c.
Hint: For easier operation, in HV mode, use the SEI detector to set up the
instrument and focus, etc. Switch to the BEIW mode mostly to look at or record
slow scan images.
d.
If you are working at high magnification, select a smaller spot size for a sharper
image. If the image is too noisy select a larger spot size. Remember there is a
trade off between noise (fast scanning) and attainable resolution.
e.
Tilt and Z must be adjusted only if you are sure it is safe to do so (do not run
into detectors!!). Use the IR chamber-scope always for these adjustments!!
f.
Scan Rotation is available (SRT icon). This is very useful for orientating the
image the way you want without rotating the stage (be aware the perception of
the image can change due to the apparent direction of oblique illumination
changing (relative position of the detector). It is also very useful for working
with cross-section samples (changing the orientation of the tilt axis).
g.
Watch out for Tools
Æ
Neutralizer being checked. When neutralizer mode is
selected the voltage on the collector grid is changed. This mode reduces
secondary electron collection of the lowest energy electrons and may help with
some kinds of charging artifacts.
h.
Many commonly used functions are available on the knob panel (magnification,
brightness, contrast, stigmator x and y, image fine shift x and y, scanning mode,
and auto functions {focus, stigma, contrast brightness}).
i.
Scan4 (80 seconds) will automatically freeze at end of frame to save image.
While scanning the image observed is just a preview, the actual image quality is
much better (just watch the image as the scan finishes, then press the Zoom
button)
j.
Scan3 (10 or 20 seconds) will freeze at end of frame if freeze is clicked.
k.
Photo (160 seconds) can be used to decrease noise and get the best possible
quality.
l.
Saved images are 1280 x 960 pixels.
For Scan3 the images are 640 x 480(10 sec) or 1280 x 960 (20 sec pixels).
m.
In the Save Image dialog box there is a check box,
Merge Text. When this is
checked the saved image will have the data area text embedded in the image.
n.
Displayed images are 640 x 480. Click Zoom on frozen image to see full
resolution image (1024 x 768) or (1280 x 960 with pan).
o.
Measuring functions are available under the Image menu for a frozen image.
The measurements can be burned into the image with the Write button prior to
saving the image.
p.
SIS AnalySIS software (on SEM computer) can be also used to do image
measurements.
q.
ImageJ (Java version of NIH Image) is free software which also can be used
for image analysis on your own computer.
