The AFC measures the volume of each fraction by weight as it fills with liquid eluting from the column.
When the designated threshold weight is reached, the fluid flow is automatically stopped to prevent
dripping of fluid during repositioning of the carousel.
The type of qEV column used in each run will depend on the user’s preferences and research
applications. For more information on which column to use, see
Section 6: qEV Specifications
and for
more information on choosing a collection schedule, see
Section 7: Choosing Run Parameters.
Intended Use:
The AFC is used to automate fraction collection of Izon’s qEV size-exclusion columns.
The instrument is intended to be used in research laboratories by professional personnel for research
purposes only. Neither the AFC nor the qEV column are intended for diagnostic purposes and should not be
used to make treatment decisions.
For verification of the entire system, it is recommended that Good Laboratory Practices (GLP) be followed to
ensure reliable analyses.
Figure 1: Typical elution profile for the qEVoriginal.
Larger extracellular vesicles (EVs) and exosomes elute first, while smaller
vesicles and protein complexes elute later; 500 µL of bovine plasma on a qEVoriginal; concentration of EVs measured with a
qNano and TRPS; concentration of proteins measured with spectrophotometry.
*
Volumes are labelled as the highest volume in that sample i.e label “0.5” refers to the volume from 0.0-0.5 mL after the buffer
volume, label “1.5” refers to the volume from 1.0-1.5 mL after the buffer volume and so on.
Volume (mL)
0.25
0.15
0.30
0.20
0.10
0.05
0.00
1.4
1.0
1.6
1.2
0.8
0.6
0.4
0.0
0.2
C
onc
en
tr
ation (EV
s/
m
L x 10
10
)
Plasma pr
otein (Abs 280 nm
)
Buffer Volume
PCV
0.5
1.5
6.5
5.5
4.5
3.5
2.5
Automatic Fraction Collector User Manual
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