page 17: ...Figure 3a Needle holder control screen ...

Page 18: ...ences Maintenance Cleaning Users are recommended to clean the exterior of the isoCell regularly ideally before and after each use iotaSciences recommend the use of a paper towel dampened with 70 ethanol Do not attempt to manually clean the needles which are delicate and easily damaged The isoCell programs if followed correctly will ensure that needles are kept clean ...

Page 19: ...fresh FC40STAR into the bottle It is not necessary to remove any residual FC40STAR Having topped up the bottle replace the two port cap Then use the on screen interface to update the tracked FC40STAR level Alternatively the tracked FC40STAR level can be updated at any time using the FC40STAR level program Ensure that the FC40STAR intake tube is submerged at the bottom of the FC40STAR bottle Replac...

Page 20: ...ropean Union EU implemented the EU WEEE Directive 2002 96 EC and later the WEEE Recast Directive 2012 19 EU requiring Producers of electronic and electrical equipment EEE to manage and finance the collection reuse recycling and to appropriately treat WEEE that the Producer places on the EU market after August 13 2005 The goal of this directive is to minimize the volume of electrical and electronic...

Page 21: ...stomer portal FC40STAR must not be discarded into municipal drains sewers Disposal of reagents and consumables Used reagents and consumables other than FC40STAR should be discarded in a suitable biohazard container Refer to your local waste management organisation and to the relevant laboratory safety instructions for proper disposal practices Technical support For technical questions support issu...

Page 22: ...pe of the above warranty In case of any discrepancy between the above warranty and said full terms and conditions said full terms and conditions will take precedence To make a warranty claim please contact Iota Sciences Ltd at techsupport iotasciences com before returning your instrument to us Limited liability To the maximum extent permitted by law unless otherwise stated in our terms and conditi...

Page 23: ...ss or purpose ii in compliance with environmental health and safety regulations and iii will not infringe any third party s intellectual property rights Medical use restrictions The isoCell is intended for research use by professionals only The isoCell is not intended to be used for diagnostic purposes or for testing or treatment in humans Registered office Iota Sciences Ltd Begbroke Science Park ...

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