Jasco V-530, Instruction Manual

Page 1: ...P N 0302 0304C January 2003 V 530 550 560 570 Spectrophotometer Instruction Manual V 500 forW indows...

Page 2: ...symbol refer to the instrument manuals to protect the instrument against damage WARNING A WARNING indicates an potentially hazardous situation which if not avoided could result in death or serious inj...

Page 3: ...ning Labels on the V 530 Back Panel WARNING FOR CONTINUED PROTECTION AGAINST RISK OF FIRE REPLACE ONLY WITH FUSE OF THE SPECIFIED TYPE AND CURRENT RATINGS Only use fuses of the specified rating to pro...

Page 4: ...0 Back Panel 2 Warning for carrying The weight in kilograms of each instrument is as follows V 530 V 550 V 560 V 570 16 0 30 5 32 0 33 0 When moving the instrument hold the handgrips at the bottom of...

Page 5: ...l process measurement and control equipment Part 4 Electrical fast transient burst requirements Severity level 3 IEC1010 1 1990 Amd 1 1992 Amd 2 1995 Safety requirements for electrical equipment for m...

Page 6: ...this manual in a safe place If this instruction manual becomes lost order a replacement from your local JASCO distributor The instruction manual is divided into following volumes 1 Hardware manual De...

Page 7: ...cy The instrument may not function properly when near a strong magnetic field or high frequency source 8 Avoid vibration from vacuum pumps electric motors processing equipment and machine tools 9 Avoi...

Page 8: ...ftware Copying software for purposes other than backup Transfer or licensing of the right to use software to a third party Disclosure of confidential information regarding software Modification of sof...

Page 9: ...TS BEYOND JASCO S CONTROL INCLUDING NATURAL DISASTERS This warranty does not cover the consumable parts listed below 1 Deuterium lamp tungsten lamp xenon lamp and other light sources 2 Mirrors in the...

Page 10: ...nd Spectrum Measurement 10 3 1 Quantitative Analysis Introduction 10 3 1 1 Quantitative analysis program overview 10 3 1 1 1 Quantitative analysis program 10 3 1 1 2 Quantitative analysis operation 11...

Page 11: ...y Text 44 4 4 4 Invalid 44 4 4 5 Data Input 44 4 4 6 Title 44 4 5 View menu 44 4 5 1 Font 45 4 5 2 Format 46 4 5 3 Cell Width 46 4 5 4 Scale 46 4 5 5 Pattern 47 4 5 6 Grid 47 4 5 7 Style 48 4 5 8 Mark...

Page 12: ...Help menu 69 8 Abs T Meter 70 9 Environment 72 9 1 Hardware Setting 72 9 2 Diagnosis menu 73 9 3 Accessories Setting 74 9 4 Wavelength Correction 75 10 Appendix 76 10 1 Spectra Manager Installation 76...

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Page 14: ...PC W i ndow s and V 500W as w el las exi ti ng V 500W and W i ndow s and shutti ng dow n the spectrophotom eter Speci fi c program operati ons are descri bed i n subsequent secti ons Thi s secti on a...

Page 15: ...ssion Drag Point to an item click and hold down the mouse button Move the mouse with the button held down and release the button when the pointer is where you want it 1 3 O verview of Spectra M anager...

Page 16: ...program 1 Spectra A nal ysi s program Thi s program saves pri nts and processes di fference spectrum peak pi cki ng sm oothi ng deri vati ve verti calaxi s conversi on and so on spectrum data orti m e...

Page 17: ...w eri s turned O N the pow erl am p on the spectrophotom eteri s l i t The l i ghtsource needs aboutfi ve m i nutes to becom e stabl e Then m easurem entm ay begi n 2 1 2 PC and W i ndow s startup Tur...

Page 18: ...enu Ifan opti onalappl i cati on program has been i ncl uded i t w i l lal so appeari n the Spectra M anager w i ndow See Secti on 2 3 Spectra M anager M enu fora ful l expl anati on 3 D oubl e cl i...

Page 19: ...how to shutdow n the spectrophotom eterand PC 2 2 1 Exi ti ng m easurem entorspectra anal ysi s program Ends the M easurem ent or A nal ysi s program and exi ts i t 1 Exi ti ng the Spectra A nal ysi...

Page 20: ...Exi ti ng W i ndow s Exi tW i ndow s accordi ng to the W i ndow s U ser s G ui de 2 2 2 PC and spectrophotom etershutdow n 1 Turn O FF the pow erto the PC and di spl ay D o notforgetto turn offthe di...

Page 21: ...rom the Instrum ent l i st M enu A ppl i cati on m enu A nal ysi s Starts the A nal ysi s program M easurem ent Starts the M easurem ent program Exi t Exi ts the Spectra M anager w i ndow and returns...

Page 22: ...m ati on of the control dri ver of the spectrophotom eter H el p m enu C ontents D i spl ays the hel p C ontents w i ndow Search Topi c D i spl ays the keyw ord w i ndow A bout D i spl ays the versi o...

Page 23: ...program overvi ew 3 1 1 1 Quantitative analysis program The quanti tati ve anal ysi s program has the fol l ow i ng features 1 Three m ethods ofanal ysi s outl i ned 1 1 w avel ength quanti tati ve an...

Page 24: ...i tati ve anal ysi s m ethod and m easurem entparam eters Setcal i brati on curve param eters and i nputstandard sam pl e concentrati on See Secti on 3 1 3 M easure standard sam pl e bl ank M easure s...

Page 25: ...to the spectrophotom eter W hen transferi s fi ni shed the program starts and the fol l ow i ng w i ndow appears Fi gure 3 3 Q uanti tati ve anal ysi s w i ndow 3 1 3 C al i brati on curve creati on 1...

Page 26: ...row to the ri ghtofthe box to di spl ay the ful ll i st ofopti ons To sel ect Fast response cl i ck Fast C hange otherparam eters i fnecessary 2 C l i ck the 1 w avel ength opti on button ofthe M etho...

Page 27: ...data di spl ay fi el d and the cursorm oves to the nextl i ne autom ati cal l y N ote Ifthe absorbance ofthe standard sam pl e i s know n m easuri ng the standard sam pl e i s notnecessary Inputthe ab...

Page 28: ...d the C al i brate C urve Param eters di al og box i s cl osed the standard bl ank val uei ssubtractedfrom theabsorbanceval ueofthestandardsam pl e Thestandardbl ankandstandard sam pl e can be m easur...

Page 29: ...al i brati on curve i s created by cl i cki ng M ethod N ew onl y the C al i brate C urve and M ethod Inform ati on w i ndow s open Fi gure 3 9 D ata Sheet w i ndow 3 1 4 C al i brati on curve m odi f...

Page 30: ...rem entparam eters 1 C l i ck M ethod Save A s The fol l ow i ng di al og box appears Fi gure 3 10 Save Param eters di al og box 2 I nputa fi l enam e to the Param eterN am e textbox The fi l enam e m...

Page 31: ...i brati on curve di spl ayed i n the w i ndow The resul ts appearon the D ata Sheet 4 R epeatsteps 2 and 3 as m any ti m es as the num berofsam pl es N ote Thesam pl ebl anki ssubtractedfrom theabsorb...

Page 32: ...ecessary afterthe N ote C l i ck C om m ents to open the C om m ents di al og box Sam pl enam e operator and organi zati on can be i nputi fnecessary 3 C l i ck Save to save the data sheetto di sk 3 1...

Page 33: ...3 1 9 Exi ti ng quanti tati ve anal ysi s C l i ck Fi l e Exi t to return to the Spectra M anager w i ndow afterm easurem enti s fi ni shed N ote Ifunsaved D ata Sheet and or C al i brate C urve data...

Page 34: ...starts the Spectra A nal ysi s program and the spectra are di spl ayed i n the acti ve vi ew Spectra can be saved orpri nted i n the Spectra A nal ysi s program Spectrum m easurem ent program start S...

Page 35: ...og box The param eters can be changed accordi ng to the exam pl es bel ow Photom etri c M ode T M easuri ng w avel ength range 600 to 400 1 C hangi ng photom etri c m ode The Photom etri c M ode i s...

Page 36: ...N ote W hen an opti onalaccessory i s i nstal l ed i n the sam pl e cham ber the opti calpath changes Thus the basel i ne m ustbe re m easured 1 C l i ck M easurem ent Basel i ne The fol l ow i ng di...

Page 37: ...er i s em pty C l i ck Start to begi n m easurem ent The m easured basel i ne i s saved i n m em ory A tthe sam e ti m e the Basel i ne C orrecti on checkbox i s sel ected To confi rm thatthe Basel i...

Page 38: ...ure 3 20 Sam pl e cham ber 2 C l i ck M easurem ent Start or cl i ck the Start button The sam pl e i s m easured and the m easurem entprogressi on appears W hen m easurem enti s f i ni shed the Spectr...

Page 39: ...e Fi l e N am e textbox U p to 8 characters can be i nput The extensi on i s not requi red afterthe 3 C l i ck Save to save spectra to di sk 3 2 7 Pri nti ng resul ts Spectra can be pri nted usi ng a...

Page 40: ...spectrum Repeatprocedure accordi ngl y 2 Exi ti ng the Spectrum M easurem ent program C l i ck M easurem ent Exi t The Spectrum M easurem ent w i ndow cl oses and the Spectra M anager w i ndow reappea...

Page 41: ...Calibration Curve and Method Information windows must be opened in order to display this window Method Information Displays information including measurement parameters calibration curve data and comm...

Page 42: ...eys Ti tl e edi ts the D ata Sheet col um n ti tl e V i ew m enu Font Sets the fontforthe D ata Sheet orcal i brati on curve Form at Sets the num berofdeci m alpl aces to appearon the D ata Sheet C el...

Page 43: ...M easurem ent Param eters di al og box A new quanti tati ve anal ysi s m ethod fi l e can be added See Secti on 4 2 1 N ew V i ew D i spl ays detai l s ofthe currentl y sel ected quanti tati ve anal...

Page 44: ...1 Fi gure 4 3 I nform ati on di al og box Fi gure 4 4 D ata Sheet w i ndow A n unknow n sam pl e can be m easured from the D ata Sheet w i ndow usi ng the di spl ayed quanti tati ve anal ysi s m ethod...

Page 45: ...i rectory appear i n the drop dow n l i stbox Fi l e nam e l i stbox Sel ecta fi l enam e Fi l e nam e Textbox fori nputti ng a fi l enam e The fi l enam e can al so be sel ected from the fi l enam e...

Page 46: ...cl i ck the fi l enam e Fi l e nam e I nputnam e of D ata Sheet fi l e to be saved I fthe extensi on i s om i tted the setFi l e Type extensi on i s affi xed autom ati cal l y I fan exi sti ng fi l en...

Page 47: ...x i tem s such as Param eters and G raph can be sel ected for pri nti ng from thi s group A check m ark nextto the i tem i ndi cates thati tw i l l be pri nted Font O pens the Font di al og box 4 1 6...

Page 48: ...s i n dpi w hi ch i s the num berofdots peri nch 2 4 cm The hi gher the num ber the hi gherthe resol uti on Setup Sets the targetpri nter and pri nti ng condi ti ons for thatpri nter The sam e procedu...

Page 49: ...ange vari es accordi ng to the m odel V 530 Fi xed at2 nm V 550 560 0 1 0 2 0 5 1 2 5 10 nm V 570 0 1 0 2 0 5 1 2 5 10 nm 0 4 0 8 2 4 8 20 40 nm neari nfrared regi on N ote W hen usi ng theV 570 form...

Page 50: ...ysi s m ethod accordi ng to num berofw avel engths Peak Peak w avel ength Base 1 Base 1 w avel ength Base 2 Base 2 w avel ength The i nputrange vari es accordi ng to the m odel V 530 190 0 to 1100 0...

Page 51: ...Q uanti tati ve M easurem ent di al og box Enabl e Bl ank Sel ect the Enabl e Bl ank checkbox w hen the standard bl ank val ue i s i nput A check i s autom ati cal l y appended w hen the standard bl...

Page 52: ...pl e are m easured from thi s di al og box Fi gure 4 15 Q uanti tati ve M easurem ent di al og box Standard Sel ectthe Standard opti on button to m easure a standard sam pl e Bl ank Sel ectthe Bl ank...

Page 53: ...gth to zero O K Exi ts the C al i brate C urve Param eters di al og box and opens the C al i brate C urve and M et hod I nform ati on w i ndow s Fi gure 4 17 C al i brate C urve and M ethod I nform at...

Page 54: ...tati ve anal ysi s m ethod C om m ents Sam pl e nam e operator copyri ght and com m entcan be i nputor edi ted The fol l ow i ng di al og box appears Fi gure 4 19 C om m ents di al og box I nput Sam p...

Page 55: ...ank i s updated the new val ue i s appl i ed onl y to al l subsequentl y m easured unknow n sam pl es N ote To see the currentsam pl e bl ankval ue open the Bl ankC orrecti on di al og box see Secti o...

Page 56: ...ycl e can al lbe changed See Secti on 4 2 1 N ew to see the Q uanti tati ve M easurem ent Param eters di al og box 4 3 3 Bl ank C orrecti on C l i ck M easurem ent Bl ank C orrecti on to set w hether...

Page 57: ...ntrati on i s cal cul ated accordi ng to the currentquanti tati ve anal ysi s m ethod and i s w ri tten to the concentrati on fi el d The D ata I nput di al og box can al so be opened by doubl e cl i...

Page 58: ...ppears Fi gure 4 26 FontSetti ng di al og box I tem Li sts the i tem s forw hi ch the fontcan be set A xi s l abel A l phabeti c characters for I ntensi ty or C oncentrati on Scal e l abel N um eri c...

Page 59: ...dual l y foreach col um n Thi s functi on i s acti ve onl y w hen the D ata Sheet w i ndow i s acti ve Fi gure 4 28 I nternati onalN um berForm at di al og box 4 5 3 C el lW i dth C l i ck V i ew C el...

Page 60: ...C ol or Show s avai l abl e col ors Sel ecta col orfrom thi s pal ette The l i ne seti n the El em ent l i stw i l lbe di spl ayed i n the sel ected col or Li ne Styl e Show s avai l abl e l i ne styl...

Page 61: ...33 Styl e Setti ngs di al og box A xi s Li sts the axes for w hi ch the styl e can be set Sel ect C oncentrati on or A bsorbance Scal e Label The scal e i nt ervaland num berofdeci m alpl aces can be...

Page 62: ...he Fi l lI nsi de checkbox to fi l lthe i nsi de ofthe m arker A s D efaul t Sel ect the A s D efaul t checkbox to use the set m arker patterns i n subsequentdi spl ays i n the C al i brate C urve w i...

Page 63: ...th di al og box w hi ch i s used to m ove the w avel ength to an arbi trary w avel ength D oubl e cl i cki ng the photom etri c val ue di spl ay di spl ays the Param eters di al og box w hi ch i s use...

Page 64: ...Param eters di al og box A tthe sam e ti m e the spectra anal ysi s program starts N ote1 Afteracti vati ngspectraanal ysi sandstarti ngi ni ti alm easurem ent thenextvi ew show i ngtheresul tscovers...

Page 65: ...m ul ti pl i er Inputrange i s 0 to 1000 V R esponse R esponse by si m pl e m ovi ng average Sel ectabl e range vari es accordi ng to the m odel V 530 Q ui ck M ovi ng average duri ng about0 03 sec Fa...

Page 66: ...s a pai rw i th End I nputrange vari es accordi ng to the m odel V 530 190 0 to 1100 0 nm V 550 560 190 0 to 900 0 nm V 570 190 0 to 2500 0 nm End Shorterw avel ength end ofthe m easurem entw avel eng...

Page 67: ...l i m i ts ofthe verti calaxi s range di spl ayed on the screen I f A uto i s sel ected the ful l scal e axi s i s setto about1 2 ti m es the m axi m um w i dth of the di spl ayed spectrum based on th...

Page 68: ...gure 5 5 Param eters O pen di al og box Param eters Li st Li sts avai l abl e saved param eters O K Loads sel ected param eter s from m em ory C ancel R eturns to the Param eters di al og box w i thou...

Page 69: ...f i t i s not saved nothi ng w i l lbe di spl ayed here Ful lW avel ength Puta check m ark w hen m easuri ng orusi ng the ful lbasel i ne R em ove the check m ark w hen m easuri ng orusi ng a parti al...

Page 70: ...ng thi s opens the fol l ow i ng di al og box Fi gure 5 9 Param eters Save di al og box Enteri ng a fi l enam e and cl i cki ng O K saves the basel i ne onto the di sk M easure M easures the basel i n...

Page 71: ...to the spectrophotom eter W hen m easurem ent fi ni shes a check m ark i s puti n the Basel i ne C orrecti on check box and the Param eters Save di al og box Fi g 5 7 opens Si m ul taneousl y the bas...

Page 72: ...ti ng V 570 Fi gure 5 13 Exchange W avel ength di al og box W avel ength forLam p Exchange Sets the deuteri um l am p and hal ogen l am p exchange w avel ength Entera w avel ength i n the textbox I np...

Page 73: ...ftto ri ght D oubl e cl i cki ng the w avel ength di spl ay opens the G oto W avel ength di al og box i ti s used form ovi ng the w avel ength to an arbi trary w avel ength D oubl e cl i cki ng the ph...

Page 74: ...m easurem ent thenextvi ew show i ngtheresul tscovers the Spectrum Vi ew and hi des i t To save the hi dden Spectrum Vi ew save orpri nti t To di spl aythe hi dden Spectrum Vi ew agai n change the app...

Page 75: ...ui ck M ovi ng average duri ng about0 03 sec Fast M ovi ng average duri ng about0 25 sec M edi um M ovi ng average duri ng about1 sec V 550 560 570 Q ui ck M ovi ng average duri ng about0 03 sec Fast...

Page 76: ...i m es the m axi m um w i dth of the di spl ayed spectrum based on the m easurem ent resul t O K Ends param eter setti ng and cl oses the di al og box C l i ck thi s button to transferthe setparam ete...

Page 77: ...64 6 2 Help m enu A bout D i spl ays i nform ati on such as program versi on...

Page 78: ...eftto ri ght D oubl e cl i cki ng the w avel ength di spl ay di spl ays the G oto W avel ength di al og box used for m ovi ng the w avel ength to an arbi trary w avel ength D oubl e cl i cki ng the p...

Page 79: ...C orrect M easuri ng the sam pl e i n thi s condi ti on subtracts the bl ank val ue I f bl ank correcti on i s notneeded rem ove the check m ark from Bl ank C orrect C orrect Starti ng the second and...

Page 80: ...ded to the l eft si de Inputthe vol tage to be appl i ed to the photom ul ti pl i er The i nputrange i s 0 to 1000 V R esponse R esponse by si m pl e m ovi ng average Sel ectabl e range vari es accord...

Page 81: ...ta w avel ength i n the textbox 2 C l i ck A dd C hange R ew ri tes w avel ength i n the di spl ay fi el d H ow to change w avel ength 1 Sel ecta w avel ength from the di spl ay fi el d 2 I nputnew w...

Page 82: ...es the spectrophotom eterw avel ength to a setw avel ength see Secti on 5 1 4 M ove W avel ength 7 1 5 A uto Zero Sets absorbance ortransm i ttance atthe currentw avel ength to zero or100 T fortransm...

Page 83: ...he m eterpoi nter i s fi xed i n posi ti on thi s button changes to Start C l i ck thi s button a second ti m e to return to ori gi nal status Thi s functi on i s useful for obtai ni ng data w hen the...

Page 84: ...g about4 sec Band w i dth Spectralbandw i dth Sel ectabl e range vari es accordi ng to the m odel V 530 Fi xed at2 nm V 550 560 0 1 0 2 0 5 1 2 5 10 nm V 570 0 1 0 2 0 5 1 2 5 10 nm 0 4 0 8 2 4 8 20 4...

Page 85: ...photom eterstatus A ccessori es Setti ng Sets the condi ti ons of the opti onal i ntel l i gent accessori es that have com m uni cati ng functi ons W avel engt h C orrect i on C orrect s t he w avel e...

Page 86: ...g changeoverw avel ength G rati ng Exchange forthe ul travi ol et vi si bl e regi on and forthe near i nfrared regi on I nputthe w avel ength i n the textbox I nputrange 750 to 900 nm D efaul tval ue...

Page 87: ...w i th peri stal ti c pum p si pper D el ay W ai ti ng ti m e before m easurem ent from sam pl e sucti on or end of pum pi ng to startofm easurem ent D rai n Sam pl e di scharge ti m e D i recti on Se...

Page 88: ...xternal A l l ow s tem perature m easurem entusi ng externalsensordi pped i n the cel l Sam pl e tem perature can be m easured di rectl y 9 4 W avelength Correction C orrects the w avel ength usi ng t...

Page 89: ...e W i ndow s 95 98 N T4 0 2000 U ser s G ui de Referto the W i ndow s 95 98 N T4 0 2000 Instructi on M anualfordetai l s on how m uch di sk space i s requi red 10 1 2 I nstal l i ng Spectra M anagerfr...

Page 90: ...77 Fi gure 10 2 C ontrolPanel 2 D oubl e cl i ck A dd R em ove Program s i n the C ontrolPanel w i ndow The w i ndow bel ow appears Fi gure 10 3 A dd R em ove Program s Properti es...

Page 91: ...stal l I button The w i ndow bel ow appears Fi gure 10 4 I nstal lProgram From Fl oppy D i sk orC D R O M 4 C l i ck the N ext button i n the w i ndow show n i n Fi g 10 4 The w i ndow show n i n Fi g...

Page 92: ...Inputting operator name or company name 1 I nputthe nam e ofthe operator or com pany i n the textbox show n i n Fi g 10 7 The nam e thatyou i nputi s regi stered i n the system Thi s procedure can be...

Page 93: ...ry nam e shoul d be used c j ascow 32 2 C l i ck the N ext button The screen show n i n Fi g 10 9 appears Fi gure 10 9 D ata D i rectory i nputscreen 10 1 2 4 Setting the data directory 1 I nputthe dr...

Page 94: ...Fi g 10 13 appears Fi gure 10 13 M odelnam e and seri alnum beri nputscreen 6 I nputthe m odelnam e and seri alnum beri fnecessary C l i ck the N ext button 10 1 2 5 Copying files to hard disk The Se...

Page 95: ...ck Setup 3 The m essage I fthi s i s the correcti nstal l ati on program w i l lappear H ere cl i ck Brow se Fi gure 10 15 4 Sel ectC D R O M dri ve Spectra M anager D i sk1 Setup cl i ck O K and the...

Page 96: ...i cati ons C l i ck start on the taskbar and proceed to program then Jasco then Spectra M anagersetup and cl i ck to run program Fi gure 10 18 7 Sel ect appl i cati on from the m enu box and cl i ck...

Page 97: ...teri s control l ed through anotherport The procedure i s as fol l ow s Fi gure 10 20 Spectra M anager w i ndow 1 C l i ck I nstrum ents PortSetti ng on the Spectra M anager m enu The di al og box sho...

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Page 99: ...JASCO Corporation 2967 5 Ishikawa machi Hachioji TOKYO Japan Printed in Japan...